Molecular stool screening for colorectal cancer

Mak, Terrence; Lalloo, Fiona; Evans, D. Gareth; Hill, James A.

doi:10.1002/bjs.4576

Cited by 31 publications

(30 citation statements)

References 91 publications

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“…Stool DNA testing is a non-invasive method for detecting exfoliated markers (13)(14)(15)(16)(17)(18). High-throughput molecular profiling also shows some potential in stool molecular screening for colorectal cancer (19)(20)(21) (22). Cooperation between microbial and mammalian metabolisms is an essential element in the study of the human metabolome (23).…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling of fecal water extracts from human colorectal cancer

et al. 2008

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Colorectal cancer is the second leading cause of cancer death in developed countries. There is a need for better preventive strategies to improve the outcome of this disease. The increasing availability of high-throughput methodologies opens up new possibilities for screening new markers. The application of NMR metabolic profiling to fecal water extracts has interesting potential as a diagnostic tool for detecting colorectal cancer. We obtained NMR metabolic profiles of fecal water extracts from patients with colorectal cancer and healthy individuals, to characterize possible differences between them and to identify potential diagnostic markers. Our results show that metabolic profiling of fecal water extracts is a cheap, reproducible and effective method for detecting colorectal cancer markers and therefore complements other stool-screening methods. A low concentration of short-chain fatty acids, such as acetate and butyrate, previously associated with the development of colorectal cancer, appears to be the most effective marker. Concentrations of proline and cysteine, which are major components of most colonic epithelium mucus glycoproteins, also display significant changes in samples from colorectal cancer. Differentiation between fecal water extracts from controls and patients with colorectal cancer by NMR spectroscopy combined with chemometric techniques opens up new possibilities for developing new, efficient, high-throughput screening protocols.

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Section: Introductionmentioning

confidence: 99%

Metabolite profiling of fecal water extracts from human colorectal cancer

et al. 2008

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“…El análisis genético a partir de ADN extraído de heces es complicado y engorroso, pues generalmente el ADN obtenido se encuentra contaminado con restos propios del material fecal, de la dieta del animal o suele estar en bajas cantidades o degradado (Deuter et al, 1995; Kohn et al, 1995;Mak et al, 2004;Panasci et al, 2011). El uso de un método adecuado de extracción es fundamental, no solamente para eliminar impurezas, pero sobre todo, para evitar la inhibición en la amplificación de productos en pruebas de PCR.…”

Section: Resultados Y Discusiónunclassified

OPTIMIZACIÓN DE UNA TÉCNICA PARA LA EXTRACCIÓN DE UN ADN DE HECES DE VICUÑA (Vicugna vicugna mensalis).

Maturrano

Aguilar²,

Krüger³

et al. 2012

Rev. investig. vet. Perú

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RESUMENActualmente es posible determinar el estatus de especies silvestres mediante el análisis de ADN extraído de muestras no invasivas como las heces. Aunque dichos análisis suelen ser dificultosos debido principalmente a la composición y conservación de las heces, se ha demostrado en muchas especies que con el desarrollo de métodos adecuados de extracción es posible maximizar la fiabilidad y eficacia de este procedimiento. Por tanto, el objetivo del presente estudio fue optimizar un método de extracción de ADN de heces de vicuña Vicugna vicugna mensalis, especie clasificada como casi amenazada en el Perú, con la finalidad de obtener ADN de suficiente calidad para análisis moleculares. Se recolectaron 51 muestras de heces durante los meses de agosto y septiembre de 2008 en dos comunidades vicuñeras ubicadas a más de 4600 msnm en los departamentos de Junín y Moquegua. Luego de observar la defecación, las muestras fueron recolectadas en forma inmediata, conservadas en etanol y transportadas al laboratorio para la extracción del ADN. El kit comercial QIAamp® DNA Stool Mini Kit (QIAGEN) fue modificado para incluir un vigoroso y extenso proceso de agitación y los resultados fueron comparados con dos protocolos comúnmente utilizados: fenol/cloroformo/alcohol isoamílico y el mismo kit QIAGEN. La cantidad del ADN extraído fue observado en electroforesis horizontal en geles de agarosa, y la calidad evaluada mediante la amplificación y visualización de los tamaños esperados de los microsatélites YWLL46 y LCA19. Aunque se logró extraer ADN con los tres métodos, solamente el método modificado permitió obtener ADN de suficiente calidad para ser utilizado en pruebas moleculares. Palabras clave: extracción de ADN, heces, vicuña ABSTRACTAt present, the use of DNA extracted from non-invasive samples, especially feces, is common practice in the discipline of conservation genetics. Even though DNA analysis is often difficult mainly due to the composition and preservation of feces has been

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“…Knowledge of the reasons for non-attendance of males would be useful. With appropriate counselling these nonattendees may be prepared to undergo colonoscopy or willing to try other screening tests such as stool DNA testing if available in the future [17,18]. Interestingly where a mutation has been identified the proportion of males coming forward for testing is much closer to 50%.…”

Section: Discussionmentioning

confidence: 96%

Familial colorectal cancer referral to regional genetics department—a single centre experience

et al. 2006

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Evaluation of familial colorectal cancer referral can assist in the planning of future cancer surveillance. The aim of the study is to assess colorectal cancer referral pattern to our regional genetics service. Hospital computer records and/or department referral books were used to identify cases referred to the regional genetic service during a 10-year period (1992-2001 inclusive). All files were reviewed along with associated demographic data, risk assessments, referral details, results from mutation testing and screening recommendations. In terms of result, a total of 1100 family files were reviewed (Familial Adenomatous Polyposis families were in a separate register). The number of referrals showed a 10-fold increase over the 10 years. 171 (15.6%) of families met the Amsterdam criteria II were classified as high-risk, 589 (53.5%) families were classified as moderate-risk and 337 (31.0%) as average or low risk. 22.9% families were referred with inaccurate cancer history. Sixty-one families have been identified with mismatch repair mutations. 56.8% of referred individuals were recommended to have regular colonoscopy ranging from 18 monthly to 5 yearly depending on their risks. In conclusion, there has been a 10-fold increase in individuals with suspected hereditary bowel cancer referred to the North West Regional Genetics Service in the last ten years. Genetic assessment may reduce the number of low-risk individuals and those who were found not to be mutation carriers from having unnecessary colonoscopic screening. Thus genetic risk assessment should precede the initiation of regular endoscopic screening.

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Molecular stool screening for colorectal cancer

Abstract: Recent studies on molecular stool-based testing have shown higher sensitivity and specificity than earlier studies, but larger clinical trials are required. Laboratory methods are still undergoing research, with the aim of improving sensitivity to allow large-scale testing.

Cited by 31 publications

References 91 publications

Metabolite profiling of fecal water extracts from human colorectal cancer

Metabolite profiling of fecal water extracts from human colorectal cancer

OPTIMIZACIÓN DE UNA TÉCNICA PARA LA EXTRACCIÓN DE UN ADN DE HECES DE VICUÑA (Vicugna vicugna mensalis).

Familial colorectal cancer referral to regional genetics department—a single centre experience

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