Molecular spectroscopic study of DNA binding with neutral red and application to assay of nucleic acids

Wang, Yating; Zhao, Feiyang; Li, Ke An; Shen, Tong

doi:10.1016/s0003-2670(99)00365-7

Cited by 87 publications

(27 citation statements)

References 20 publications

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“…This technique has been developed [65][66][67][68][69][70] as a sensitive technique for the detection of bioorganic complexes. The resonance elastic light scattering from molecules and nanoparticles in solution results from the absorption of photons followed by an immediate coherent re-emission of light in all directions without any energy loss [70].…”

Section: Introductionmentioning

confidence: 99%

Resonance elastic light scattering (RELS) spectroscopy of fast non-Langmuirian ligand-exchange in glutathione-induced gold nanoparticle assembly

Stobiecka

Coopersmith

Hepel

2010

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Resonance elastic light scattering (RELS) spectroscopy of fast non-Langmuirian ligand-exchange in glutathione-induced gold nanoparticle assembly

Stobiecka

Coopersmith

Hepel

2010

Journal of Colloid and Interface Science

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“…(13,14) As a new spectral analysis technique, the resonance light scattering (RLS) measurement using a common spectrofl uorometer, which can compensate for the drawbacks of spectrophotometric and spectrofl uorometric measurements, has gained wide application in the detection of biomolecules, such as nucleic acids and protein, (15)(16)(17)(18)(19) saccharides and (20) metallic ions. (21) In addition, RLS technique has been widely and successfully applied to determine DNA in real and artifi cial samples with various resonance light scattering probes, such as organic dyes, (22,23) cation porphyins, (24,25) metal complexes, (26,27) antibiotic drugs, (28) nanoparticles (29,30) and surfactants. (31,32) A series of polycations, poly[acrylamide-(2-methylacryloxyethyl) dimethyl buthylammonium bromide] [P (AM-DMB)], were polymerized used acrylamide and (2-methylacryloxyethyl) dimethyl buthylammonium bromide.…”

Section: Introductionmentioning

confidence: 99%

Spectral study of interaction between poly [acrylamide‐(2‐methyl acryloxyethyl) dimethyl buthylammonium bromide] and nucleic acids

2011

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A polycation, poly[acrylamide-(2-methylacryloxyethyl) dimethyl buthylammonium bromide] [P (AM-DMB)], was synthesized. The interaction between P (AM-DMB) and DNA was studied by spectral methods. Resonance light scattering spectra, FTIR-spectra and UV-spectra were used to study the mechanisms of the interaction. The results indicate that the conformation of the DNA alters during the interaction because the microenvironment of DNA changes. The binding constant K between P (AM-DMB) and DNA is 8.0 × 10(3) L mol(-1) .

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“…Resonance scattering technique, using common spectrofluorimeter, was simple, sensitive and inexpensive, and has been widely applied in analysis of nucleic acids, proteins, and small molecular drug [28][29][30][31][32][33][34][35][36], with satisfactory results.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Resonance Scattering Detection of DNA Hybridization Using Aptamer-Modified Gold Nanopaticle as Catalyst

et al. 2010

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Gold nanoparticle particles in size of 10 nm were used to label the thiol-modified single-stranded DNA aptamer (SH-ssDNA) to obtain an aptamer-modified gold nanoparticle probe (AussDNA) for target DNA (tDNA). In pH 7.4 NaH 2 PO 4 -Na 2 HPO 4 buffer solution, the hybridization reaction between AussDNA and tDNA took place to form larger aptamer-modified gold nanoparticle cluster complex. The excess aptamer-modified gold nanoparticle probe in the supernatant solutions was obtained by centrifuging and can be used as nanocatalyst for the 0.276 mmol/L CuSO 4 -65.4 mmol/L potassium-sodium tartrate-0.37 mmol/L glucose system at 70°C. The cubic Cu 2 O particles generated by the nanocatalytic reducing exhibit a strong resonance scattering (RS) peak at 620 nm. In the selected conditions, the RS intensity at 620 nm decreased with addition of tDNA, and the decreased intensity ΔI 620 nm is proportional to tDNA concentration (C tDNA ) from 0.12 to 72 pM, with regress equation of ΔI 620 nm =1.29C tDNA + 4.05, correlation coefficient of 0.9917, and detection limit of 0.084 pM tDNA.

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Molecular spectroscopic study of DNA binding with neutral red and application to assay of nucleic acids

Cited by 87 publications

References 20 publications

Resonance elastic light scattering (RELS) spectroscopy of fast non-Langmuirian ligand-exchange in glutathione-induced gold nanoparticle assembly

Resonance elastic light scattering (RELS) spectroscopy of fast non-Langmuirian ligand-exchange in glutathione-induced gold nanoparticle assembly

Spectral study of interaction between poly [acrylamide‐(2‐methyl acryloxyethyl) dimethyl buthylammonium bromide] and nucleic acids

Highly Sensitive Resonance Scattering Detection of DNA Hybridization Using Aptamer-Modified Gold Nanopaticle as Catalyst

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