Molecular Spectroscopic Markers of DNA Damage

Sofińska, Kamila; Wilkosz, Natalia; Szymoński, Marek; Lipiec, Ewelina

doi:10.3390/molecules25030561

Cited by 34 publications

(24 citation statements)

References 129 publications

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“…A similar observation has been described for pulmonary hypertension rat model [51]. Sofińska et al reported that increasing intensity of phosphate vibration indicates DNA fragmentation [52]. High kidney DNA fragmentation has .…”

Section: Discussionsupporting

confidence: 73%

Infrared spectral markers for the nephroprotective effects ofFicus deltoideain streptozotocin-induced diabetic rats

Samsulrizal

Ahmad

Azmi

et al. 2020

Preprint

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Fourier Transform Infrared (FTIR) is an established analytical technique to elucidate new discriminatory biomarkers. Our previous study showed that Ficus deltoidea (Ficus: Moraceae) is capable of increasing insulin secretion and improving tissue regeneration by reducing oxidative stress in diabetic rats. However, the assessment of treatment response is limited by the paucity of biomarkers. We aimed to evaluate the potential use of FTIR for assessing the nephroprotective effects of Ficus deltoidea (Ficus: Moraceae) in diabetic rats. A rat model of diabetes was induced using a single intraperitoneal injection of streptozotocin (STZ) (60 mg/kg body weight). Methanolic extract of F. deltoidea was administered orally at a dose of 1000 mg/kg body weight for eight weeks. Fasting blood glucose, serum insulin and kidney function parameters were examined. The kidneys were subsequently subjected to FTIR and histological analyses. Enzyme-linked immunosorbent assays (ELISA) assessed the levels of oxidative stress, antioxidant, and apoptosis-related proteins in the kidney tissue. The results show, for the first time, that there is a good agreement between changes in kidney and FTIR peaks. The IR peaks (1545 cm−1 and 1511 cm-1) corresponding to amide II were restored by treatment with F. deltoidea. Multivariate analysis demonstrated that the diabetic rats treated with F. deltoidea had similar clustering pattern that of the normal animals. Biochemical and histological examination further confirmed the nephroprotective effect of F. deltoidea. Thus, demonstrating how FTIR spectroscopy could be used for the diagnosis of diabetes kidney disease.

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Section: Discussionsupporting

confidence: 73%

Infrared spectral markers for the nephroprotective effects ofFicus deltoideain streptozotocin-induced diabetic rats

Samsulrizal

Ahmad

Azmi

et al. 2020

Preprint

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“…Further, UV light also has an antimicrobial effect on the RNA of viruses, including coronaviruses ( 5 ), which further increases our need to understand the chemical basis for the biological effects of UV light. DNA damage, such as deamination, oxidative damage, strand breaks, and dimer formation, can hinder normal functioning of a cell, prevent DNA replication, and cause cell death ( 6 ). UV-induced DNA damage results mainly in damage to pyrimidine bases, thus inducing the formation of cyclobutane pyrimidine dimers (CPDs), pyrimidine(6-4)pyrimidone photoproducts, and Dewar isomers ( 7 ).…”

mentioning

confidence: 99%

Thymine dissociation and dimer formation: A Raman and synchronous fluorescence spectroscopic study

Nagpal

Dhankhar

Cesario³

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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In this study, absorption, fluorescence, synchronous fluorescence, and Raman spectra of nonirradiated and ultraviolet (UV)-irradiated thymine solutions were recorded in order to detect thymine dimer formation. The thymine dimer formation, as a function of irradiation dose, was determined by Raman spectroscopy. In addition, the formation of a mutagenic (6-4) photoproduct was identified by its synchronous fluorescence spectrum. Our spectroscopic data suggest that the rate of conversion of thymine to thymine dimer decreases after 20 min of UV irradiation, owing to the formation of an equilibrium between the thymine dimers and monomers. However, the formation of the (6-4) photoproduct continued to increase with UV irradiation. In addition, the Raman spectra of nonirradiated and irradiated calf thymus DNA were recorded, and the formation of thymine dimers was detected. The spectroscopic data presented make it possible to determine the mechanism of thymine dimer formation, which is known to be responsible for the inhibition of DNA replication that causes bacteria inactivation.

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“…Multimodal imaging could be used to monitor oxidative stress, a common consequence of exposure to some toxicants, including nanoparticles, in cells and organoid models through DNA damage and changes in lipid peroxidation. 38 For this, Raman can monitor DNA conformational changes following several bands between 600 and 1700 cm –1 , 39 while MSI can monitor lipid peroxidation by identifying lipid species and their degree of saturation. 40 Therefore, cell response can be characterized in situ as a living system (with VSI) and as a frozen metabolic state (with MSI).…”

Section: Multimodal Imaging and Its Transition To The Clinicmentioning

confidence: 99%

Perspective on Multimodal Imaging Techniques Coupling Mass Spectrometry and Vibrational Spectroscopy: Picturing the Best of Both Worlds

et al. 2021

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Studies on complex biological phenomena often combine two or more imaging techniques to collect high-quality comprehensive data directly in situ , preserving the biological context. Mass spectrometry imaging (MSI) and vibrational spectroscopy imaging (VSI) complement each other in terms of spatial resolution and molecular information. In the past decade, several combinations of such multimodal strategies arose in research fields as diverse as microbiology, cancer, and forensics, overcoming many challenges toward the unification of these techniques. Here we focus on presenting the advantages and challenges of multimodal imaging from the point of view of studying biological samples as well as giving a perspective on the upcoming trends regarding this topic. The latest efforts in the field are discussed, highlighting the purpose of the technique for clinical applications.

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Molecular Spectroscopic Markers of DNA Damage

Cited by 34 publications

References 129 publications

Infrared spectral markers for the nephroprotective effects ofFicus deltoideain streptozotocin-induced diabetic rats

Infrared spectral markers for the nephroprotective effects ofFicus deltoideain streptozotocin-induced diabetic rats

Thymine dissociation and dimer formation: A Raman and synchronous fluorescence spectroscopic study

Perspective on Multimodal Imaging Techniques Coupling Mass Spectrometry and Vibrational Spectroscopy: Picturing the Best of Both Worlds

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