Molecular Species of Glycerolipids of Adenosine Triphosphatase and Sarcotubular Membranes of Rabbit Skeletal Muscle

Abstract

The molecular species of choline, ethanolamine, serine, and inositol phosphatides and triglycerides were determined in purified adenosine triphosphatase and in the parent sarcotubular membranes of rabbit skeletal muscle. The total membrane and the adenosine triphosphatase were essentially identical in the composition of lipid classes and closely similar in patterns of molecular species of the corresponding phosphatides and triglycerides. The phosphatidylcholines were made up largely of the 1-palmitoyl 2-oleoyl and 1-palmitoyl 2-linoleoyl species. The ethanolamine phospholipids contained mainly the plasmalogens 1-palmitaldehyde 2-arachidonoyl and 1-stearaldehyde 2-arachidonoyl along with smaller amounts of the 1-palmitaldehyde 2-oleoyl and 1-palmitaldehyde 2-linoleoyl species. The phosphatidylinositols, which were analyzed along with about 10% phosphatidylserine, were characterized by large proportions of 1-stearoyl 2-arachidonoyl and smaller but significant proportions of the 1-stearoyl 2-oleoyl, 1-stearoyl 2-linoleoyl, and 1-stearoyl 2-eicosatrienoyl species. The triglycerides contained significant proportions of tripalmitin, triolein, and the isomeric palmitoyl oleoyl linoleins.The composition and molecular association of the fatty acids in these phosphatides showed significant differences from that of other tissues of the rabbit and other animals, as far as they have been analyzed, except for phosphatidylinositol, the structure of which was similar to that found elsewhere. The triglyceride composition was unusual in the high content of simple triglycerides.

Effects of diet on the function of sarcoplasmic reticulum

“…The fatty acid composition that we find for rabbits fed on a normal diet ( (1 973a,b), particularly for the phosphatidylethanolamine fraction. This presumably can be attributed to differences in the fatty acid content of the diets used by Marai & Kuksis (1973a) and ourselves. Nevertheless, we find an approximately 1: 1 ratio of saturated to unsaturated fatty acyl chains, as reported by Marai & Kuksis (1973a).…”

“…was on silica gel G (1040 ,um particle size) spread as a 1 mm layer on glass plates, activated by heating at 120°C for 1 h. Phospholipids were separated by using chloroform/methanol/water/ethanoic acid (65:45:4:2, by vol.) (Marai & Kuksis, 1973a) Raheja et al (1973). Fatty acids were converted into methyl esters by using sodium methoxide (Glass, 1971) or, for analysis of plasmalogens, converted into a mixture of methyl esters and dimethylacetals by using 10% (v/v) H2SO4 in methanol at 80°C for 4 h (Marai & Kuksis, 1973a).…”

“…(Marai & Kuksis, 1973a) Raheja et al (1973). Fatty acids were converted into methyl esters by using sodium methoxide (Glass, 1971) or, for analysis of plasmalogens, converted into a mixture of methyl esters and dimethylacetals by using 10% (v/v) H2SO4 in methanol at 80°C for 4 h (Marai & Kuksis, 1973a). Fatty acid/aldehyde composition was determined by g.l.c.…”

Changes in the Structure, Composition and Function of Sarcoplasmic-Reticulum Membrane during Development

“…The main fraction of lecithin of embryonic muscle microsomes is a monoenoic species (M. G. Sarzala and M. Pilarska, unpublished), whereas in sarcoplasmic reticulum of adult animals the main fraction is a dienoic species [7,37]. Also in chicken higher amounts of saturated and monoenoic fatty acids were found by Boland et al [I51 in phospholipids from embryonic muscle microsomes than from sarcoplasmic reticulum of adult animals.…”

Molecular Species of Ethanolamine Phosphatides of Dog and Pig Kidney

“…Pure ethanolamine phssphatides were isolated by T.L.C. ' on silica gel H (Merck & Co.) plates (20 dB 228 cm, 250 pm thick layer) in chioroform-methanohater, 65 :25 :4, which carried the neutral lipids to the top and retained the phosphatidylcholiw near the origin, leaving the ethanolamine phssphatides to ascend about 60(:I, of the height of the plate (13,14). The ethanslamine phospholipids were specifically located by spraying the plate with ninhydrin.…”