Molecular Signaling Interactions and Transport at the Osteochondral Interface: A Review

Silva, Mateus Oliveira; Ansari, Niloufar; Stok, Kathryn S.

doi:10.3389/fcell.2020.00750

Cited by 28 publications

(20 citation statements)

References 92 publications

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“…Although OA has long been viewed as a primary disorder of articular cartilage, subchondral bone is attracting increasing attention [39]. The pathology at the cellular level of the chondrocyte and the biochemistry within the knee joint have been discussed in extensive detail elsewhere [12,40,41]. In this section, we present an overview of the key pathological features involved in the osteochondral unit.…”

Section: Pathogenetic Profilesmentioning

confidence: 99%

Role of the Osteochondral Unit in the Pathogenesis of Osteoarthritis: Focus on the Potential Use of Clodronate

Molfetta

Casabella²,

Rosini

et al. 2022

CRR

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: Osteoarthritis (OA) is a chronic disease characterized by inflammation and progressive deterioration of the joint. The etiology of OA includes genetic, phlogistic, dismetabolic and mechanical factors. Historically, cartilage was considered the target of the disease and therapy was aimed at protecting and lubricating the articular cartilage. The osteochondral unit is composed of articular cartilage, calcified cartilage, and subchondral and trabecular bone, which work synergistically to support the functional loading of the joint. Numerous studies today show that OA involves the osteochondral unit, with the participation therefore of the bone in the starting and progression of the disease, which is associated with chondropathy. Cytokines involved in the process leading to cartilage damage are also mediators of subchondral bone edema. Therefore, OA therapy must be based on the use of painkillers and bisphosphonates for both the control of osteometabolic damage and its analgesic activity. Monitoring of the disease of the osteochondral unit must be extensive, since bone marrow edema can be considered as a marker of the evolution of OA. In the present review we discuss some of the pathogenetic mechanisms associated with osteoarthritis, with particular focus on the osteochondral unit and the use of clodronate.

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Section: Pathogenetic Profilesmentioning

confidence: 99%

Role of the Osteochondral Unit in the Pathogenesis of Osteoarthritis: Focus on the Potential Use of Clodronate

Molfetta

Casabella²,

Rosini

et al. 2022

CRR

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“… 2015 ; Oliveira Silva et al. 2020 ). We previously demonstrated that distinct changes occurred with the progression of osteoarthritis (OA) (Fan et al.…”

Section: Introductionmentioning

confidence: 99%

A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology

Fan

Lee

Jones

et al. 2022

Histochem Cell Biol

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The anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protocol elucidates the distinct distribution of elements at the human knee’s osteochondral junction, making it a useful tool for analyzing the co-distribution of various elements in both healthy and diseased states.

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“…This hyaline cartilage is both avascular and aneuronal. It is made of cells called chondrocytes that synthetize their own surrounding extracellular matrix (ECM) [ 1 , 2 ]. The mechanical properties of articular cartilage depend on its ECM and allow joint surfaces to resist wear [ 1 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Hop Extract Anti-Inflammatory Effect on Human Chondrocytes Is Potentiated When Encapsulated in Rapeseed Lecithin Nanoliposomes

Velot

Ducrocq

Girardeau

et al. 2022

IJMS

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Hop (Humulus lupulus L.) is a plant used as an ingredient in beer or employed for its anti-inflammatory properties. The cultivation of hops is currently dedicated to the brewing industry, where mainly female flowers are used, whereas aerial parts, such as leaves, are considered coproducts. Osteoarthritis is the most common musculoskeletal disease associated with low-grade cartilage inflammation. Liposomes have been shown to be promising systems for drug delivery to cartilage cells, called chondrocytes. The aim of our work was to vectorize hop extract valorized from coproducts as a therapeutic agent to alleviate inflammation in human chondrocytes in vitro. Liquid chromatography allowed the identification of oxidized bitter acids in a methanolic extract obtained from the leaves of Cascade hops. The extract was encapsulated in rapeseed lecithin nanoliposomes, and the physicochemical properties of empty or loaded nanoliposomes exhibited no difference. Increasing concentrations of the hop extract alone, empty nanoliposomes, and loaded nanoliposomes were tested on human chondrocytes to assess biocompatibility. The appropriate conditions were applied to chondrocytes stimulated with interleukin-1β to evaluate their effect on inflammation. The results reveal that encapsulation potentiates the hop extract anti-inflammatory effect and that it might be able to improve joint inflammation in osteoarthritis. Furthermore, these results also show that a “zero waste” chain is something that can be achieved in hop cultivation.

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Molecular Signaling Interactions and Transport at the Osteochondral Interface: A Review

Cited by 28 publications

References 92 publications

Role of the Osteochondral Unit in the Pathogenesis of Osteoarthritis: Focus on the Potential Use of Clodronate

Role of the Osteochondral Unit in the Pathogenesis of Osteoarthritis: Focus on the Potential Use of Clodronate

A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology

Hop Extract Anti-Inflammatory Effect on Human Chondrocytes Is Potentiated When Encapsulated in Rapeseed Lecithin Nanoliposomes

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