Molecular sieving of tetraalkylammonium cations on cation exchange systems in DC electric field

Polezhaev, Petr; Belloň, Tomáš; Kurospajeva, Nazerke Chroust; Vobecká, Lucie; Slouka, Zdeněk

doi:10.1016/j.seppur.2020.116691

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…Earlier experimental studies ,− did suggest this strong sensitivity of the microvortices on the surface curvature. In addition, theoretical studies by Chang et al also suggested that a Dukhin type curvature-induced polarization can produce intensified vortices of specific size related to the curvature, in contrast to the randomly sized vortices produced by electroosmotic instability. , The Slouka group visualized the dynamic patterns of microvortices through fluorescently labeled melamine resin microparticles to confirm that the flat or convex geometry of the ion exchange membrane surface would affect the slope of the over-limiting region due to the discrepancy of the patterns of microvortices.…”

Section: Results and Discussionmentioning

confidence: 86%

“…However, the HDA-5 membrane sensor with even more DNA probes immobilized on the surface showed an unexpected lower voltage shift than the HDA-4 membrane sensor. The formation of many small microvortices near the convex membrane surface might also account for the smaller voltage shift than that on the flat membrane, as demonstrated in the studies by Slouka’s group. − The membrane sensors with the flat surface were more sensitive to target DNA attachment and presented a significant voltage shift. The same effect also happened in our experimental results so that the HDA-4 membrane sensor showed a higher voltage shift than the HDA-5 membrane sensor (Figure d,e).…”

Section: Results and Discussionmentioning

confidence: 94%

“…Schematic mechanism of microvortices at different surface curvatures on top of a 500 μm membrane (illustrative drawing sketched according to refs and − ).…”

Section: Results and Discussionmentioning

confidence: 99%

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Novel Homogeneous Anion Exchange Membranes for Reproducible and Sensitive Nucleic Acid Detection via Current–Voltage Characteristic Measurement

Chuang

Diao

Huang

et al. 2020

ACS Appl. Mater. Interfaces

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One-pot synthesis of novel hydrogel-based anion exchange membranes (AEMs), with only a single-phase monomer mixture, was used to eliminate surface heterogeneity and generate reproducible electroconvective microvortices in the over-limiting region of the current−voltage characteristic (CVC) curves. Diallyldimethylammonium chloride (DDA) was used as the main component to provide the cation charge groups, and 2hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethyl acrylate (EGDMA) were used as the auxiliary structure monomers. The uniform membrane structure allowed reproducible and sensitive DNA detection and quantification, as probe−target surface complexes can gate the ion flux and produce large voltage shifts in the over-limiting region. Suppressed membrane curvature due to controlled swelling is a crucial part to avoid the reduction of depletion region for maintaining the influence of target gene hybridization. Fourier-transform infrared (FTIR) spectroscopy verified the synthesized membrane structure, with a residual vinyl group that allows easy carboxylation via additional photografting reaction. Consequently, a significantly higher DNA probe functionalization efficiency is obtained on the homogeneous AEMs, evidenced by the increasing nitrogen element content and bonding via X-ray photoelectron spectroscopy (XPS). The DDA content was optimized to provide a sufficient coulomb force between AEM and nucleic acid backbone to promote the specific binding efficiency but without high dimensional swelling which might change the surface geometry and restrict the voltage shifting for sensing in the over-limiting region, and the optimal DDA/ HEMA ratio was found to be 4/10. The synthesized AEM sensor for recombinant 35S promoter sequence identification exhibited a reproducible calibration standard curve with dynamic range between 30 fM and 1 μM and high selectivity with only 0.01 V shift for 1 μM nontarget oligo.

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Section: Results and Discussionmentioning

confidence: 86%

Section: Results and Discussionmentioning

confidence: 94%

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Novel Homogeneous Anion Exchange Membranes for Reproducible and Sensitive Nucleic Acid Detection via Current–Voltage Characteristic Measurement

Chuang

Diao

Huang

et al. 2020

ACS Appl. Mater. Interfaces

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“…A complex reaction mixture containing soluble enzymes can also alter the distribution of the electric field strength at the ion‐exchange membranes separating the electrode reservoirs. For example, the sorption of charged molecules or changes in the ionic strength (Leinweber & Tallarek, 2004) have a significant effect on the ion‐exchange membrane behavior (Belloň, Polezhaev, Vobecká, & Slouka, 2019; Polezhaev et al, 2020). Ion‐depleted zones forming at one side of the ion‐exchange membranes locally increase electric field strength (Postler et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Despite the limited precision of the Bradford assay, it seems that the sorption of PA to the dialysis membrane does not significantly affect the process of microextraction. (Leinweber & Tallarek, 2004) have a significant effect on the ion-exchange membrane behavior Polezhaev et al, 2020). Ion-depleted zones forming at one side of the ion-exchange membranes locally increase electric field strength (Postler et al, 2008).…”

Section: Continuous Flow Synthesis Coupled With Product Separationmentioning

confidence: 99%

Electric‐field‐enhanced selective separation of products of an enzymatic reaction in a membrane micro‐contactor

Romanov

Slouka

Přibyl

2020

Biotech & Bioengineering

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Processes employed in separations of products of enzyme reactions are often driven by diffusion, and their efficiency can be limited. Here, we exploit the effect of a direct current (DC) electric field that intensifies mass transfer through a semipermeable membrane for fast, continuous, and selective separation of electrically charged molecules. Specifically, we separate low‐molecular‐weight reaction products (phenylacetic acid, 6‐aminopenicillanic acid) from the original reaction mixture containing a free enzyme (penicillin acylase). The developed microfluidic dialysis‐membrane contactor allows a stable counter‐current arrangement of the retentate and permeates liquid streams on which DC electric field is perpendicularly applied. The applied electric field significantly accelerates the transport of electrically charged products through the semipermeable membrane yielding high separation efficiencies at short residence times. The residence time of 5 min is sufficient to reach 100% separation yield in the electric field. The same residence time provides only a 50% yield in the diffusion‐controlled experiments. We experimentally demonstrated that a combined microreactor–microextractor with a recycle of the soluble penicillin acylase can continuously produce both the reaction products at high concentrations. The developed membrane‐contactor is a versatile platform allowing to tune its characteristics, such as selectivity given by the membrane, or the type of the retentate phase, for a specific application.

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Experimental 3D concentration profiles along an electrodialysis channel reveal a strong effect of natural convection

et al. 2023

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Molecular sieving of tetraalkylammonium cations on cation exchange systems in DC electric field

Cited by 10 publications

References 39 publications

Novel Homogeneous Anion Exchange Membranes for Reproducible and Sensitive Nucleic Acid Detection via Current–Voltage Characteristic Measurement

Novel Homogeneous Anion Exchange Membranes for Reproducible and Sensitive Nucleic Acid Detection via Current–Voltage Characteristic Measurement

Electric‐field‐enhanced selective separation of products of an enzymatic reaction in a membrane micro‐contactor

Experimental 3D concentration profiles along an electrodialysis channel reveal a strong effect of natural convection

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