Molecular sieving action of the cell membrane during gradual osmotic hemolysis

Abstract

LBL-5634Rat erythrocytes restrained in either glass filters or a specially designed continuous-flow centrifuge were hemolyzed by exposing the cells to a flowing medium in which the concentration of NaCl, LiCl or dextrose was gradually decreased. This process was termed "controlled gradual osmotic hemolysis." The medium was collected in serial fractions after