Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods

Morinha, Francisco; Cabral, João Alexandre; Bastos, Estela

doi:10.1016/j.theriogenology.2012.04.015

Cited by 105 publications

(88 citation statements)

References 109 publications

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“…Genetically-based sexing is very reliable (Griffiths et al 1998;Morinha et al 2012), but relatively expensive and requires a specially equipped laboratory and time for conducting the genetic analyses. This method requires the capture of birds for sampling blood, feather or saliva, although some advanced techniques allow gene samples to be taken from materials obtained noninvasively, such as molt feathers, feces and the inner layer of egg shells (Jensen et al 2003;Regnaut et al 2006;Balkiz et al 2007;Mudrik et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Gender identification using acoustic analysis in birds without external sexual dimorphism

Volodin

Volodina²,

Klenova

et al. 2015

Avian Res

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Zoo and wildlife management faces a problem with bird sexing, as many bird taxa have indiscernible gender differences in size and coloration. Problematic groups are geese, cranes, rails, raptors, owls, parrots, doves, auks, shearwaters and some passerines. Commonly accepted invasive sexing techniques based on genetics, laparoscopy, morphometric and on cloacal inspection, are all needed in bird capturing and handling. Capturing and subsequent manipulations may be inapplicable for free-ranging birds, whereas distant voice-based sexing is relevant for many species. This review evaluates the potential for noninvasive sexing by separate calls or duet calls, for adult birds of 69 species from 16 orders and for chicks of 11 species from 7 orders. For adult birds of 25 species, a single call per individual was sufficient for 100 % reliable sexing by ear or using spectrographic analysis. For chicks, the potential for voice-based sexing seems to be very limited. For birds calling rarely or unpredictably, we propose a simple way of provoking vocalization using playbacks of species-specific calls that are available from sound libraries. We conclude that sexing by voice may represent a feasible alternative to the classical sexing techniques, both in the wild and in captivity.

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Section: Introductionmentioning

confidence: 99%

Gender identification using acoustic analysis in birds without external sexual dimorphism

Volodin

Volodina²,

Klenova

et al. 2015

Avian Res

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“…While further promising DNA sexing methods are being developed (see Morinha et al, 2012 for a review), this can already be integrated as a conservation tool for this species.…”

Section: Discussionmentioning

confidence: 99%

Using PCR-RFLP for sexing of the endangered Galápagos petrel (Pterodroma phaeopygia)

Patiño¹,

Cruz²,

Martı́nez³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Pterodroma phaeopygia is a critically endangered avian species of the Galápagos Islands. This bird is sexually monomorphic, making it difficult to identify the sex. This information, however, is relevant to studies of behavior, ecology, and management of wild or captive populations. Here, we aimed to implement a molecular approach for determining sex in this petrel. DNA was extracted from the blood and the feathers of 24 adult P. phaeopygia, with samples from a female and a male Gallus gallus for comparison. We amplified the cromo-helicase DNA binding protein 1 (CHD-1) gene by PCR, using primers P2 and P8. Allele CHD-1W is unique to females and CHD-1Z occurs in both sexes. We then digested these PCR products using the restriction enzyme HaeIII. The PCR amplified a 400-bp product for both alleles. The digestion of the G. gallus, amplicons split the CHD-1Z allele into two fragments (of 320 and 80 bp), while CHD-1W 4761 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 4760-4767 (2013) Sexing the Galapagos petrel remained intact. Thus, the male exhibited two bands (digested CHD-1Z) and the female three bands (undigested CHD-1W and digested CHD-1Z). Applying this RFLP method on DNA derived from blood, 9 of the 24 petrels were found to be male, while 15 were females. The same results were obtained using feathers as the source of DNA. To our knowledge, this is the first report of molecular method for sexing this species. The potential of sexing this petrel from feathers is remarkable as it minimizes blood sampling induced stress. This method could be used to reinforce the conservation efforts for this bird, to investigate population sex ratios and to develop new conservation strategies.

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“…A continuous effort was made in PCR-based sex determination techniques to increase the accuracy, speed and high-throughput applicability is evident through the previous reports (Morinha et al 2012). Although PCR based gender determination techniques have several advantages over other techniques, always it demands purified DNA template without any PCR inhibitors for optimal result (Schrader et al 2012).…”

Section: Cc-by-nc-nd 40 International License Peer-reviewed) Is the mentioning

confidence: 99%

Gender identification in Chicken (Gallus gallus) by PCR using whole blood and dried blood spot on filter paper as template: without prior DNA isolation

Dhanasekaran¹,

G²,

Ar³

et al. 2016

Preprint

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Abstract:Accurate sex identification of pure line chickens in their early age has significant economic impact in breeding industry. In the recent years, range of Polymerase Chain Reaction (PCR) based sex determination techniques are routinely used to identify the sex of parent lines in breeding industries, however purified DNA is a prerequisite. Hence this study was aimed to develop a rapid and inexpensive PCR based gender identification method for chicken using whole blood samples and dried blood spots as template for PCR without DNA extraction. In addition, practicability of two W-chromosome specific gene targets in chicken for sex determination also characterised. Successful amplification of sex specific fragments and an internal control was achieved with the range of 0.125μl and 0.250μl volume of whole blood on filter paper (~1 mm) prepared from chicken and dried blood spot. This technique does not require DNA extraction, freeze/thawing of blood samples, pre-treatment with any reagents, dilution of whole blood or dried blood spots on filter paper. It can be carried out with commercially available Taq polymerase enzymes with increased concentration of MgCl 2 (3 mM) and 0.5% of DMSO without optimisation of PCR buffers. In conclusion, as compared to the existing PCR based sex identification techniques, the present approach is relatively economic, time saving, requires minimal steps and eliminates the need for DNA extraction. Introduction:

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Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods

Cited by 105 publications

References 109 publications

Gender identification using acoustic analysis in birds without external sexual dimorphism

Gender identification using acoustic analysis in birds without external sexual dimorphism

Using PCR-RFLP for sexing of the endangered Galápagos petrel (Pterodroma phaeopygia)

Gender identification in Chicken (Gallus gallus) by PCR using whole blood and dried blood spot on filter paper as template: without prior DNA isolation

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