Molecular serological characteristics of weak D antigen types of the Rhesus system

Головкина, Л. Л.; Ag, Stremoukhova; Td, Pushkina; Rs, Kalandarov; Gv, Atroshchenko; Mn, Vasil'eva; Vl, Surin; Salomashkina, V. V.; Pshenichnikova, O. S.; Gy, Miterev; Паровичникова, Е Н; Vg, Savchenko

doi:10.17116/terarkh201688778-83

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…Distribution of these alleles has been poorly studied so far. While RHD*weak D type 1.1 was initially thought to be restricted to a North‐Western area of Germany (Doescher et al, ), it was identified in Denmark and Russia (Christiansen et al, ; Golovkina et al, ), and more recently in 3/100 weak D samples in Australia (McGowan et al, ). These observations suggest that extended genotyping of the whole gene sequence may result in the identification of subtypes.…”

Section: Alleles and Genotyping Datamentioning

confidence: 99%

Weak D type 1, 2 and 3 subtype alleles are rare in the Western French population

Raud

Maréchal

Férec

et al. 2017

Transfusion Medicine

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Section: Alleles and Genotyping Datamentioning

confidence: 99%

Weak D type 1, 2 and 3 subtype alleles are rare in the Western French population

Raud

Maréchal

Férec

et al. 2017

Transfusion Medicine

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“…В России молекулярно-генетические методы при иммуногематологических исследованиях используют редко. Публикации с описанием случаев диагностики данных вариантов антигена немногочисленны [2,3,[11][12][13].…”

Section: актуальностьunclassified

Using the method of molecular genetic typing to determine variants of the weak antigen D in the diagnosis of Rh-affiliation

et al. 2023

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Background. Determination of Rh-affiliation is mandatory for donors and recipients, surgical patients, pregnant women, etc. There are variants of the D antigen that are difficult to identify by serological methods, for example, a weak D antigen. Aim. Determination of Rh-affiliation using genotyping in difficult cases, when the use of serological methods does not allow obtaining a reliable result. Material and methods. We studied blood samples from donors (n=18), pregnant women (n=17) and patients with hematological diseases (n=15): 22 men and 28 women, median age 36 years (25 to 54 years). Serologically, antigen D was determined by gel technology in ID-cards and in an indirect antiglobulin test with anti-D-IgG reagent. Weak D antigen variants were diagnosed and phenotype determined using polymerase chain reaction with allele-specific primers. For significance of differences in the frequency of types of weak antigen D, a nonparametric statistical method using a two-tailed Fisher's exact test was used. Differences were considered statistically significant at p 0.05. Results. The use of genotyping made it possible to detect the presence of a weak antigen D in 41 samples. Its specificity was represented by the following types: 1; 1.1; 2; 3. Other types of weak antigen D [4; 4.0; 4.1; 4.2 (DAR); 5; 11; 14; 15; 17] were absent. The study of the phenotype of erythrocyte antigens of the Rhesus system using genotyping revealed the predominance of the Ccee phenotype in people with a weak D antigen. Significant differences in the frequency of types of this antigen were revealed. Conclusion. The use of molecular genetic typing made it possible to determine the types of the weak antigen D and to accurately determine the Rh-affiliation of the subjects.

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Identification of common and new rare types of weak RhD antigen in patients with blood diseases and healthy person

et al. 2019

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Background. Rhesus phenotype has been determined in 404 persons which have problems with blood groups identification. Genetic typing of antigen RhD variants was performed in 73 individuals. Objective of the work was to give molecular and serological characteristics of the antigen RhD weak types.Materials and methods. Method of rhesus phenotype determination in direct agglutination test on plane by using of anti-D, anti-C, anti-c, anti-Cw, anti-E and anti-e monoclonal antibodies; gel method of rhesus phenotype determination; methods of genetic typing of RhD; methods of antigen RhD determination in the classic indirect antiglobulin test and in the gel indirect antiglobulin test; method of antigen RhD determination in the saline agglutination test.Results. Serological methods identified 73 red blood samples with the weakened expression of RhD antigen. Molecular methods showed the reasons of weakness of antigen expression. Three RHD*D weak types which are common in Russians (RHD*D weak type 1–3) were identified and for the first time 3 types were found – RHD*D weak type 67, RHD(G255R) and RHD(JVS5-38del4). Serological characteristic of RhD weak types was given. It was shown that combined using of monoclonal antibodies in direct agglutination test and in gel is the most effective serological method of the antigen variants detection. Red blood cells with weak RhD antigens can be recognized by weakness or absence of agglutination with monoclonal antibodies on plane if agglutination in gel was 3+4+.Conclusion. Concrete weak RhD variants can be determined only by genetic typing. Serologically weak antigen variants can be detected by using of at least two series of monoclonal antibodies or by using of two different methods (it is preferable).

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Molecular serological characteristics of weak D antigen types of the Rhesus system

Cited by 6 publications

References 21 publications

Weak D type 1, 2 and 3 subtype alleles are rare in the Western French population

Weak D type 1, 2 and 3 subtype alleles are rare in the Western French population

Using the method of molecular genetic typing to determine variants of the weak antigen D in the diagnosis of Rh-affiliation

Identification of common and new rare types of weak RhD antigen in patients with blood diseases and healthy person

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