Molecular screening of herbivorous flies collected from Hydrilla verticillata across China and Korea – setting up hypotheses for further exploratory surveys and tests

McCulloch, Graham A.; Purcell, Matthew F.; Harms, Nathan E.; Grodowitz, Michael J.; Zhang, Jialiang; Hong, Sun Hee; Walter, Gimme H.

doi:10.1016/j.biocontrol.2019.104051

Biological Control

2019

DOI: 10.1016/j.biocontrol.2019.104051

|View full text |Cite|

Molecular screening of herbivorous flies collected from Hydrilla verticillata across China and Korea – setting up hypotheses for further exploratory surveys and tests

Graham A. McCulloch

Matthew F. Purcell

Nathan E. Harms

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2019

2022

Publication Types

Select...

Article6

Relationship

Self Cite0

Independent6

Authors

Journals

Cited by 7 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the limits among species are very variable. Based on DNA barcode sequences, a clear intraspecific threshold was 2.5% for herbivorous fly identification [24], while that threshold was between 5.6-6.0% for dwelling water mite species identification [25]. Renaud et al (2012) assessed COI sequences of some species of Diptera and indicated the mean pairwise intraspecific distances (range 0.17-1.20%) and maximum intraspecific distances (range 3.00-5.40%) [26].…”

mentioning

confidence: 99%

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

Zheng

Zhang

Yang

et al. 2019

Insects

View full text Add to dashboard Cite

Tephritidae fruit flies (Diptera: Tephritidae) are regarded as important damage-causing species due to their ability to cause great economic losses in fruit and vegetable crops. Bactrocera minax and Bactrocera tsuneonis are two sibling species of the subgenus Tetradacus of Bactrocera that are distributed across a limited area of China, but have caused serious impacts. They share similar morphological characteristics. These characteristics can only be observed in the female adult individuals. The differences between them cannot be observed in preimaginal stages. Thus, it is difficult to distinguish them in preimaginal stages morphologically. In this study, we used molecular diagnostic methods based on cytochrome c oxidase subunit I and species-specific markers to identify these two species and improve upon the false-positive results of previous species-detection primers. DNA barcode sequences were obtained from 900 individuals of B. minax and 63 individuals of B. tsuneonis. Based on these 658 bp DNA barcode sequences of the cytochrome c oxidase subunit I gene, we successfully designed the species-specific primers for B. minax and B. tsuneonis. The size of the B. minax specific fragment was 422 bp and the size of the B. tsuneonis specific fragment was 456 bp. A series of PCR trials ensured the specificity of these two pairs of primers. Sensitivity assay results demonstrated that the detection limit for the DNA template concentration was 0.1~1 ng/µL for these two species. In this study, we established a more reliable, rapid, and low-cost molecular identification method for all life stages of B. minax and B. tsuneonis. Species-specific PCR can be applied in plant quarantine, monitoring and control of B. minax and B. tsuneonis.

show abstract

mentioning

confidence: 99%

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

Zheng

Zhang

Yang

et al. 2019

Insects

View full text Add to dashboard Cite

show abstract

The role of overseas genetic surveys to potentially accelerate biological control development for a new Hydrilla verticillata introduction in the USA

2020

View full text Add to dashboard Cite

The potential for biological control on cryptic plant invasions

Canavan

Harms³

et al. 2020

Biological Control

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Molecular screening of herbivorous flies collected from Hydrilla verticillata across China and Korea – setting up hypotheses for further exploratory surveys and tests

Cited by 7 publications

References 36 publications

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

The role of overseas genetic surveys to potentially accelerate biological control development for a new Hydrilla verticillata introduction in the USA

The potential for biological control on cryptic plant invasions

Contact Info

Product

Resources

About