Molecular <scp>HPA</scp> genotyping by microarray in <scp>B</scp>razilian blood donors

Conti, F; Bertrand, Gérald; Dezan, Marcia Regina; Costa, Thiago Henrique; Aravechia, Maria Giselda; Mota, Márcia Maria Peruzzi Elia da; Castilho, Lilian; Kaplan, C.; Kutner, José Mauro

doi:10.1111/trf.12272

Cited by 13 publications

(18 citation statements)

References 21 publications

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“…(; HPA‐1 to 5), a group of platelet donors studied by Conti et al . (; HPA‐1 to 5 and 15), and the sample blood donors analysed by Chiba et al . (; HPA‐1 to 4).…”

Section: Resultsmentioning

confidence: 99%

Human platelet antigen genotyping of platelet donors in southern Brazil

Merzoni

Fagundes

Lunardi

et al. 2015

Int J Immunogenetics

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Human platelet antigens (HPA) are immunogenic structures that result from single nucleotide polymorphisms (SNPs) leading to single amino acid substitutions. This study sought to determine the allele and genotype frequencies of HPA-1, HPA-2, HPA-3, HPA-4, HPA-5 and HPA-15 in platelet donors from the state of Rio Grande do Sul (RS), Brazil, and compare their allele frequencies to those observed in other populations. HPA genotyping was performed by PCR-SSP method. The study sample comprised 201 platelet donors (167 Caucasians and 34 non-Caucasians). Allele 'a' was that most commonly found for HPA-1 to 5 in both groups. The HPA-15ab genotype predominated over homozygous genotypes of this system. Fisher's exact test revealed statistically significant differences for the HPA-5 system, with a greater prevalence of the HPA-5b allele in non-Caucasians. The neighbour-joining method and principal components analysis revealed genetic proximity between our Caucasian group and European populations. We conclude that the allele frequencies of HPA-1 to 5 and HPA-15 found in our Caucasian sample are similar to those reported for European populations. These findings corroborate the ethnic makeup of the population of RS. The higher frequency of the HPA-5b allele found in the non-Caucasian group of our sample suggests the possibility of allosensitization in patients who receive platelet transfusions from genetically incompatible donors.

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“…(; HPA‐1 to 5), a group of platelet donors studied by Conti et al . (; HPA‐1 to 5 and 15), and the sample blood donors analysed by Chiba et al . (; HPA‐1 to 4).…”

Section: Resultsmentioning

confidence: 99%

Human platelet antigen genotyping of platelet donors in southern Brazil

Merzoni

Fagundes

Lunardi

et al. 2015

Int J Immunogenetics

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“…HPA‐3 frequencies described previously for São Paulo blood donors (Conti et al , ), Brazilian African descendants (Castro et al , ) and Slovenian and Swiss descendents (Boehlen et al , ) did not differ from results found in the Amazonas blood donors. This system was one of the most polymorphic systems found in this population, after HPA‐15, with 24·99% of mismatch probability.…”

Section: Discussionmentioning

confidence: 41%

“…It is related to the degree of variability in HPAs between donors and recipients, whose frequencies vary among different populations worldwide. Among the Brazilian population, considerable data on HPA prevalence is available for Caucasians, those of African descent and Amazonian Indians (Castro et al , ), as well as blood donors (Conti et al , ; Mangerona et al , ). However, the allele frequency data among Amazonian blood donors is still lacking.…”

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confidence: 99%

The human platelet alloantigen profile in blood donors from Amazonas, Brazil

Portela

Schriefer

Albuquerque

et al. 2016

Transfusion Medicine

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“…Platelet genotyping was performed using a BeadChip assay 17, 18. The BeadChip microarray method is capable of determining 22 allelic variants of 11 HPA systems (HPA-1 to HPA-9, HPA-11 and HPA-15).…”

Section: Methodsmentioning

confidence: 99%

Molecular typing of human platelet antigens in immune thrombocytopenia patients in northern Brazil

Carmo

Klippel

Cordeiro

et al. 2017

Revista Brasileira de Hematologia e Hemoterapia

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BackgroundImmune thrombocytopenia is an immune disease characterized by thrombocytopenia and bleeding due to platelet antibodies against platelet membrane glycoproteins. Human platelet antigens are derived from polymorphisms of these glycoproteins. The aim of this study was to investigate human platelet antigen frequencies in immune thrombocytopenia patients from the state of Amazonas, Brazil and investigate the potential association between specific antigens and risk for immune thrombocytopenia.MethodHuman platelet antigen typing was performed by BeadChip technology to determine allelic variants of 11 systems (HPA-1 to HPA-9, HPA-11 and HPA-15). Thirty-six patients (8 male and 28 female) with a median age of 34 years (range: 9–69 years) were evaluated and compared with data from Amazonas blood donors.ResultsPlatelet counts varied from 3 to 98 × 109/L. The allele frequencies were 0.944 for HPA-1a, 0.056 for HPA-1b, 0.847 for HPA-2a, 0.153 for HPA-2b, 0.555 for HPA-3a, 0.444 for HPA-3b, 0.805 for HPA-5a, 0.222 for HPA-5b, 0.9975 for HPA-9a, 0.025 for HPA-9b, 0.486 for HPA-15a and 0.513 for HPA-15b. Among immune thrombocytopenia individuals, no b allele of the HPA-4, -6, -7, -8 and -11 were found.ConclusionsThe results suggest HPA-1a, HPA-3b and HPA-5b are immune thrombocytopenia-specific autoepitopes.

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Molecular HPA genotyping by microarray in Brazilian blood donors

Cited by 13 publications

References 21 publications

Human platelet antigen genotyping of platelet donors in southern Brazil

Human platelet antigen genotyping of platelet donors in southern Brazil

The human platelet alloantigen profile in blood donors from Amazonas, Brazil

Molecular typing of human platelet antigens in immune thrombocytopenia patients in northern Brazil

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