Molecular response of Saccharomyces cerevisiae wine and laboratory strains to high sugar stress conditions

Jiménez‐Martí, Elena; Zuzuarregui, Aurora; Gomar-Alba, Mercè; Gutiérrez, Dolores; Gil, Concha; Olmo, Marcel·lí del

doi:10.1016/j.ijfoodmicro.2010.12.023

Cited by 46 publications

(38 citation statements)

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“…Increasing carbon and nitrogen levels only improves lipogenesis to a certain extent, as lipid yield decreases when glucose levels are increased to 160 g l À 1 ( Fig. 2c), and cultivation of various engineered strains in media containing 320 g l À 1 glucose drastically reduced growth rate, most likely due to osmotic stress 38 . Similar linkages between high carbon content versus caloric restriction may present interesting parallels in other organisms.…”

Section: Resultsmentioning

confidence: 99%

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

et al. 2014

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Economic feasibility of biosynthetic fuel and chemical production hinges upon harnessing metabolism to achieve high titre and yield. Here we report a thorough genotypic and phenotypic optimization of an oleaginous organism to create a strain with significant lipogenesis capability. Specifically, we rewire Yarrowia lipolytica's native metabolism for superior de novo lipogenesis by coupling combinatorial multiplexing of lipogenesis targets with phenotypic induction. We further complete direct conversion of lipid content into biodiesel. Tri-level metabolic control results in saturated cells containing upwards of 90% lipid content and titres exceeding 25 g l À 1 lipids, which represents a 60-fold improvement over parental strain and conditions. Through this rewiring effort, we advance fundamental understanding of lipogenesis, demonstrate non-canonical environmental and intracellular stimuli and uncouple lipogenesis from nitrogen starvation. The high titres and carbon-source independent nature of this lipogenesis in Y. lipolytica highlight the potential of this organism as a platform for efficient oleochemical production.

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Section: Resultsmentioning

confidence: 99%

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

et al. 2014

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“…Only at the highest concentration was HOG phosphorylated in P. nordicum indicating that glucose induces HOG phosphorylation much less that NaCl in both species. That is in contrast to the situation in Saccharomyces cerevisiae [30], suggesting that the influence of glucose in filamentous fungi might be different.…”

Section: Discussionmentioning

confidence: 64%

“…Hybridization was accomplished under stringent conditions as described by Sambrook and Russel [30]). The 1621 bp hog was labeled in a PCR reaction using the primers HOG_Pdigitatum_kpl_for (5'-ATGGCGGAATTCGTGCGTG-3') and HOG_Pdigitatum_kpl_rev (5'-TTATGCGAATGCTTGTCCAGTAGC-3').…”

Section: Methodsmentioning

confidence: 99%

Differences in the Regulation of Ochratoxin A by the HOG Pathway in Penicillium and Aspergillus in Response to High Osmolar Environments

Stoll

Schmidt‐Heydt

Geisen

2013

Toxins

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Penicillium verrucosum, P. nordicum and Aspergillus carbonarius are three important ochratoxin A producing species. P. verrucosum is in addition able to produce citrinin. It has been shown earlier that P. nordicum is adapted to NaCl rich environments like salt rich dry cured foods or even salines. In this organism, the biosynthesis of ochratoxin A plays an adaptive role in this habitat. P. verrucosum generally can be found on cereals, but occasionally also on salt rich dry cured foods. In contrast A. carbonarius usually cannot be found in NaCl rich environments, but it occurs in another environment with high concentration of solutes, e.g., in sugar rich substrates like grapes and grape juices. Usually osmotic challenging conditions activate the HOG MAP kinase signal cascade, which in turn activates various osmo-regulated genes. In the current analysis, it could be demonstrated that in case of P. nordicum and P. verrucosum the NaCl induced production of ochratoxin A is correlated to the phosphorylation status of the HOG MAP kinase. Just the opposite was true for A. carbonarius. In this case, also higher amounts of NaCl in the medium lead to an increased phosphorylation status of HOG, but no increase in ochratoxin biosynthesis was observed. In contrast to the Penicillia, higher NaCl concentrations lead to a rapid cessation of growth by A. carbonarius. High glucose concentrations have much less impact on growth and the phosphorylation of HOG.

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“…Several transcriptomic analyses have been carried out to understand the particularities of the response to this form of stress [13-15]. All these studies have indicated that three groups of genes display higher expression levels under these conditions: i) genes involved in glycerol metabolism, ii) genes participating in response to chemical stimulus, and iii) several genes of unknown function.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of this gene also increases under other hyperosmotic stress conditions (salt and sorbitol) and in response to other adverse conditions such as heat shock, oxidative damage produced by H 2 O 2 or diamide, ethanol, acid or basic pHs and the stationary phase [2,11,16]. The increase in the YHR087W mRNA levels under 20% glucose is also followed by higher content of the corresponding protein [15]. …”

Section: Introductionmentioning

confidence: 99%

The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress

2012

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BackgroundWhile growing in natural environments yeasts can be affected by osmotic stress provoked by high glucose concentrations. The response to this adverse condition requires the HOG pathway and involves transcriptional and posttranscriptional mechanisms initiated by the phosphorylation of this protein, its translocation to the nucleus and activation of transcription factors. One of the genes induced to respond to this injury is YHR087W. It encodes for a protein structurally similar to the N-terminal region of human SBDS whose expression is also induced under other forms of stress and whose deletion determines growth defects at high glucose concentrations.ResultsIn this work we show that YHR087W expression is regulated by several transcription factors depending on the particular stress condition, and Hot1p is particularly relevant for the induction at high glucose concentrations. In this situation, Hot1p, together to Sko1p, binds to YHR087W promoter in a Hog1p-dependent manner. Several evidences obtained indicate Yhr087wp’s role in translation. Firstly, and according to TAP purification experiments, it interacts with proteins involved in translation initiation. Besides, its deletion mutant shows growth defects in the presence of translation inhibitors and displays a slightly slower translation recovery after applying high glucose stress than the wild type strain. Analyses of the association of mRNAs to polysome fractions reveals a lower translation in the mutant strain of the mRNAs corresponding to genes GPD1, HSP78 and HSP104.ConclusionsThe data demonstrates that expression of Yhr087wp under high glucose concentration is controlled by Hot1p and Sko1p transcription factors, which bind to its promoter. Yhr087wp has a role in translation, maybe in the control of the synthesis of several stress response proteins, which could explain the lower levels of some of these proteins found in previous proteomic analyses and the growth defects of the deletion strain.

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Molecular response of Saccharomyces cerevisiae wine and laboratory strains to high sugar stress conditions

Cited by 46 publications

References 46 publications

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

Differences in the Regulation of Ochratoxin A by the HOG Pathway in Penicillium and Aspergillus in Response to High Osmolar Environments

The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress

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