Molecular requirements for attachment of the glycosylphosphatidylinositol anchor to the human alpha folate receptor

Tomassetti, Antonella; Bottero, Federica; Mazzi, Mimma; Miotti, Silvia; Colnaghi, Maria I.; Canevari, Silvana

doi:10.1002/(sici)1097-4644(19990101)72:1<111::aid-jcb12>3.0.co;2-1

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…Orr and Kamen [1995] described a nonfunctional mutant with a Ser3Pro substitution at position 67, which was completely unable to bind FA. We recently reported that the region spanning amino acids 233±237 is required for GPI processing of the receptor and anchoring to the membrane [Tomassetti et al, 1999]. The eight missense mutations detected in the CABA I aFR ORF in the present study appear to be distributed along the sequence with a slight preferential localization in the N-and the C-termini of the protein.…”

Section: Discussionsupporting

confidence: 48%

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Functional effect of point mutations in the ?-folate receptor gene of CABA I ovarian carcinoma cells

et al. 2001

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The alpha-folate receptor (alpha FR) is overexpressed in 90% of nonmucinous ovarian carcinomas. In addition to the known role of alpha FR binding and mediating the internalization of folates, functional interaction of alpha FR with signaling molecules was recently shown. To identify a model to study the role of alpha FR in ovarian carcinoma, we characterized the alpha FR gene in the ovarian carcinoma cell line CABA I in comparison to a reference line, IGROV1. In CABA I cells, Northern blot analysis revealed an alpha FR transcript of the expected length and FACS analysis indicated receptor expression on the cell membrane; however, RNase protection assay revealed no specific signals. Southern blot and genomic PCR analysis suggested the presence of a rearrangement(s) involving the 5' region of the gene in CABA I cells as compared to IGROV1 cells. Cloning and sequencing of CABA I alpha FR cDNA revealed several point mutations. The partitioning of alpha FR in membrane microdomains from CABA I cells and its association with regulatory molecules was comparable to that of IGROV1 cells. By contrast, the alpha FR expressed on the CABA I cell membrane bound folic acid with lower affinity, and ectopic expression of the corresponding cDNA in CHO cells confirmed impaired folic acid binding. Thus, CABA I cells may provide a tool to delineate functional domains of the alpha FR.

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Section: Discussionsupporting

confidence: 48%

“…CHO cells were transfected using the Lipofectin method as described [Tomassetti et al, 1999] with the pCR3.1 plasmid containing the CABA I or IGROV1 aFR cDNA. On Day 3, G418 (800 mg/ml) was added to the medium to select transfected cells.…”

Section: Cdna Stable Transfection In Cho Cellsmentioning

confidence: 99%

Functional effect of point mutations in the ?-folate receptor gene of CABA I ovarian carcinoma cells

et al. 2001

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“…Затем моноглутаматные формы превращаются в полиглутаматы. Фолатные рецепторы -богатые цистеином, заякоренные с по-мощью глюкозил-фосфатидилхолина мембранные гликопротеины, имеющие важное значение для пере-носа в клетку как фолатов, так и антифолатов [47].…”

Section: фолатные рецепторыunclassified

Possible biochemical mechanisms involved in beneficial and adverse effects of folates

et al. 2016

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О б з о р ы л и т е р а т у р ы Sola dosis facit venenum. Все есть яд, и все есть лекарство. Важна лишь доза.Парацельс.Ф олиевая кислота (витамин В 9 ) состоит из 2-амино-4-гидроксиптеридина (птерин), связанного с пара-аминобензойной кисло-той и остатком одной (в случае фолимоноглутамата) или более (в случае фолиполиглутамата) молекул глу-тамата. Различные коферментные формы витамина во-влечены в метаболизм одноуглеродных остатков. ИсточникиФолиевую кислоту из натуральных продуктов принято называть фолатом. Хорошим источником фолата счита-ются чечевица (358 мкг/чашка), репа (170 мкг/чашка), спаржа (268 мкг/чашка), брокколи (168 мкг/чашка), свекла (136 мкг/чашка), тыква (36 мкг/чашка), фасоль (230-294 мкг/чашка), арахис (351 мкг/чашка), а также говяжья печень (72 мкг/унция), яичный желток (22 мкг), молоко (12 мкг/чашка) и т.д. Фолиевая кислота синтези-руется также кишечными бактериями и транспортиру-ется специальным переносчиком.Ереванский Государственный медицинский университет им. Мхитара Гераци, Армения, г. Ереван Шалджян А.Л., Вартанян Г.С.*, Саарян А.В., Агаджанов М.И.В некоторых странах существует практика обогащения муки и мучных изделий фолиевой кислотой с целью снижения риска развития дефектов нервной трубки и анемии. Целью данного обзора является обсуждение целесообразности принудительного использования этих добавок. Установлено, что фолиевая кислота предотвращает развитие дефектов нервной трубки, в частности, spina bifida и anencephaly, а также препятствует развитию макроцитарной (мегалобластической) анемии. Способность фолиевой кислоты снижать уровень гомоцистеина лежит в основе ее протекторного воздействия на сердечно-сосудистую патологию. Однако высокий фолатный статус может подавлять ответ на антифолаты. Более того, сочетание высокой концентрации фолата с дефицитом витамина В 12 зачастую ассоциируется с повышением риска нарушения когнитивной функции и анемии. Согласно имеющейся информации, фолат оказывает двойственное влияние на канцерогенез, с одной стороны, препят-ствуя инициации, с другой -способствуя росту неопластических клеток и их метастазированию. Представляет ли фо-лиевая кислота фактор риска для развития некоторых патологий или оказывает благотворное действие, еще предстоит выяснить. Ключевые слова: фолат, обогащение фолиевой кислотой, гомоцистеин, дефекты нервной трубки (NTD).

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Addition of a Glycophosphatidylinositol to Acetylcholinesterase

Coussen

Ayon

Goff

et al. 2001

Journal of Biological Chemistry

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We introduced various mutations and modifications in the GPI anchoring signal of rat acetylcholinesterase (AChE). 1) The resulting mutants, expressed in transiently transfected COS cells, were initially produced at the same rate, in an active form, but the fraction of GPI-anchored AChE and the steady state level of AChE activity varied over a wide range. 2) Productive interaction with the GPI addition machinery led to GPI anchoring, secretion of uncleaved protein, and secretion of a cleaved protein, in variable proportions. Unproductive interaction led to degradation; poorly processed molecules were degraded rather than retained intracellularly or secreted. 3) An efficient glypiation appeared necessary but not sufficient for a high level of secretion; the cleaved, secreted protein was possibly generated as a by-product of transamidation. 4) Glypiation was influenced by a wider context than the triplet / ؉ 1/ ؉ 2, particularly ؊ 1. 5) Glypiation was not affected by the closeness of the site to the ␣ 10 helix of the catalytic domain. 6) A cysteine could simultaneously form a disulfide bond and serve as an site; however, there was a mutual interference between glypiation and the formation of an intercatenary disulfide bond, at a short distance upstream of . 7) Glypiation was not affected by the presence of an N-glycosylation site at or in its vicinity or by the addition of a short hydrophilic, highly charged peptide (FLAG; DYKDDDDK) at the C terminus of the hydrophobic region.

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Molecular requirements for attachment of the glycosylphosphatidylinositol anchor to the human alpha folate receptor

Cited by 4 publications

References 26 publications

Functional effect of point mutations in the ?-folate receptor gene of CABA I ovarian carcinoma cells

Functional effect of point mutations in the ?-folate receptor gene of CABA I ovarian carcinoma cells

Possible biochemical mechanisms involved in beneficial and adverse effects of folates

Addition of a Glycophosphatidylinositol to Acetylcholinesterase

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