Molecular reporters for CRISPR/Cas: From design principles to engineering for bioanalytical and diagnostic applications

Sohail, Muhammad; Qin, Lingyun; Li, Shuxian; Chen, Yue; Zaman, Muhammad Haidar; Zhang, Xing; Li, Bingzhi; Huang, He

doi:10.1016/j.trac.2022.116539

Cited by 23 publications

(10 citation statements)

References 167 publications

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“…ssRNA) in solution. In particular, the products of trans -cleavage can be detected and quantified using reporter molecules 105 (also called “reporters” or “substrate”) whose physico-chemical properties change upon cleavage. There exists a wide variety of reporters and associated means of detection, and so we review here reporter molecules and their associated detectors.…”

Section: Trans-cleavage and Detectionmentioning

confidence: 99%

A critical review of microfluidic systems for CRISPR assays

Avaro

Santiago

2023

Lab Chip

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Section: Trans-cleavage and Detectionmentioning

confidence: 99%

A critical review of microfluidic systems for CRISPR assays

Avaro

Santiago

2023

Lab Chip

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“…Encouraged by their unique biorecognition characteristics and superior cleaving ability, CRISPR/Cas systems have been widely used for the construction of CRISPR/Cas systems‐based biosensing technologies by integrating with the different signal sensing modes (e.g., colorimetric, fluorescence, and electrochemical modes) (Tang et al., 2021 ; Wan et al., 2022 ). By now, CRISPR/Cas systems‐based biosensing technologies have achieved an extensive application in multiple fields, such as disease diagnosis and nucleic acid detection due to their high speed, portability, high specificity and sensitivity, as well as no requirements for complex devices and well‐trained operators (Habimana et al., 2022 ; Phan, Truong, Medina‐Cruz, Dincer, & Mostafavi, 2022 ; Sohail et al., 2022 ). Recently, CRISPR/Cas systems‐based biosensing technologies have also been successfully applied in the highly sensitive and specific SARS‐CoV‐2 diagnosis in the ongoing COVID‐19 pandemic (Nouri, Tang, et al., 2021 ; Rahimi et al., 2021 ).…”

Section: Crispr/cas Systems‐based Sars‐cov‐2 Detectionmentioning

confidence: 99%

Point‐of‐care CRISPR/Cas biosensing technology: A promising tool for preventing the possible COVID‐19 resurgence caused by contaminated cold‐chain food and packaging

et al. 2022

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The ongoing coronavirus disease 2019 (COVID‐19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has caused great public health concern and has been a global threat due to its high transmissibility and morbidity. Although the SARS‐CoV‐2 transmission mainly relies on the person‐to‐person route through the respiratory droplets, the possible transmission through the contaminated cold‐chain food and packaging to humans has raised widespread concerns. This review discussed the possibility of SARS‐CoV‐2 transmission via the contaminated cold‐chain food and packaging by tracing the occurrence, the survival of SARS‐CoV‐2 in the contaminated cold‐chain food and packaging, as well as the transmission and outbreaks related to the contaminated cold‐chain food and packaging. Rapid, accurate, and reliable diagnostics of SARS‐CoV‐2 is of great importance for preventing and controlling the COVID‐19 resurgence. Therefore, we summarized the recent advances on the emerging clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system‐based biosensing technology that is promising and powerful for preventing the possible COVID‐19 resurgence caused by the contaminated cold‐chain food and packaging during the COVID‐19 pandemic, including CRISPR/Cas system‐based biosensors and their integration with portable devices (e.g., smartphone, lateral flow assays, microfluidic chips, and nanopores). Impressively, this review not only provided an insight on the possibility of SARS‐CoV‐2 transmission through the food supply chain, but also proposed the future opportunities and challenges on the development of CRISPR/Cas system‐based detection methods for the diagnosis of SARS‐CoV‐2.

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“…CRISPR technology has been incorporated into the detection of nucleic acid, possessing excellent simplicity, specificity, and sensitivity that surpasses conventional assays. − The Cas12a nuclease recognizes DNA and gets activated, thereby trans -cleaving nearby single-stranded DNA . This unique activity has been employed for the sensitive and precise in vitro detection of virus genomes, including SHERLOCK, DETECTOR, and HOLMES, ,, providing potent diagnostic toolboxes for point-of-care testing (POCT). , Aiming to control the COVID-19 pandemic, several CRISPR-based POCT targeting SARS-CoV-2 RNA was developed, − revealing high potential in-home tests. However, despite its potential, CRISPR-based POCT cannot replace the dominant role of conventional reverse transcription polymerase chain reaction (RT-PCR) in practical applications, mainly due to high bias.…”

Section: Introductionmentioning

confidence: 99%

Active CRISPR-Cas12a on Hydrophilic Metal–Organic Frameworks: A Nanobiocomposite with High Stability and Activity for Nucleic Acid Detection

Zeng

Shang

et al. 2023

Anal. Chem.

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CRISPR-Cas12a is an accurate and responsive biosensing technique, but its limited stability has restricted its widespread applications. To address this, we propose a strategy using metal−organic frameworks (MOFs) to protect Cas12a from harsh environments. After screening multiple candidate MOFs, it was found that hydrophilic MAF-7 is highly compatible with Cas12a, and the as-formed Cas12a-on-MAF-7 (COM) not only retains high enzymatic activity but also possesses excellent tolerance to heat, salt, and organic solvents. Further investigation showed that COM can serve as an analytical component for nucleic acid detection, resulting in an ultrasensitive assay for SARS-CoV-2 RNA detection with a detection limit of 1 copy. This is the first successful attempt to create an active Cas12a nanobiocomposite that functions as a biosensor without the need for shell deconstruction or enzyme release.

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Molecular reporters for CRISPR/Cas: From design principles to engineering for bioanalytical and diagnostic applications

Cited by 23 publications

References 167 publications

A critical review of microfluidic systems for CRISPR assays

A critical review of microfluidic systems for CRISPR assays

Point‐of‐care CRISPR/Cas biosensing technology: A promising tool for preventing the possible COVID‐19 resurgence caused by contaminated cold‐chain food and packaging

Active CRISPR-Cas12a on Hydrophilic Metal–Organic Frameworks: A Nanobiocomposite with High Stability and Activity for Nucleic Acid Detection

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