Molecular Relationships between Closely Related Species ofBothriocephalus(Cestoda: Platyhelminthes)

Verneau, Olivier; Catzeflis, François; Renaud, François

doi:10.1006/mpev.1996.0383

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…Although there is still no yardstick for recognizing genus boundaries by using DNA sequence differences, previous studies on the Pseudophyllidea (Verneau et al 1997;Kral'ova et al 2001) have shown the magnitude of interspecific and intergeneric nucleotide differences in the ITS region significantly exceeded the low level of the nucleotide variation between the Ligula and Digamma specimens observed in the present study. The 5¢ end of 28S rDNA for Digramma samples showed a high level of identity to those of Ligula, even in the rapidly evolving D1 domain of 28S rDNA.…”

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confidence: 64%

Is the genus Digramma synonymous to the genus Ligula (Cestoda: Pseudophyllidea)?

et al. 2003

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The genus Digramma (Cestoda: Pseudophyllidea) described by Cholodkovsky in 1915 differs from the genus Ligula only by the number of the reproductive organs per proglottis. However, the occurrence of transitional forms in Digramma raises much confusion concerning its generic validity. In the present study, cestodes previously designated as Digramma and Ligula were collected from lakes in the lower and middle reaches of the Yangtze River, and also from Qinghai Lake on Qingzang plateau, China. The entire internal transcribed spacer of the ribosomal DNA (ITS rDNA) and 5¢ end of 28S rDNA were compared between the Digramma and Ligula specimens. The low level of nucleotide variation between the two genera may imply that cestodes in the genus Digramma are paraphyletic to the Ligula genus, and Digramma is a synonym of Ligula. However, whether previously identified Digramma cestodes represent different species in the genus Ligula requires further investigation.The genus Digramma was erected by Cholodkovsky (1915) for Ligula alternans Rudolphi, 1810, an adult cestode of a gull Larus tridactylus in Europe. The only morphological difference considered to be of generic importance is the number of reproductive complexes per proglottis: one row of reproductive organs in Ligula, but two in Digramma. Although the reproductive organ is a relatively stable character, Wardle and Meleod (1952) speculated that Digramma was merely an uncommon diplogonadic morph of Ligula Bloch, 1782. Dubinina (1980) reported sexual dimorphism in Digramma. In bream (Abramis brama L.) they are found with reproductive organs in one row, whereas two rows occur in those from carp (Cyprinus carpio L.) and goldfish [Carassius auratus (L.)] over the entire length of the worm. In the same locality, Tai Hu lake on the lower and middle reaches of the Yangtze River, Liao and Liang (1987) found that plerocercoids in fish of the family Salangidae possess only a single row of reproductive complexes, but two regular rows are always present in those from Hemiculter and Acanthorhodeus. Thus, much confusion remains on the status of the genus Digramma. On the other hand, since the erection of the genus, the tapeworm has only been reported from cyprinid hosts in Russia and China (Dubinina 1980;Liao and Liang 1987). Studies on this cestode have been related to its geographical distribution and the pathological effect on its fish hosts (Evlanov and Kolokol'nikova 1990;Krylov et al. 1993), but little has been done on the redescription or validity of the genus. The objective of this study was to compare the phylogenetic differences in sequences of the entire internal transcribed spacer of the ribosomal DNA (ITS rDNA) and 5¢ end of 28S rDNA between Digramma and Ligula groups, and to determine whether the differences vary at the intergeneric and/or intra-and interspecific levels.Plerocercoids of Digramma and Ligula were collected from the body cavities of cyprinid fish from lakes on the lower and middle reaches of the Yangtze River in Hubei, Jiangxi provinces, China and also fro...

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confidence: 64%

Is the genus Digramma synonymous to the genus Ligula (Cestoda: Pseudophyllidea)?

et al. 2003

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“…In a series of studies on the evolutionary relationship of morphologically very similar seven species of Bothriocephalus which infest a large spectrum of marine teleost fish (Pleuronectiformes, Scorpaeniformes and Gadiformes), Verneau et al (1997aVerneau et al ( , 1997b showed that the parasites may not have coevolved with their hosts, although co-evolution has been the most parsimonious explanation for hostparasite assemblages (Brooks, 1979). They interpreted the parasite-host relationship as two different, but non-exclusive, evolutionary processes: (1) sequential colonisations of different host species followed by speciation of the parasites; and (2) a rapid radiation of a ubiquitous ancestral parasite species, leading to the isolation and differentiation of Bothriocephalus lineages in the different host species.…”

Section: Discussionmentioning

confidence: 99%

Molecular variation of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) in different fish host species based on ITS rDNA sequences

et al. 2002

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The molecular variation in Bothriocephalus acheilognathi Yamaguti, 1934 from 11 species of freshwater fish collected in Australia, China, the Czech Republic, England and Hawaii was investigated by determining the nucleotide sequences of the internal transcribed spacer region. The length of the first and second internal transcribed spacer sequences of multiple individuals ranged from 553 to 571 bp and 553 to 615 bp, and the G + C content from 53.1 to 53.5%. The percentage sequence divergence varied between 0 and 0.9% in the ITS1 and 0 and 6.6% in the ITS2, respectively, indicating the occurrence of intraspecific variation. It is demonstrated that the fragment length variation resulted primarily from microsatellite polymorphisms present in the ITS region, especially in the ITS2 region. Phylogenetic analyses revealed that B. acheilognathi examined in this study consisted of three closely related genotypes with certain degrees of host-specificity, and the genotype representing isolates from Cyprinus carpio L. was the most common and diverse form within the species B. acheilognathi.

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Comparative karyological analysis of three species of Bothriocephalus Rudolphi 1808 (Cestoda: Pseudophyllidea)

Petkevičiūtė¹

2003

Parasitol Res

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The diploid complements of mitotic metaphase plates of three species of Botriocephalus were studied using conventional Giemsa staining and karyometric analysis. Botriocephalus claviceps and Botriocephalus gregarius showed a karyotype with 2n=14 chromosomes. All chromosomes of B. claviceps were biarmed, metacentric or meta-submetacentric. The two first pairs of homologues were markedly larger than the remaining elements and represented 48.62% of the total chromosome length. The karyotype of B. gregarius had similar metric values for the chromosomes, but clearly differed in the chromosome morphology of pairs 5, 6 and 7, which had terminally located centromeres. The diploid number 2n=12 was found in Botriocephalus scorpii. The karyotype consists of five pairs of metacentric and one pair of submeta-metacentric chromosomes. The possible pathways of karyotype evolution within Bothriocephalus spp. and their phylogenetic relations with the other karyologically studied groups in the order Pseudophyllidea are discussed.

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Molecular Relationships between Closely Related Species ofBothriocephalus(Cestoda: Platyhelminthes)

Cited by 5 publications

References 48 publications

Is the genus Digramma synonymous to the genus Ligula (Cestoda: Pseudophyllidea)?

Is the genus Digramma synonymous to the genus Ligula (Cestoda: Pseudophyllidea)?

Molecular variation of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) in different fish host species based on ITS rDNA sequences

Comparative karyological analysis of three species of Bothriocephalus Rudolphi 1808 (Cestoda: Pseudophyllidea)

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