Molecular regulation of plant somatic embryogenesis

Elhiti, Mohamed; Stasolla, Claudio; Wang, Aiming

doi:10.1007/s11627-013-9547-3

Cited by 143 publications

(104 citation statements)

References 85 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…Somatic embryogenesis (SE) is an important pathway for the regeneration of plants, as well as an attractive system for the study of the morphology, physiology, and genetic mechanisms of embryo induction and development (Elhiti et al 2013;Fehér 2015). Although this process was first described many years ago, our present knowledge about the acquisition of embryogenic competence by somatic cells remains unknown, and it is unexplored in ferns.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Grzyb

Kalandyk

Waligórski

et al. 2017

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

of explant excision, a dramatic reduction in concentration of all phytohormones studied was observed, but hormonal balance and sugar content remained almost unchanged. During the 14-day-long culture, the ABA/CKs and ABA/ IAA ratios remained constant, whereas the greatest differences were detected for the IAA/CKs and Z-type/iPA cytokinin ratios. Excluding day 6 of culture, cytokinins were found to be the predominant phytohormones over IAA. An almost 12-fold increase in soluble sucrose concentration at day 6 of culture might be the switch to the SE expression phase. Frequent cell divisions leading to somatic embryo formation are clearly associated with increase in trans-zeatin riboside content. Keywords

show abstract

Section: Introductionmentioning

confidence: 99%

“…Phytohormones are employed as signalling molecules for the induction of SE by mediating the signal transduction cascade leading to the reprogramming of gene expression patterns (Elhiti et al 2013). Some hormones, such as abscisic acid (ABA) and cytokinins have been shown to help regulate the sugar metabolism and transport.…”

Section: Introductionmentioning

confidence: 99%

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Grzyb

Kalandyk

Waligórski

et al. 2017

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…the lowest-cost methods for producing uniform, identical plants, particularly of those species that are difficult to propagate in a natural way or in other conventional manner. The process is also used to study the embryonic development regulation at morphological, biochemical, genetic and epigenetic levels (Willemsen and Scheres 2004;Elhiti et al 2013;Smertenko and Bozhkov 2014). Competence to somatic embryogenesis is highly correlated with the genotype.…”

Section: Introductionmentioning

confidence: 99%

Identification of LEC1, L1L and Polycomb Repressive Complex 2 genes and their expression during the induction phase of Medicago truncatula Gaertn. somatic embryogenesis

Orłowska

Igielski

Łagowska

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

However, the callus formation was observed in the nonembryogenic line as well, but the LEC1 and L1L genes were not expressed and the transcription of PRC2 genes was at stable level. It was only the SWN expression that decreased at the beginning of induction and did not change in the subsequent days in both lines. Our results indicate that LEC1 and L1L, known as marker genes of the late developmental stages, may be associated with the acquisition of embryogenic competency by somatic cells (prime events in the SE induction). The PRC2 complex genes are also expressed during embryogenic callus tissue formation on Medicago tuncatula leaf explants.

show abstract

“…Somatic embryogenesis is a type of plant totipotency in which embryos are induced to form on vegetative explants, usually in response to exogenous hormones, especially auxins, and/or stress treatments (Fehér, 2015). Somatic embryogenesis is used extensively as a clonal propagation tool in biotechnology applications (LeluWalter et al, 2013;Sharma et al, 2013b;Park and Paek, 2014), and while the tissue culture requirements for somatic embryo induction are well known (Gaj, 2004), only a few of the molecular components that drive this process have been described (Elhiti et al, 2013).…”

mentioning

confidence: 99%

The BABY BOOM Transcription Factor Activates the LEC1-ABI3-FUS3-LEC2 Network to Induce Somatic Embryogenesis

et al. 2017

View full text Add to dashboard Cite

Somatic embryogenesis is an example of induced cellular totipotency, where embryos develop from vegetative cells rather than from gamete fusion. Somatic embryogenesis can be induced in vitro by exposing explants to growth regulators and/or stress treatments. The BABY BOOM (BBM) and LEAFY COTYLEDON1 (LEC1) and LEC2 transcription factors are key regulators of plant cell totipotency, as ectopic overexpression of either transcription factor induces somatic embryo formation from Arabidopsis (Arabidopsis thaliana) seedlings without exogenous growth regulators or stress treatments. Although LEC and BBM proteins regulate the same developmental process, it is not known whether they function in the same molecular pathway. We show that BBM transcriptionally regulates LEC1 and LEC2, as well as the two other LAFL genes, FUSCA3 (FUS3) and ABSCISIC ACID INSENSITIVE3 (ABI3). LEC2 and ABI3 quantitatively regulate BBM-mediated somatic embryogenesis, while FUS3 and LEC1 are essential for this process. BBM-mediated somatic embryogenesis is dose and context dependent, and the contextdependent phenotypes are associated with differential LAFL expression. We also uncover functional redundancy for somatic embryogenesis among other Arabidopsis BBM-like proteins and show that one of these proteins, PLETHORA2, also regulates LAFL gene expression. Our data place BBM upstream of other major regulators of plant embryo identity and totipotency.

show abstract

Molecular regulation of plant somatic embryogenesis

Cited by 143 publications

References 85 publications

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Identification of LEC1, L1L and Polycomb Repressive Complex 2 genes and their expression during the induction phase of Medicago truncatula Gaertn. somatic embryogenesis

The BABY BOOM Transcription Factor Activates the LEC1-ABI3-FUS3-LEC2 Network to Induce Somatic Embryogenesis

Contact Info

Product

Resources

About