Molecular Recombination in the Ligase Negative T4 Amber Mutant

Kozinski, Andrzej W.

doi:10.1101/sqb.1968.033.01.044

Cited by 36 publications

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“…Polynucleotide ligases have been purified from both uninfected (12,13,31,40) and virus-infected Escherichia coli (1, 7, 37) and from mammalian tissues (26). These enzymes, which seal singlestranded breaks in duplex deoxyribonucleic acid (DNA), are thought to be involved in DNA repair and replication (10,14,21,24,29,30). In the present study, we have found high levels of ligase activity in several types of mammalian tissue culture cells.…”

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confidence: 54%

Polynucleotide Ligase Activity in Cells Infected with Simian Virus 40, Polyoma Virus, or Vaccinia Virus

Sambrook

Shatkin

1969

J Virol

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The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infection of mouse embryo, monkey kidney, and HeLa cells with polyoma virus, SV40, and vaccinia virus, respectively, the enzyme activity increased, but its cofactor requirement was unchanged. In vaccinia virus-infected cells, the increased activity was localized in the cytoplasm. Ligase induction occurred in the presence of cytosine arabinoside but was prevented by puromycin. Rifampicin blocked the production of infectious vaccinia particles but had little effect on the induction of ligase.

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confidence: 54%

Polynucleotide Ligase Activity in Cells Infected with Simian Virus 40, Polyoma Virus, or Vaccinia Virus

Sambrook

Shatkin

1969

J Virol

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“…"Fast-sedimenting complexes" between primary T2 (or T4) DNA and some cellular component (cytoplasmic membrane?) have been frequently observed (19,26,33).…”

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confidence: 97%

Breakdown and Exclusion of Superinfecting T-Even Bacteriophage in Escherichia coli

Anderson

Eigner

1971

J Virol

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In bacterial strains containing the deoxyribonuclease endonuclease I (endonuclease I + strains), 70 to 80% of the injected superinfecting T-even phage deoxyribonucleic acid (DNA) is rapidly degraded to oligonucleotides having an average chain length of 8, the same value as that obtained by endonuclease I digestion of purified T-even phage DNA in vitro. In endonuclease I − strains, less than 5% of the injected superinfecting T-even phage DNA is degraded to acid-soluble components. The superinfecting phage DNA is, however, fragmented into a large segment having a molecular weight of about 90 × 10 6 and 30 or more small acid-insoluble segments having molecular weights of less than 10 6 . In both endonuclease I + and endonuclease I − strains, over 80% of the DNA from adsorbed primary T2 or T4 phage, but only 50% of the DNA from adsorbed superinfecting T2 or T4 phage, is injected. Superinfecting T4 are genetically excluded as efficiently from endonuclease I − strains as they are from endonuclease I + strains. The excluded phage cannot complement defects in either early or late gene functions carried by the primary phage. The induction of both superinfection breakdown and superinfection exclusion requires a period of protein synthesis between primary infection and addition of the superinfecting phage. These observations seem best explained by failure of superinfecting DNA to enter the host cell cytoplasm, presumably as a result of changes in the cell envelope induced by the primary phage.

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“…However, failure of one of the processes, for example, process [3] or [4] of the possible recombination pathway, or an abnormal enhancement of the initial steps of process [1] or [2] would fail to give rise to recombinant molecules and may result in the accumulation of intermediate molecules of the recombination pathway. Huberman (1968), and Kozinski (1968) have observed T4 and 2DNA molecules containing branches which could have resulted from genetic recombination. Huberman (1968), and Kozinski (1968) have observed T4 and 2DNA molecules containing branches which could have resulted from genetic recombination.…”

Section: Introductionmentioning

confidence: 99%

Joint molecules of λ DNA as an intermediate of genetic recombination

Takahashi

1977

Molec. Gen. Genet.

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Joint molecules of lambda DNA formed in the absence of DNA replication, which may be involved in the process of genetic recombination can be observed as branched DNA derived from different phage particles. These molecules are associated through base-pair hydrogen bonding in synaptic regions, usually with short single-stranded gaps. Furthermore, joint molecules could be accumulated up to ten fold when lambda was irradiated with ultraviolet light before infection of polI mutant of E. coli. Infection at low multiplicity did not give rise to joint molecules. These results suggest that single-strand breaks and gaps introduced in duplex lambda DNA facilitate the formation of joint molecules.

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Molecular Recombination in the Ligase Negative T4 Amber Mutant

Cited by 36 publications

References 0 publications

Polynucleotide Ligase Activity in Cells Infected with Simian Virus 40, Polyoma Virus, or Vaccinia Virus

Polynucleotide Ligase Activity in Cells Infected with Simian Virus 40, Polyoma Virus, or Vaccinia Virus

Breakdown and Exclusion of Superinfecting T-Even Bacteriophage in Escherichia coli

Joint molecules of λ DNA as an intermediate of genetic recombination

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