Molecular recognition with nanostructures fabricated by photopolymerization within metallic subwavelength apertures

Urraca, Javier L.; Barrios, Carlos Angulo; Canalejas-Tejero, Víctor; Orellana, Guillermo; Moreno‐Bondi, María C.

doi:10.1039/c4nr01129e

Cited by 15 publications

(18 citation statements)

References 18 publications

(20 reference statements)

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“…Some molecules are intrinsically fluorescent because of their combined aromatic groups or other rigid plane structure with several conjugated double bonds (Miller et al, 2010). These fluorescent molecules can act as templates in common MIPs preparation, and output fluorescence signals including fluorescence intensity (Carrasco et al, 2014), polarization (Ton et al, 2012) and lifetime (Urraca et al, 2014) during the rebinding procedure for quantification ( Fig. 5A).…”

Section: Direct Constructionmentioning

confidence: 99%

“…Upon excitation by plane-polarized light, the tumbling rate of free excited enrofloxacin in solution was rapid so that the polarization value was negligible; inversely, enrofloxacin-MIP complex corresponded to lower tumbling rate but higher polarization value via polarized emission. Moreover, through fluorescence lifetime image technology with single photon timing measurements, Moreno-Bondi's group (Urraca et al, 2014) fabricated MIP patterns with submicron lateral resolution by photoinduced imprinting R123 within metallic subwavelength apertures, and the native fluorescence allowed sensitive detection of the binding events.…”

Section: Direct Constructionmentioning

confidence: 99%

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Strategies of molecular imprinting-based fluorescence sensors for chemical and biological analysis

Yang

Wang

et al. 2018

Biosensors and Bioelectronics

321

126

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One pressing concern today is to construct sensors that can withstand various disturbances for highly selective and sensitive detecting trace analytes in complicated samples. Molecularly imprinted polymers (MIPs) with tailor-made binding sites are preferred to be recognition elements in sensors for effective targets detection, and fluorescence measurement assists in highly sensitive detection and user-friendly control. Accordingly, molecular imprinting-based fluorescence sensors (MI-FL sensors) have attracted great research interest in many fields such as chemical and biological analysis. Herein, we comprehensively review the recent advances in MI-FL sensors construction and applications, giving insights on sensing principles and signal transduction mechanisms, focusing on general construction strategies for intrinsically fluorescent or nonfluorescent analytes and improvement strategies in sensing performance, particularly in sensitivity. Construction strategies are well overviewed, mainly including the traditional indirect methods of competitive binding against pre-bound fluorescent indicators, employment of fluorescent functional monomers and embedding of fluorescence substances, and novel rational designs of hierarchical architecture (core-shell/hollow and mesoporous structures), post-imprinting modification, and ratiometric fluorescence detection. Furthermore, MI-FL sensor based microdevices are discussed, involving micromotors, test strips and microfluidics, which are more portable for rapid point-of-care detection and in-field diagnosing. Finally, the current challenges and future perspectives of MI-FL sensors are proposed.

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Section: Direct Constructionmentioning

confidence: 99%

Section: Direct Constructionmentioning

confidence: 99%

Strategies of molecular imprinting-based fluorescence sensors for chemical and biological analysis

Yang

Wang

et al. 2018

Biosensors and Bioelectronics

321

126

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“…We have demonstrated a new technique for fabricating MIP patterns with sub-micrometer lateral resolution: Photoinduced local polymerization within Al nanoholes [ 46 ]. The fabrication procedure is schematically depicted in Figure 8 .…”

Section: Al Nanoholes For Nanomip Synthesismentioning

confidence: 99%

Aluminum Nanoholes for Optical Biosensing

et al. 2015

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Sub-wavelength diameter holes in thin metal layers can exhibit remarkable optical features that make them highly suitable for (bio)sensing applications. Either as efficient light scattering centers for surface plasmon excitation or metal-clad optical waveguides, they are able to form strongly localized optical fields that can effectively interact with biomolecules and/or nanoparticles on the nanoscale. As the metal of choice, aluminum exhibits good optical and electrical properties, is easy to manufacture and process and, unlike gold and silver, its low cost makes it very promising for commercial applications. However, aluminum has been scarcely used for biosensing purposes due to corrosion and pitting issues. In this short review, we show our recent achievements on aluminum nanohole platforms for (bio)sensing. These include a method to circumvent aluminum degradation—which has been successfully applied to the demonstration of aluminum nanohole array (NHA) immunosensors based on both, glass and polycarbonate compact discs supports—the use of aluminum nanoholes operating as optical waveguides for synthesizing submicron-sized molecularly imprinted polymers by local photopolymerization, and a technique for fabricating transferable aluminum NHAs onto flexible pressure-sensitive adhesive tapes, which could facilitate the development of a wearable technology based on aluminum NHAs.

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“…Nanoplasmonic sensors supporting SPR have been employed in various biosensing applications in the past [ 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. In terms of the design of POC devices, metallic nanostructures such as surface-relief gratings (SRGs) offer several key advantages including very small footprint, portability and compatibility with collinear optics, providing easiness of integration with other microsystems [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Selective Uropathogenic E. coli Detection Using Crossed Surface-Relief Gratings

Nair

Gomez-Cruz

Manjarrez-Hernández

et al. 2018

Sensors

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Urinary tract infections (UTIs) are one of the major burdens on public healthcare worldwide. One of the primary causes of UTIs is the invasion of the urinary tract by uropathogenic Escherichia coli (UPEC). Improper treatment of bacterial infections like UTIs with broad-spectrum antibiotics has contributed to the rise of antimicrobial resistance, necessitating the development of an inexpensive, rapid and accurate detection of UPEC. Here, we present real-time, selective and label-free detection of UPEC using crossed surface-relief gratings (CSRGs) as nanometallic sensors incorporated into an optical sensing platform. CSRGs enable real-time sensing due to their unique surface plasmon resonance (SPR)-based light energy exchange, resulting in detection of a very-narrow-bandwidth SPR signal after the elimination of residual incident light. The platform’s sensing ability is experimentally demonstrated by the detection of bulk refractive index (RI) changes, with a bulk sensitivity of 382.2 nm/RIU and a resolution in the order of 10−6 RIU. We also demonstrate, for the first time, CSRG-based real-time selective capture and detection of UPEC in phosphate-buffered saline (PBS) solution, in clinically relevant concentrations, as opposed to other UTI-causing Gram-negative bacteria. The platform’s detection limit is calculated to be 105 CFU/mL (concentration on par with the clinical threshold for UTI diagnosis), with a dynamic range spanning four orders of magnitude. This work paves the way for the development of inexpensive point-of-care diagnosis devices focusing on effective treatment of UTIs, which are a burden on public healthcare due to the rise in the number of cases and their recurrences in the recent past.

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Molecular recognition with nanostructures fabricated by photopolymerization within metallic subwavelength apertures

Cited by 15 publications

References 18 publications

Strategies of molecular imprinting-based fluorescence sensors for chemical and biological analysis

Strategies of molecular imprinting-based fluorescence sensors for chemical and biological analysis

Aluminum Nanoholes for Optical Biosensing

Selective Uropathogenic E. coli Detection Using Crossed Surface-Relief Gratings

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