Molecular Recognition of Avidin on Biotin-Functionalized Gold Surfaces Detected by FT-IRRAS and Use of Metal Carbonyl Probes

Synthesis, characterization, X-ray crystallography, and cytotoxicity of a cymantrene keto carboxylic acid for IR labelling of bioactive peptides on a solid support

“…We are particularly interested in the attachment of IR spectroscopic labels to peptides for biological imaging applications with scanning near-field IR microscopy (SNIM) [23]. Searching for marker groups with strong vibrational bands in a spectroscopic window not obscured by the signals of bio(macro)molecules, metal carbonyl complexes are an obvious choice [24]. Very recently, for example, Co 2 (CO) 6 -alkyne and TpRe(CO) 3 (Tp = tris(pyrazolyl)borate) peptide bioconjugates have been reported [25,18].…”

Microwave-assisted solid-phase synthesis, cellular uptake, and cytotoxicity studies of cymantrene–peptide bioconjugates

“…In addition, most of these functionalities were introduced by manual coupling. We thus became interested in cymantrene (CpMn(CO) 3 ) as an easy-to-functionalize and robust IR spectroscopic marker [25][26][27][28][29][30][31][32][33] and show here that microwave-assisted solid-phase synthesis allows one to prepare different cymantrene-peptide bioconjugates of increasing complexity in good yield and purity. [Leu 5 ]-enkephalin (ENK) was chosen as a simple pentapeptide with few charged amino acid residues to start with and validate the solid-phase peptide synthesis (SPPS) method.…”

Immobilization of atrazine on gold, a first step towards the elaboration of an indirect immunosensor: characterization by XPS and PM-IRRAS

“…8 Subsequent binding of the protein avidin was detected by IRRAS. 9,10 Under optimized conditions and in combination with metallo-carbonyl probes, the limit of detection of avidin in solution reached the nanomolar range. 11 It was also demonstrated that the density and order of the primary SAM to which biotin was grafted were of major importance for the sensor sensitivity.…”