Molecular recognition at self-assembled monolayers: Optimization of surface functionalization

Abstract

Articles you may be interested inUnderstanding the nonfouling mechanism of surfaces through molecular simulations of sugar-based selfassembled monolayers Nanostructural and electrical properties of functionally terminated self-assembled monolayers on silicon surfacesSulfur-based molecules containing biotin and hydroxyl groups have been used to create a wide variety of self-assembled monolayers on gold surfaces. Surface plasmon resonance has been used to study in situ the binding of streptavidin to these monolayers from solution. The self-assembled monolayers allow a high degree of control over the surface properties. The choice of an appropriate biotin-containing molecule, with a spacer segment, and the dilution of this molecule within the monolayer by hydroxythiols, allows optimization of the binding properties of the monolayer-nonspecific interactions between streptavidin and the surface are below detection limits, and specific binding between the streptavidin and biotin groups can be maximized. EXPERIMENTAL SECTION MaterialsPrisms and slides used were either LaSFN9, or BK7 Schott glass.Biotin was purchased from Sigma. Solvents were of Chromasolv quality from Riedel de Haen. All chemicals were used as received. Water was purified using an ion exchange purification train (MilliQ system, Millipore). Synthesis of thiols, disulfides, and sulfidesThe sulfur-based compounds used are shown in Fig. 1. Compounds 1 and 2 were synthesized following Ref. 18. Compound 3 is a byproduct of the synthesis and can be 7012

Preparation of Bio-Functionalized Surfaces with an Electrochemically Active Silane Presenting Concealed Aldehyde

“…From avidin immobilization study together with the XPS results, we presume the absolute density of the attached biotin is not large even under the acidic pH, because it is well known that the largest yield of avidin immobilization is found at one tenth of a full monolayer density of biotin. 13 The exclusive pattern result from highly acidic pH indicates that the attachment of biotin hydrazide is negligible on the other electrodes and glass surfaces presenting the protected aldehyde, which supports a high spatial selectivity of our immobilization method based on electrochemical addressing.…”

Carbonic Anhydrase as a Model for Biophysical and Physical-Organic Studies of Proteins and Protein−Ligand Binding

“…620 To explain the data, they invoked the concept of lateral steric effects [622][623][624][625][626][627] -an unfavorable steric repulsion between BCA II that is bound at the surface and BCA II that is free in solution ( Figure 10C; section 8.4.3). Decreasing the fractional coverage of arylsulfonamide ligands on the surface should reduce the possibility of these unfavorable interactions between bound BCA II molecules and increase the observed value of k on to its solution-phase value.…”

Effect of ionic strength on PNA-DNA hybridization on surfaces and in solution

“…35,36 11-Mercapto-1-undecanol, sodium chloride, monosodium phosphate ͑NaH 2 PO 4 ͒, disodium phosphate ͑Na 2 HPO 4 ͒, and absolute ethanol were purchased from Aldrich. The chemical structures and the sequences of the oligonucleotides used are summarized in Table I.…”