Molecular properties and thioredoxin‐mediated activation of spinach chloroplastic NADP‐malate dehydrogenase

Ferté, Nathalie; Meunier, Jean‐Claude; Ricard, Jacques; Buc, Jean; Sauve, Paul

doi:10.1016/0014-5793(82)80720-5

Cited by 20 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are similar discrepancies in the reported MW of spinach NADP-MDH. The inactive form of the enzyme from spinach chloroplasts is reported to have a MW of about 56,000 as determined by gel chromatography on Sephadex G-200 and equilibrium sedimentation, and a subunit MW of 28,000 (33). Earlier, Ting & Rocha (93) reported that the MW of the active spinach enzyme was 110,000 and that the inactive enzyme appeared as three peaks on Sephadex G-200 .…”

Section: Physical and Molecular Propertiesmentioning

confidence: 96%

Pyruvate,Pi Dikinase and NADP-Malate Dehydrogenase in C4 Photosynthesis: Properties and Mechanism of Light/Dark Regulation

Edwards¹,

Nakamoto²,

Burnell³

et al. 1985

Annu. Rev. Plant. Physiol.

196

View full text Add to dashboard Cite

Section: Physical and Molecular Propertiesmentioning

confidence: 96%

Pyruvate,Pi Dikinase and NADP-Malate Dehydrogenase in C4 Photosynthesis: Properties and Mechanism of Light/Dark Regulation

Edwards¹,

Nakamoto²,

Burnell³

et al. 1985

Annu. Rev. Plant. Physiol.

196

View full text Add to dashboard Cite

“…[34], using in vitro translation and immunoprecipitation. For C3 plants, reported monomer molecular weights for NADP-MDH have varied from 28 to 40 kDa (kD) [10,12], whereas the reported molecular weights for the C4 proteins range from 29 to 45 kDa [2,11,15,18,21]. Since the N-terminus of the mature protein has been established [ 8 ], the amino acid sequence derived from our eDNA nucleotide sequence predicts a molecular weight of 40 934 Da.…”

Section: K D T K W L E E E F T L T V Q K R G G V L I Q K W G R S S a mentioning

confidence: 99%

Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization

1989

View full text Add to dashboard Cite

We report here the isolation, characterization and nucleotide sequence of clones encoding the maize chloroplastic NADP-malate dehydrogenase (NADP-MDH) which functions in the C4 cycle of photosynthesis. A nearly full-length NADP-MDH cDNA clone was isolated using antibodies against the purified protein. This clone hybridizes to a 1600 base mRNA that is eight times more abundant in light-grown maize leaves than in etiolated leaves. Transcription in leaves begins 230 bp upstream of the predicted start of translation, as shown by primer extension experiments. The encoded amino acid sequence predicts that NADP-MDH is synthesized as a preprotein of 432 amino acids (46 865 Da) which is processed into a mature protein of 375 amino acids (40 934 Da) with removal of a 57 amino acid transit peptide (5 931 Da). We identify regions of similarity between the maize NADP-MDH and other MDH polypeptides.

show abstract

“…The enzyme activity was determined according to Zimmermann et al [Ill. Under the present experimental conditions, oxidized fructose bisphosphatase has no activity. Measurement of malate dehydrogenase activation was effected by incubating the enzyme with thioredoxin and dithiothreitol for different time periods before testing the enzyme activity according to Fertk et al [17].…”

Section: Methodsmentioning

confidence: 99%

Affinity chromatography, on fructose‐bisphosphatase–Sepharose, of two chloroplastic thioredoxins F

Buc

Rivière

Gontero

et al. 1984

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

A new method of purification of chloroplastic thioredoxins has been presented. This method is based on affinity chromatography on fructose‐bisphosphatase—Sepharose columns. Two thioredoxin, fA and fB, may be extracted and purified to homogeneity from the same leaf extract. Whereas fA is monomeric and has an Mr of 11400 ± 500, fB is dimeric with an Mr of 18000 ± 600. The dimer dissociates in two halves in the ultracentrifuge under the effect of high pressures. Raising the ionic strength results in the same effect. Thioredoxins fA and fB activate to similar extents chloroplastic fructose bisphosphatase and NADP–malate dehydrogenase. Chloroplastic sedoheptulose bisphosphatase is activated by thioredoxin fB but not by thioredoxin fA.

show abstract

Molecular properties and thioredoxin‐mediated activation of spinach chloroplastic NADP‐malate dehydrogenase

Cited by 20 publications

References 11 publications

Pyruvate,Pi Dikinase and NADP-Malate Dehydrogenase in C4 Photosynthesis: Properties and Mechanism of Light/Dark Regulation

Pyruvate,Pi Dikinase and NADP-Malate Dehydrogenase in C4 Photosynthesis: Properties and Mechanism of Light/Dark Regulation

Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization

Affinity chromatography, on fructose‐bisphosphatase–Sepharose, of two chloroplastic thioredoxins F

Contact Info

Product

Resources

About