Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics

Yasui, Takahiro; Paisrisarn, Piyawan; Yanagida, Takeshi; Konakade, Yuki; Nakamura, Yuta; Nagashima, Kazuki; Musa, Marina; Thiodorus, Ivan Adiyasa; Takahashi, H.; Naganawa, Tsuyoshi; Shimada, Taisuke; Kaji, Noritada; Ochiya, Takahiro; Kawai, Tomoji; Baba, Yoshinobu

doi:10.1016/j.bios.2021.113589

Cited by 21 publications

(31 citation statements)

References 37 publications

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“…Although the difference in atomic arrangement on a nanoscale is subtle, it is expected to cause a large difference in the physical and chemical properties of the nanomaterials, such as their EV capture efficiency. 55,56 We anticipated that the preferential capture of the negatively charged EVs 14,43,57 onto the positively charged surfaces with different crystal planes of ZnO nanowires would lead to the higher EV capture efficiency.…”

Section: Resultsmentioning

confidence: 99%

“…6–9 This high surface-to-volume ratio makes the nanowires very sensitive to changes in surface chemistry and permits rapid interaction with comparatively small sizes of biomolecules including extracellular vesicles (EVs) and nucleic acids giving rise to their prevalence in isolation and detection technology development. 10–15…”

Section: Introductionmentioning

confidence: 99%

“…42,43 We have initiated studies exploiting ZnO nanowire integrated microfluidic devices for label-free capture of EVs through surface charge-based capture which can collect massive numbers of EVs and their molecular cargos. 10,14 However, comprehensive studies of nanowires with a diverse crystal structure and morphology system have not been made yet.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9] This high surface-to-volume ratio makes the nanowires very sensitive to changes in surface chemistry and permits rapid interaction with comparatively small sizes of biomolecules including extracellular vesicles (EVs) and nucleic acids giving rise to their prevalence in isolation and detection technology development. [10][11][12][13][14][15] The physical phenomena in the radial and axial directions and the high surface-to-volume ratios of the nanowires have been controlled and that has led to an explosion of applications utilizing these structures. Getting preferential nucleation at the ZnO (0001) polar plane and suppressing the lateral growth, such as of the (1010) plane, are key essentials to produce a well-defined nanowire structure.…”

Section: Introductionmentioning

confidence: 99%

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Tailoring ZnO nanowire crystallinity and morphology for label-free capturing of extracellular vesicles

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tailoring ZnO nanowire crystallinity and morphology for label-free capturing of extracellular vesicles

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“…Extracellular vesicles (EVs) present promising characteristics as biomarkers for the diagnosis of early cancers [ 9 ]. EVs, defined as a heterogeneous group of membrane structures originated from cells, include apoptotic bodies (500–2000 nm), microvesicles (100–1000 nm), and exosomes (30–150 nm), which exist in biological fluids and participate in a variety of biological processes [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

2022

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Objective Ovarian cancer (OC) is a major threat to women’s health. Mesenchymal stem cells (MSCs) are key regulators in cellular communication by secreting extracellular vesicles (EVs) that are involved in OC. This study probed into the mechanism of human MSCs derived-EVs (hMSC-EVs) in regulating OC cell growth and chemotherapy resistance. Methods hMSCs and EVs were isolated and identified. After adding EVs, the uptake of EVs by OC CAOV3/ES2 cells (for in vitro studies), and cell proliferation, migration, and invasion were detected. Downregulated miRNAs in hMSC-EVs were screened and miR-18a-5p expression in OC patients was detected. The prognosis of OC patients was analyzed. Binding sites of miR-18a-5p and NACC1 were predicted and validated. NACC1 expression in OC tissues was measured by RT-qPCR, and its correlation with miR-18a-5p was analyzed by Pearson method. AKT/mTOR pathway activation was assessed by WB. The cisplatin sensitivity of EVs-treated CAOV3 cells was evaluated via MTT assay and tested by tumor formation assay in nude mice. Results hMSC-EVs suppressed OC cell proliferation, migration, and invasion. miR-18a-5p was downregulated in OC and miR-18a-5p low expression was associated with a poor prognosis. EV-encapsulated miR-18a-5p targeted NACC1. NACC1 was upregulated in OC tissues. miR-18a-5p knockdown and NACC1 overexpression both annulled the inhibition of hMSC-EVs on OC cell growth. AKT and mTOR were elevated in OC and NACC1 activated the AKT/mTOR pathway in OC cells. hMSC-EVs promoted cisplatin sensitivity of OC cells by carrying miR-18a-5p. Conclusion hMSC-EVs-derived miR-18a-5p inhibits OC cell proliferation, migration, invasion, and chemotherapy resistance.

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Nanostructures and Nanotechnologies for the Detection of Extracellular Vesicle

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et al. 2022

Advanced Biology

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characterization, [1] liquid biopsy analyzes the biomarkers in various body fluids, predominantly the blood, enables minimally invasive, dynamic, and integral molecular analysis of tumors and the detection of minimal residual diseases, provides important information for the early diagnosis, prognostication, and the monitoring of tumor progression and treatment response, and would ultimately facilitate precision medicine and personalized cancer management. Extracellular vesicles (EVs) are emerging liquid biopsies that can provide comprehensive molecular information at the level of DNA, RNA, and protein, allowing for the multicomponent analysis of cancers. The detection and molecular analysis of EVs are challenging due to their small size, and the difficulties to purify them from the multicomponent serum or plasma samples. Nanomaterials, nanostructures, and nanotechnologies have shown their significant advantages in the high-purity isolation and the high-sensitive and high-specific detection of EVs. Nanotechnology-based liquid biopsy holds great potential as companion or surrogate of tissue biopsy in the clinical practice.Currently, some businesses have built a product pipeline and service platforms around their unique ability of using EVs to identify biomarkers for disease surveillance and diagnosis. ExoDx Prostate IntelliScore (EPI) from Exosome Diagnostics (ExosomeDX) [2] has been approved by the US Food and Drug Administration (FDA) for the diagnosis of prostate cancer, and ExoDX Lung (ALK), a biopsy product for the analysis of EVsderived RNA from blood samples, has been approved as a clinical assessment tool for gene mutation-associated nonsmall cell lung cancer (NSCLC). Recently, by deriving differentiated detection parameters, a Nanoparticle EXOsome Sensing (NEXOS) technology was shown to detect sEVs ultrasensitively and multidimensionally, [3] leading to a pilot study of liquid biopsy in liver cancer supported by Roche Diagnostics. Therefore, developing diagnostic strategies based on EV capture, detection, and analysis is of paramount importance to increase the effectiveness of cancer diagnosis.In this review, we focus on the development of nanomaterials, nanostructures, and nanotechniques for the detection and molecular characterization of EVs. The advantages of nanotechnologies in enhancing the capture efficiency, sensitivity, and specificity, and the challenges and opportunities of the nanotechnology-based liquid biopsy for the routine clinical practice are discussed.Liquid biopsy has been taken as a minimally invasive examination and a promising surrogate to the clinically applied tissue-based test for the diagnosis and molecular analysis of cancer. Extracellular vesicles (EVs) carry complex molecular information from the tumor, allowing for the multicomponent analysis of cancer and would be beneficial to personalized medicine. In this review, the advanced nanomaterials and nanotechniques for the detection and molecular profiling of EVs, highlight the advantages of nanotechnology in the high-purity iso...

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Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics

Cited by 21 publications

References 37 publications

Tailoring ZnO nanowire crystallinity and morphology for label-free capturing of extracellular vesicles

Tailoring ZnO nanowire crystallinity and morphology for label-free capturing of extracellular vesicles

miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

Nanostructures and Nanotechnologies for the Detection of Extracellular Vesicle

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