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The liver contributes to lipid metabolism as the hub of fat synthesis. Long non‐coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes’ lipid metabolism. Thirty‐two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR‐146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)‐146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high‐fat diet modulated the expression of lncRNA 21 686 and miR‐146b (p‐value < 0.001). Moreover, there was a significant increase in 1‐acyl‐sn‐glycerol‐3‐phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat‐related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p‐value < 0.05). Overexpression of miR‐146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR‐146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR‐146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.
The liver contributes to lipid metabolism as the hub of fat synthesis. Long non‐coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes’ lipid metabolism. Thirty‐two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR‐146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)‐146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high‐fat diet modulated the expression of lncRNA 21 686 and miR‐146b (p‐value < 0.001). Moreover, there was a significant increase in 1‐acyl‐sn‐glycerol‐3‐phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat‐related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p‐value < 0.05). Overexpression of miR‐146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR‐146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR‐146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.
The Yangtze finless porpoise (Neophocaena asiaeorientalis, YFP) is the only extant cetacean in the Yangtze River, and is listed as critically endangered species in the IUCN Red List. There are significant differences in growth and development between male and female YFPs. In order to reveal the potential mechanism of non-coding RNA (ncRNA) involved in this phenomenon, this study took female and male YFP blood as experimental samples, built a database for RNA transcriptome sequencing, and detected ncRNA gene expression profiles such as circRNA, miRNA and lncRNA, and screened differentially expressed genes (DEGs), and performed GO and KEGG functional annotation analysis on DEGs. A total of 205 differentially expressed circRNAs were detected, 87 up-regulated and 118 down-regulated in female YFPs, and the enriched items mainly included energy metabolism and nutritional development of nerves. There were 122 differentially expressed lncRNAs, 54 up-regulated and 68 down-regulated in female YFPs, and the enriched items mainly included heme synthesis and metabolism, immune regulation and immune function. There were 48 differentially expressed miRNAs, 32 up-regulated and 16 down-regulated in female YFPs, and the enriched items mainly included cancer occurrence, energy metabolism and signal transduction. Real-time PCR verified the expression levels of MAPK1, IRS1, ALAD and CIQC were consistent with the sequencing results. This study revealed that ncRNA differentially expressed genes may be involved in the mechanism of sex differences in growth through metabolism, immunity and signal transduction, which provides a new perspective for explaining the growth differences between male and female YFPs, and provides a scientific reference for the protection of YFPs.
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