Molecular prevalence of Cryptosporidium isolates among Egyptian children with cancer

Mohammad, Samira; Ali, Magda Saad; Abdelrahman, Sara; Moustafa, Raghda Abdelrahman

doi:10.1007/s12639-020-01345-y

Cited by 6 publications

(2 citation statements)

References 48 publications

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“…The poor sensitivity exhibited by MZN staining is likely due to the presence of a number of acid-fast bacteria in feces and other fecal structures that are similar in size to Cryptosporidium, including yeast, pollen and other protozoa like cyclospora. Another reason is that poor stain absorption by Oocyst occasionally [21][22][23].…”

Section: Discussionmentioning

confidence: 99%

The efficacy of modified Ziehl-Neelsen (MZN) and enzyme linked immunosorbent assay (ELISA) and molecular techniques for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients

Abdullah,

Taha,

Taher

et al. 2024

Preprint

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Background: Cryptosporidiosis diagnosis based on demonstration of the parasite microscopically in the stool through modified Ziehl-Neelsen (MZN) or auramine phenol staining techniques. The primary goal of this study was to compare between the efficacy of cryptospridia antigen detection potential in the fecal specimens using ELISA and Oocyst detection by MZN staining methods and molecular techniques for the diagnosis of human cryptosporidiosis among some Sudanese patients with gastrointestinal cancer. One hundred and eighty-one patients suspected of having gasterointestinal cancer based on their clinical presentation were enrolled in this study. Cryptosporidiosis was diagnosed using polymerase chain reaction (PCR). MZN staining was conducted for fresh stool samples to detect Cryptosporidium Oocyst. Results: Of 181 cancer suspected patients, 55 (30.5%) were confirmed as having gastrointestinal cancer. Of the 55 cancer patients 41(74.5%) were confirmed as having colorectal cancer, 7 (12.7%) small intestine cancer, 4(7.3%) liver cancer, 3(5.5%) esophageal cancer. Of the confirmed cancer patients MZN identified 24 (43.6%) and ELISA identified 31(56.4%) as having cryptospridia Oocyst and antigens in their fecal samples, respectively. Based on PCR outcome, the infection rates was significantly higher in colorectal cancer patients than in other cancer patients (P = 0.02). Depending on the criteria of ‘true positive’ samples, sensitivity of MZN and ELISA was 81.06% and 94.25% respectively. Conclusions: Fecal antigen ELISA detection test has the advantage of accurate diagnosis of Cryptosporidiosis over MZN staining technique especially in immunocompromised patients.

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Section: Discussionmentioning

confidence: 99%

The efficacy of modified Ziehl-Neelsen (MZN) and enzyme linked immunosorbent assay (ELISA) and molecular techniques for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients

Abdullah,

Taha,

Taher

et al. 2024

Preprint

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“…Molecular genotyping tools, such as polymerase chain reaction (PCR; 830 bp), restriction fragment length polymorphism (RFLP; SspI and VspI restrictions fragment), quantitative polymerase chain reaction (qPCR) and nested PCR, have been used to identify different Cryptosporidium species e.g. C. parvum, C. hominis, C. meleagridis at genetic level (Falohun et al 2021;Fan et al 2021;Mohammad et al 2021). The glycoprotein gene (gp60/gp40/15) with a molecular weight of 60 kDa is widely used to subtype Cryptosporidium species such as C. hominis, C. parvum, C. meleagridis and C. ubiquitum (Li et al 2021).…”

Section: Micro and Macro Characteristics Of The Pathogenmentioning

confidence: 99%

Cryptosporidiosis

2021

Int J Vet Sci

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Current status of intestinal parasitosis among patients attending teaching hospitals in Zagazig district, Northeastern Egypt

Omar

Abdelal²

2022

Parasitol Res

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Almost 80% of health problems in the developing world are due to malnutrition and infectious diseases, which are mainly parasitic. Updated records on the prevalence of parasitic infections and the potential risk factors are essential to enhancing control strategies. Therefore, this study was conducted to evaluate the current situation of intestinal parasitism among patients attending teaching hospitals in Zagazig district, Northeastern Egypt. The study involved five hundred cases. They were all subjected to faecal examination using direct smear measure and two commercial faecal concentrators: Mini-Parasep® solvent-free and Mini-FLOTAC procedures. Mini-FLOTAC was performed with two solutions (FS2: saturated sodium chloride and FS7: zinc sulphate). The overall prevalence of intestinal parasitic infections was 56%. Different species were identified, like Giardia lamblia (12.6%), Entamoeba histolytica/dispar (10%), Ascaris lumbricoides (8.8%) and Hymenolepis nana (8.6%). Data analyses revealed a significant association of intestinal parasitism with different socio-demographic features of the participants. Our results showed a better diagnostic performance of Mini-Parasep® in the overall recovery of intestinal parasites. It was more accurate than Mini-FLOTAC in diagnosing both helminths and protozoan infections. Mini-FLOTAC (FS2) exhibited a higher sensitivity than FS7 for helminth recovery (74.6% vs 53.4%), while FS7 was more sensitive for protozoan infections (50.6% vs 43.8%). Intestinal parasitosis remains a challenging health problem in Zagazig city, wherever reliable diagnostic approaches are limited. Thus, our study has proposed the value of the commercial concentrators (Mini-Parasep® and Mini-FLOTAC) as alternative techniques for diagnosing a large variety of parasite species in resource-constrained settings.

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Molecular prevalence of Cryptosporidium isolates among Egyptian children with cancer

Cited by 6 publications

References 48 publications

The efficacy of modified Ziehl-Neelsen (MZN) and enzyme linked immunosorbent assay (ELISA) and molecular techniques for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients

The efficacy of modified Ziehl-Neelsen (MZN) and enzyme linked immunosorbent assay (ELISA) and molecular techniques for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients

Cryptosporidiosis

Current status of intestinal parasitosis among patients attending teaching hospitals in Zagazig district, Northeastern Egypt

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