Molecular pollution biomarkers in Algerian mouse (Mus spretus) to assess the efects of Aznalcollar disaster on Doñana National Park (Spain)

Ruiz-Laguna, Julia; García, Cristina Moya; Peinado, J.; Bonilla, Diego A.; Moreno, Sacramento; Cristaldi, M.; López-Barea, Juán

doi:10.1016/s1095-6433(00)80189-0

Cited by 4 publications

(10 citation statements)

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“…inbred laboratory mouse strains. The laboratory mouse Wild M. spretus mice were captured with live Sheris a good tool for understanding genetic influences man traps as described previously (21). Animals were of differences in phenotypes.…”

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confidence: 99%

Tissue, Species, and Environmental Differences in Absolute Quantities of Murine mRNAs Coding for Alpha, Mu, Omega, Pi, and Theta Glutathione <I>S</I>-Transferases

Ruiz-Laguna

Abril²,

Prieto‐Álamo³

et al. 2005

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This article reports the first absolute quantitative analysis of expression patterns of murine transcripts (Gsta1/2, Gsta3, Gsta4, Gstm1, Gstm2, Gstm3, Gsto1, Gstp1/2, Gstt1, Gstt2) coding for most glutathione S-transferases (GSTs) of alpha, mu, omega, pi, and theta classes. We examine how the steady-state numbers of transcripts are modulated in association with: three animal organs (liver, kidney, and lung) where extensive detoxification occurs; two species (Mus musculus and Mus spretus) representing common laboratory and aboriginal mice; and two genetic and animal living conditions (wild-derived inbred animals and free-living mice). Moreover, quantitations performed examine how the pulmonary steady-state Gst mRNA amounts are affected in M. musculus by paraquat (a superoxide generator), and in M. spretus by dwelling at a polluted area. The results point to complex tissue-, species-, and life condition-dependent expression of the investigated transcripts. Among others, they show: i) the ubiquity of most transcripts, except Gstm3 mRNA that was virtually absent or at very low amounts (< or = 0.001 molecules/pg) in kidney and lung of M. spretus; ii) unique expression profiles for each transcript and mouse organ examined; iii) outstanding species-specific differences in basal amounts of most Gst mRNAs, this effect being most apparent in the case of Gsta1/2, Gsta3, Gstm2, Gsto1, Gstt1, and Gstt2; iv) paraquat-induced upregulation of most Gst mRNAs, with the notable exception of those coding for theta class GSTs; v) a tendency for mice dwelling at a wildlife reserve of having lower and more homogeneous Gsta3 mRNA levels than those collected in an anthropogenic environment.

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confidence: 99%

Tissue, Species, and Environmental Differences in Absolute Quantities of Murine mRNAs Coding for Alpha, Mu, Omega, Pi, and Theta Glutathione <I>S</I>-Transferases

Ruiz-Laguna

Abril²,

Prieto‐Álamo³

et al. 2005

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“…Before the spill mice from the "Brazo de la Torre" (Fig. 2) had GSHPx and EROD activities close to animals of Huelva Industrial Park, suggesting similar levels of oxidants and aromatic contaminants at both sites (Ruiz-Laguna et al, 2001). Six months after the spill, mice from the lower Guadiamar areas, also showed significantly higher GSTc and GSTm activities, altered levels of several antioxidant enzymes, and a highly oxidized GSSG/GSH status.…”

Section: Effects Of Aznalcóllar Spill Assessed In M Spretusmentioning

confidence: 92%

“…This small animal attains high population densities, typically inhabits marshlands, and feeds on seeds, insects and small invertebrates around its burrow. This free-living rodent has proved to be an excellent bioindicator of contamination in terrestrial ecosystems (Nunes et al, 2001;Ruiz-Laguna et al, 2001;Bonilla-Valverde et al, 2004). The two murine species separated more than one million years ago (Gao & Zhang, 2003) and exhibit a relatively high gene sequence homology.…”

Section: Fig 1 Procambarus Clarkii and Mus Spretus And The Model Lmentioning

confidence: 99%

“…To assess their possible biological effects, biochemical biomarkers were assessed in M. spretus from several Doñana areas and along the Guadiamar course (Ruiz-Laguna et al, 2001). Biomarkers assayed responded to different contaminant types: 1) Metals and prooxidant compounds (CAT, SOD, GSSGRase, GSHPx, G6PDH, MDA, GSSG/GSH status).…”

Section: Effects Of Aznalcóllar Spill Assessed In M Spretusmentioning

confidence: 99%

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New Methodologies for Assessing the Presence and Ecological Effects of Pesticides in Doñana National Park (SW Spain)

Pueyo¹,

Gómez-Ariza²,

Bello-López³

et al. 2011

Pesticides in the Modern World - Trends in Pesticides Analysis

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“…Previous studies (using CREST staining analysis) indicated a cytogenetic damage in mice collected near Aznalcollar mine, suggesting that animals had been exposed to aneugenic substances (Festa et al, 2003;Cristaldi et al, in press). Moreover the alteration of some biochemical biomarkers in the liver of these animals (Ruiz-Laguna et al, 2001) and modifications of the protein expression profile in the gills of S. plana clams (RomeroRuiz et al, 2006) were observed.…”

Section: Introductionmentioning

confidence: 97%

Cell‐cycle protein modifications in the kidney of Mus spretus from Doñana National Park

Leoni

Torrice

Chiuchiarelli

et al. 2008

Environmental Toxicology

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On April 1998 a tailing dam of the Aznalcollar pyrite mine partially collapsed and released to the Guadiamar river acidic water and mud containing toxic metals threatening the Doñ ana National Park, a Spanish wildlife reserve located near the estuary of Guadalquivir river. To assess the possible biological effects on terrestrial ecosystems, biochemical markers were assayed in the kidneys of Algerian mice (Mus spretus) collected in several areas of Doñ ana and Guadiamar river. Biomarkers assayed are proteins involved in cell cycle regulation, in particular cyclins and their associated kinases, and some cell cycle inhibitors. Moreover Mitogen Activated Protein Kinases (MAPK), a signal transduction system involved in cell division, p53, a protein involved in growth arrest after DNA damage, and HSP70, an early stressinduced protein, were assayed. The kidneys of animals collected one year after the ecological disaster had increased levels of PCNA (proliferating cell nuclear antigen), indicating an increased number of cells in the S phase of cell cycle. This shift of cells from G0 to S phase is due to increased levels of cyclins D1, E, and A, to decreased levels of p21 and p27 cdk inhibitors, and to activation of MAPK cascade. On the other hand, p53 and HSP70 levels are not changed. These data demonstrate that the presence of toxic metals after ecological disaster provoked the induction of kidney cell proliferation interpretable as a compensatory cell growth after tissue damage and apoptosis, and that could lead to the genomic instability characteristic of cancer cell. # 2008 Wiley Periodicals, Inc. Environ Toxicol 23: 44-51, 2008

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Molecular pollution biomarkers in Algerian mouse (Mus spretus) to assess the efects of Aznalcollar disaster on Doñana National Park (Spain)

Cited by 4 publications

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Tissue, Species, and Environmental Differences in Absolute Quantities of Murine mRNAs Coding for Alpha, Mu, Omega, Pi, and Theta Glutathione <I>S</I>-Transferases

Tissue, Species, and Environmental Differences in Absolute Quantities of Murine mRNAs Coding for Alpha, Mu, Omega, Pi, and Theta Glutathione <I>S</I>-Transferases

New Methodologies for Assessing the Presence and Ecological Effects of Pesticides in Doñana National Park (SW Spain)

Cell‐cycle protein modifications in the kidney of Mus spretus from Doñana National Park

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