Molecular physiology of the SERCA and SPCA pumps

Wuytack, Frank; Raeymaekers, Luc; Missiaen, Ludwig

doi:10.1016/s0143416002001847

Cited by 286 publications

(251 citation statements)

References 209 publications

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“…78,79 The ER membrane contains Ca 2ϩ pumps (the sarco[endo]plasmic reticulum ATP-ases (or SERCAs) that transport Ca 2ϩ into the ER lumen. 80 The intra-ER Ca 2ϩ concentration is very high (range, 0.5 to 1 mM), 81,82 which creates a steep concentration gradient aimed at the cytosol. Importantly, the lumen of the ER is internally continuous and Ca 2ϩ can rapidly equilibrate within the organelle through unopposed diffusion.…”

Section: Signaling In Neuronal-glial Circuits 1) Glial Cells Express mentioning

confidence: 99%

Astrocytes in Alzheimer's Disease

et al. 2010

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Summary:The circuitry of the human brain is formed by neuronal networks embedded into astroglial syncytia. The astrocytes perform numerous functions, providing for the overall brain homeostasis, assisting in neurogenesis, determining the micro-architecture of the grey matter, and defending the brain through evolutionary conserved astrogliosis programs.Astroglial cells are engaged in neurological diseases by determining the progression and outcome of neuropathological process. Astrocytes are specifically involved in various neurodegenerative diseases, including Alzheimer's disease, amyotrophic lateral sclerosis, Parkinson's disease, and various forms of dementia. Recent evidence suggest that early stages of neurodegenerative processes are associated with atrophy of astroglia, which causes disruptions in synaptic connectivity, disbalance in neurotransmitter homeostasis, and neuronal death through increased excitotoxicity. At the later stages, astrocytes become activated and contribute to the neuroinflammatory component of neurodegeneration.

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Section: Signaling In Neuronal-glial Circuits 1) Glial Cells Express mentioning

confidence: 99%

Astrocytes in Alzheimer's Disease

et al. 2010

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“…Overexpression of wild-type or ER-restricted BCL-2/BCL-xL, on the other hand, provides resistance to Ca 2 þ -dependent apoptotic stimuli, due to decreased release of ER Ca 2 þ and a resultant decrease in mitochondrial Ca 2 þ uptake (Annis et al, 2001;Pinton et al, 2001;Rudner et al, 2001). Ca 2 þ release from the ER occurs primarily through the inositol 1,4,5-triphosphate receptor (IP 3 R) and the ryanodine receptor (Zalk et al, 2007) protein families, whereas Ca 2 þ reuptake is dependent on the sarco/ER Ca 2 þ -ATPase (SERCA) (Wuytack et al, 2002). BCL-2/BCL-xL may regulate ER Ca 2 þ signaling through interaction with the IP 3 R (Chen et al, 2004b;White et al, 2005;Hanson et al, 2008), and/or alteration of the IP 3 R phosphorylation state (Oakes et al, 2005;Xu et al, 2007).…”

Section: Apoptosis and Er Ca 2 þ Releasementioning

confidence: 99%

The endoplasmic reticulum in apoptosis and autophagy: role of the BCL-2 protein family

2008

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“…Molecular cloning has identified three separate SERCA genes termed SERCA1 (ATP2A1), SERCA2 (ATP2A2), and SERCA3 (ATP2A3). The protein products of ATP2A1-3 (SERCA1-3) are characterized by tissue-specific expression and differences in catalytic properties (Wu et al, 1985;Wuytack et al, 2002;Strehler and Treiman, 2004).…”

Section: Introductionmentioning

confidence: 99%

Serca isoform expression in the mammalian retina

Križaj¹

2005

Experimental Eye Research

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The sarcoplasmic-endoplasmic reticulum calcium ATPase (SERCA) is a key intracellular calcium transporter, which regulates cellular calcium concentration [Ca 2+ ] by transporting Ca 2+ ions from the cytosol into the endoplasmic reticulum. SERCA-mediated Ca 2+ sequestration controls proper folding of newly synthesized proteins within the ER as well as the timing and spatial patterning of depolarization-evoked Ca 2+ responses in the cytoplasm. We studied expression and distribution of all three SERCA isoforms in the mouse retina using isoform-specific antibodies. No immunostaining was observed with the SERCA1 antibody. SERCA2 was expressed in photoreceptor inner segments, amacrine and ganglion cells of the mouse retina. Similar SERCA2 localization was observed in adult rat, macaque and ground squirrel retinas. Analysis of distribution of SERCA2 immunofluorescence in the developing mouse retina revealed prominent SERCA2 signals throughout postnatal development. The antibody raised against the SERCA3 isoform labeled inner segments of photoreceptors and cell bodies in the inner nuclear layer of the mouse retina. The SERCA3 signal was detected in the inner plexiform layer of the early postnatal retina, but moved by P10 to the outer retina where it was concentrated in outer segments of cones. These results indicate that SERCA2 represents the dominant SERCA isoform in the mammalian retina. SERCA3 may contribute to calcium regulation in photoreceptors and bipolar cells.

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Molecular physiology of the SERCA and SPCA pumps

Cited by 286 publications

References 209 publications

Astrocytes in Alzheimer's Disease

Astrocytes in Alzheimer's Disease

The endoplasmic reticulum in apoptosis and autophagy: role of the BCL-2 protein family

Serca isoform expression in the mammalian retina

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