Molecular physiology of P2 receptors in the central nervous system

Illéš, Peter; Ribeiro, Joaquim A.

doi:10.1016/j.ejphar.2003.10.030

Cited by 141 publications

(91 citation statements)

References 188 publications

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“…In addition, ATP is released as a neurotransmitter in the peripheral and central nervous system [2][3][4]. ATP acts on two classes of receptors, referred to as P2X and P2Y receptors.…”

Section: Introductionmentioning

confidence: 99%

P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb

Fischer

Rotermund

Lohr

et al. 2011

Purinergic Signalling

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It has recently been shown that adenosine-5′-triphosphate (ATP) is released together with glutamate from sensory axons in the olfactory bulb, where it stimulates calcium signaling in glial cells, while responses in identified neurons to ATP have not been recorded in the olfactory bulb yet. We used photolysis of caged ATP to elicit a rapid rise in ATP and measured whole-cell current responses in mitral cells, the output neurons of the olfactory bulb, in acute mouse brain slices. Wide-field photolysis of caged ATP evoked an increase in synaptic inputs in mitral cells, indicating an ATP-dependent increase in network activity. The increase in synaptic activity was accompanied by calcium transients in the dendritic tuft of the mitral cell, as measured by confocal calcium imaging. The stimulating effect of ATP on the network activity could be mimicked by photo release of caged adenosine 5′-diphosphate, and was inhibited by the P2Y 1 receptor antagonist MRS 2179. Local photolysis of caged ATP in the glomerulus innervated by the dendritic tuft of the recorded mitral cell elicited currents similar to those evoked by wide-field illumination. The results indicate that activation of P2Y 1 receptors in the glomerulus can stimulate network activity in the olfactory bulb.

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“…In addition, ATP is released as a neurotransmitter in the peripheral and central nervous system [2][3][4]. ATP acts on two classes of receptors, referred to as P2X and P2Y receptors.…”

Section: Introductionmentioning

confidence: 99%

P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb

Fischer

Rotermund

Lohr

et al. 2011

Purinergic Signalling

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“…This nucleotide may be released from nerve terminals/glial cells by exocytotic mechanisms [37], or only from astrocytic cells via connexin hemichannels, volumeregulated anion channels, ATP-binding cassette transporters and the cystic fibrosis transmembrane regulator [38]. Functional P2X7 receptors have been identified both at cultured astrocytes [39][40][41][42] and astrocytes situated in brain slices [43].…”

Section: Discussionmentioning

confidence: 99%

Potentiation of the glutamatergic synaptic input to rat locus coeruleus neurons by P2X7 receptors

Khakpay

Polster

Köles

et al. 2010

Purinergic Signalling

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Locus coeruleus (LC) neurons in a rat brain slice preparation were superfused with a Mg 2+ -free and bicuculline-containing external medium. Under these conditions, glutamatergic spontaneous excitatory postsynaptic currents (sEPSCs) were recorded by means of the whole-cell patch-clamp method. ATP, as well as its structural analogue 2-methylthio ATP (2-MeSATP), both caused transient inward currents, which were outlasted by an increase in the frequency but not the amplitude of the sEPSCs. PPADS, but not suramin or reactive blue 2 counteracted both effects of 2-MeSATP. By contrast, α,β-methylene ATP (α,β-meATP), UTP and BzATP did not cause an inward current response. Of these latter agonists, only BzATP slightly facilitated the sEPSC amplitude and strongly potentiated its frequency. PPADS and Brilliant Blue G, as well as fluorocitric acid and aminoadipic acid prevented the activity of BzATP. Furthermore, BzATP caused a similar facilitation of the miniature (m)EPSC (recorded in the presence of tetrodotoxin) and sEPSC frequencies (recorded in its absence). Eventually, capsaicin augmented the frequency of the sEPSCs in a capsazepine-, but not PPADS-antagonizable, manner. In conclusion, the stimulation of astrocytic P2X7 receptors appears to lead to the outflow of a signalling molecule, which presynaptically increases the spontaneous release of glutamate onto LC neurons from their afferent fibre tracts. It is suggested, that the two algogenic compounds ATP and capsaicin utilise separate receptor systems to potentiate the release of glutamate and in consequence to increase the excitability of LC neurons.

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“…The role of P1 and P2 receptors in the function of immune cells (e.g., neutrophils, eosinophils, monocytes, macrophages, mast cells, and lymphocytes) has been well described [26,[52][53][54][55][56][57], and the studies suggest that these receptors regulate cellular responses associated with inflammatory diseases. P2Rs are expressed at presynaptic nerve terminals where P2X1, P2X2, and P2X3 receptors have facilitatory, whereas P2Y 1 , P2Y 2 , and P2Y 4 receptors have inhibitory roles in synaptic transmission [10,[58][59][60][61][62]. Studies also have shown that postsynaptic P2 receptors including P2X3, P2Y 4 , and P2Y 1 receptors are involved in neuromodulation [10,[63][64][65], where they regulate either transmitter release or postsynaptic sensitivity to other neurotransmitters.…”

Section: P2 Receptors In the Cnsmentioning

confidence: 99%

“…P2Rs are expressed at presynaptic nerve terminals where P2X1, P2X2, and P2X3 receptors have facilitatory, whereas P2Y 1 , P2Y 2 , and P2Y 4 receptors have inhibitory roles in synaptic transmission [10,[58][59][60][61][62]. Studies also have shown that postsynaptic P2 receptors including P2X3, P2Y 4 , and P2Y 1 receptors are involved in neuromodulation [10,[63][64][65], where they regulate either transmitter release or postsynaptic sensitivity to other neurotransmitters.…”

Section: P2 Receptors In the Cnsmentioning

confidence: 99%

“…This functional complexity is well manifested in the central nervous system (CNS) where 7 P2X and 8 P2Y receptor subtypes are expressed under a range of conditions in several different interacting cell types [6,[8][9][10][11][12]. Accordingly, studies on P2 receptor functions in the brain must consider the combined contributions of P2X and P2Y receptors expressed in neurons, microglial cells, astrocytes, and endothelium [10,[13][14][15]. In addition, the major ligand that activates many of these P2 receptor subtypes is ATP, released in the course of neurotransmission or under proinflammatory or cell apoptotic conditions [1,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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