Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance

Abstract

Water deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol

Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

“…The ability of AdGolS3 to improve the tolerance to three different types of abiotic stresses corroborates previous studies that signal GolS as a regulator of the synthesis of galactinol and raffinose, and indirectly other sugars, in stress-tolerant transgenic plants 41,46,[48][49][50][51][52][53] . This may be related to the multiple putative functions of these oligosaccharides in plants.…”

“…In the present study, the overexpression of the AdGolS3 gene increased the abundance of galactinol and raffinose in transgenic Arabidopsis plants and conferred tolerance to drought, salt and osmotic stresses. These results therefore corroborate previous reports demonstrating that the overexpression of GolS genes can successfully impart tolerance to multiple abiotic stresses in the models Arabidopsis and tobacco 5 and also in crop species such as rice, soybean, tomato, and poplar 45,46,49,52 . It is worth noting, however, that the natural accumulation of RFOs in mature seeds to protect the embryo from desiccation is considered an antinutritional factor for many grain legumes 13,17 .…”

“…AdGolS3 showed opposite expression behavior between drought-tolerant and drought-sensitive wild Arachis. The overexpression of GolS genes leads to enhanced tolerance to abiotic stresses (drought, salt, heavy metal, cold and heat), by increasing galactinol and RFO contents in transgenic dicots and monocot species 41,[45][46][47][48][49][50][51][52] . These transgenes were isolated from a number of plant species being the AtGolS2 gene from Arabidopsis, the most commonly used.…”

Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy

“…This provides evidence that one of MdGolS2’s functions during bud dormancy is to synthesize galactinol, leading to increased amounts of RFOs, which will act together in bud protection against limited water supply. Transgenic expression of GolS genes from several different species was also capable of conferring tolerance to water deficit, freezing, heavy metal, or salt stress ( Taji et al , 2002 ; Nishizawa et al , 2008 ; Wang et al , 2009 ; Song et al , 2013 ; Sun et al , 2013 ; Honna et al , 2016 ; Wang 2016 b ; Yu et al , 2017 ; Selvaraj et al , 2017 ). This increased stress tolerance may be due to the ability of galactinol and RFOs to scavenge hydroxyl radicals as well as their osmolyte properties, maintaining cell turgor, and stabilizing cell proteins and membranes ( Nishizawa et al , 2008 ; Elsayed et al , 2014 ; Salvi et al , 2016 ).…”

Seleção de sementes de soja geneticamente modificadas com o gene marcador bar por meio do teste de germinação em solução de glufosinato de amônio

“…Todas as linhagens GMs foram desenvolvidas no Laboratório de Biotecnologia Vegetal da Embrapa Soja, transformadas com diferentes construções gênicas para tolerância à seca, utilizando o gene bar como gene marcador para identificação das plantas transformadas. A caracterização detalhada do desenvolvimento dos genótipos e a confirmação da transformação via PCR foram descritas em estudos prévios realizados pelo nosso grupo de pesquisa (Barbosa et al, 2013;Leite et al, 2014;Marinho et al, 2016;Honna et al, 2016). As linhagens 2Ha11 e 5Ha10 foram transformadas com um gene que codifica para uma enzima-chave na via biossintética do ácido abscísico (ABA); as linhagens 2Ia1 e 2Ia4 foram transformadas com um gene que codifica para um açúcar; as linhagens 2Oa4 e 3Pa4 foram transformadas com um gene que codifica para um fator de transcrição ABA-independente; e as linhagens 1Ea2939 e 1Ea15 foram transformadas com um gene que codifica para um fator de transcrição da via de resposta à seca ABA dependente.…”