Molecular Phylogeny of Tubificid Oligochaetes with Special Emphasis on Tubifex tubifex (Tubificidae)

Beauchamp, Katherine A.; Kathman, R. Deedee; McDowell, T. S.; Hedrick, Ronald P.

doi:10.1006/mpev.2001.0923

Cited by 104 publications

(98 citation statements)

References 42 publications

(49 reference statements)

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“…Tubifex tubifex represents a species complex (Beauchamp et al, 2001;Crottini et al, 2008), showing a variability of the morphological characters (chaetae and other anatomical characters) (Holmquist, 1983). This species complex has a wide ecological valence, tolerating trophic conditions up to eutrophy, and, in environments with high organic matter content, it may become dominant due to its ability to tolerate low levels of oxygen.…”

Section: Discussionmentioning

confidence: 99%

“…Sometimes these characteristics are insufficient and it is necessary to examine mature specimens, because only the morphology of the male reproductive system is a valid character (Brinkhurst, 1971). Molecular analyses have shown that, in some cases, sexual characteristics are insufficient for a specific discrimination (Beauchamp et al, 2001;Envall et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The tubificids (Annelida, Oligochaeta) of Lake Trasimeno and Lake Piediluco in Central Italy, with a study of SEM morphology of some species

Goretti¹,

Marcucci²,

Veroli³

et al. 2014

Turk J Zool

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The tubificids (Annelida, Oligochaeta) of Lake Trasimeno and Lake Piediluco in Central Italy, with a study of SEM morphology of some species

Goretti¹,

Marcucci²,

Veroli³

et al. 2014

Turk J Zool

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“…Preliminary molecular studies, based on analyses of small mitochondrial 16S rDNA data sets, suggested that the morpho-taxon L. hoffmeisteri is likely to encompass cryptic lineages (Beauchamp et al 2001;Erséus and Gustafsson 2009;Marton and Eszterbauer 2012). It is also known that several other, common and widely distributed clitellate morphospecies are complexes of cryptic species (i.e., morphologically more or less indistinguishable, but genetically distinct species) (Erséus and Gustafsson 2009;James et al 2010;Erséus 2014, 2017).…”

Section: Introductionmentioning

confidence: 99%

Extensive cryptic diversity in the cosmopolitan sludge worm Limnodrilus hoffmeisteri (Clitellata, Naididae)

Liu

Fend²,

Martinsson

et al. 2017

Org Divers Evol

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Limnodrilus hoffmeisteri Claparède, 1862 is a common freshwater worm, often regarded as an indicator of organic pollution. The taxonomic status of this species is controversial due to great variation in morphological features. Numerous morphological forms of L. hoffmeisteri are recorded in the literature, especially from Europe and North America. Today, DNA-based species delimitation assumes that species boundaries can be more objectively and effectively estimated using genetic data rather than with morphological data alone. To investigate if L. hoffmeisteri is a single species, 295 worms identified as either L. hoffmeisteri or other similar (congeneric) morphospecies, using currently accepted morphological criteria, were collected from 82 locations in the northern hemisphere. The number of primary species hypotheses (PSHs) was first explored with cytochrome oxidase subunit I (COI), the proposed DNA barcode for animal species, and with data for all specimens. Both automatic barcoding gap discovery (ABGD) and the Bayesian general mixed Yule coalescent (bGMYC) model revealed the existence of ≥25 distinct PSHs (COI lineages) in our dataset. Then, smaller samples of individuals representing major COI lineages were used for exploration of a nuclear locus, the internal transcribed spacer (ITS) region. In the ITS gene tree (81 sequences), generated by BEAST, 16 well-supported terminal groups were found, but not all of these groups were congruent with the PSHs found in the COI tree. As results across these different analyses were inconsistent, we resorted to analyzing reciprocal monophyly between gene trees and used a minimum consensus of all evidence, suggesting that there are 13 separately evolving lineages (=13 species) within our sample. The smallest uncorrected COI p-distance between these species is 12.1%, and the largest intraspecific p-distance is 16.4%, illustrating the problem of species delimitation with a DNAbarcoding gap as the sole criterion. Ten of these species are morphologically identified as BL. hoffmeisteri,^the remaining three can be attributed to morphologically distinct congeneric species. An individual from the type locality in Switzerland was designated as a neotype of L. hoffmeisteri sensu stricto. This worm belongs to one of the ten species, and this lineage is widely distributed in Europe, Asia, and North America. The remaining nine species show a mixed distribution pattern; some appear to be endemic to a restricted area, others are Holarctic. Our results provide clues to the future revalidation of some of the nominal species today placed in synonymy with L. hoffmeisteri. A BEAST analysis, based on previously published and newly generated 16S data, suggested that this complex contains also other species than those studied by us. By integrating additional genetic data, it will be possible to identify these and additional specimens in future studies of Limnodrilus, and the neotype provides a baseline for further revisions of the taxonomy of the L. hoffmeisteri complex.

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“…Application of polymerase chain reaction (PCR) technology (Andree et al 1998, Baldwin & Myklebust 2002, Cavender et al 2004) molecular biology (Sturmbauer et al 1999, Beauchamp et al 2001) and other state-of-the-art analytical techniques led to rapid advances and a deeper understanding of the etiology of M. cerebralis in the aquatic oligochaete host T. tubifex (Gilbert & Granath 2001, Beauchamp et al 2002, 2006, Nehring et al 2013. Beauchamp et al (2001) developed mitochondrial 16S ribosomal DNA markers to parse the T. tubifex species-complex into 6 distinct lineages. Subsequent studies demonstrated that the Lineage III T. tubifex worms are highly receptive to M. cerebralis infection and prodigious producers of the fish-infective triactinomyxon (TAM) actinospores even at a low dose rate of 50 M. cerebralis myxospores worm −1 (Nehring et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Accelerated deactivation of Myxobolus cerebralis myxospores by susceptible and non-susceptible Tubifex tubifex

Nehring¹,

Schisler²,

Chiaramonte³

et al. 2016

Dis. Aquat. Org.

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In the 1990s, the Tubifex tubifex aquatic oligochaete species complex was parsed into 6 separate lineages differing in susceptibility to Myxobolus cerebralis, the myxozoan parasite that can cause whirling disease (WD). Lineage III T. tubifex oligochaetes are highly susceptible to M. cerebralis infection. Lineage I, IV, V and VI oligochaetes are highly resistant or refractory to infection and may function as biological filters by deactivating M. cerebralis myxospores. We designed a 2-phased laboratory experiment using triactinomyxon (TAM) production as the response variable to test that hypothesis. A separate study conducted concurrently demonstrated that M. cerebralis myxospores held in sand and water at temperatures ≤15°C degrade rapidly, becoming almost completely non-viable after 180 d. Those results provided the baseline to assess deactivation of M. cerebralis myxospores by replicates of mixed lineage (I, III, V and VI) and refractory lineage (V and VI) oligochaetes. TAM production was zero among 7 of 8 Lineage V and Lineage VI T. tubifex oligochaete groups exposed to 12 500 M. cerebralis myxospores for 15, 45, 90 and 135 d. Among 4 mixed lineage exposure groups, TAM production averaged 14 641 compared with 2 202 495 among 12 groups of Lineage III oligochaetes. Among the 6 unexposed Lineage III experimental groups seeded into original Phase 1 substrates for the 45, 90 and 135 d treatments during the Phase 2 portion of the study, TAM production was reduced by 98.9, 99.9 and 99.9%, respectively, compared with the average for the 15 d exposure groups. These results are congruent with the hypothesis that Lineage V and Lineage VI T. tubifex oligochaetes can deactivate and destroy M. cerebralis myxospores.

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Molecular Phylogeny of Tubificid Oligochaetes with Special Emphasis on Tubifex tubifex (Tubificidae)

Cited by 104 publications

References 42 publications

The tubificids (Annelida, Oligochaeta) of Lake Trasimeno and Lake Piediluco in Central Italy, with a study of SEM morphology of some species

The tubificids (Annelida, Oligochaeta) of Lake Trasimeno and Lake Piediluco in Central Italy, with a study of SEM morphology of some species

Extensive cryptic diversity in the cosmopolitan sludge worm Limnodrilus hoffmeisteri (Clitellata, Naididae)

Accelerated deactivation of Myxobolus cerebralis myxospores by susceptible and non-susceptible Tubifex tubifex

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