Molecular phylogeny of the Entomophthoromycota

Gryganskyi, Andrii P.; Humber, Richard A.; Smith, Matthew E.; Miadlikovska, Jolanta; Wu, Steven; Voigt, Kerstin; Walther, Grit; Anishchenko, Iryna M.; Vilgalys, Rytas

doi:10.1016/j.ympev.2012.07.026

Cited by 88 publications

(93 citation statements)

References 55 publications

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“…quinquefasciatus sample. An OTU most likely classified as Pandora/Furia , which comprises the majority of the eukaryotic reads in this sample, is a known and exclusive insect pathogen (Gryganskyi et al., 2012). Likewise, Novispirillum, which comprises over 96% of the total bacterial reads in this study, has been isolated from unhealthy‐looking Cx.…”

Section: Discussionmentioning

confidence: 99%

Mosquito vector‐associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod‐borne diseases

Thongsripong

Chandler

Green

et al. 2017

Ecology and Evolution

118

112

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Vector‐borne diseases are a major health burden, yet factors affecting their spread are only partially understood. For example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. Nonetheless, current knowledge of mosquito‐associated microbial communities is limited. To characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas, we employed next‐generation 454 pyrosequencing of 16S and 18S rRNA amplicon libraries, also known as metabarcoding. We investigated pooled whole adult mosquitoes of three medically important vectors, Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus, collected from different habitats across central Thailand where we previously characterized mosquito diversity. Our results indicate that diversity within the mosquito microbiota is low, with the majority of microbes assigned to one or a few taxa. Two of the most common eukaryotic and bacterial genera recovered (Ascogregarina and Wolbachia, respectively) are known mosquito endosymbionts with potentially parasitic and long evolutionary relationships with their hosts. Patterns of microbial composition and diversity appeared to differ by both vector species and habitat for a given species, although high variability between samples suggests a strong stochastic element to microbiota assembly. In general, our findings suggest that multiple factors, such as habitat condition and mosquito species identity, may influence overall microbial community composition, and thus provide a basis for further investigations into the interactions between vectors, their microbial communities, and human‐impacted landscapes that may ultimately affect vector‐borne disease risk.

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Section: Discussionmentioning

confidence: 99%

Mosquito vector‐associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod‐borne diseases

Thongsripong

Chandler

Green

et al. 2017

Ecology and Evolution

118

112

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“…GIB is a very rare emergent fungal infection caused by Basidiobolus spp. Although several species of Basidiobolus have been described in the literature (Gryganskyi et al, 2012), Basidiobolus ranarum was the only one reported in human gastrointestinal cases (Lyon et al, 2001). Nevertheless, in the few published cases, the diagnosis was based on histopathology examination and rarely on tissue culture or serodiagnosis.…”

Section: Discussionmentioning

confidence: 99%

Contribution of Ultra Deep Sequencing in the Clinical Diagnosis of a New Fungal Pathogen Species: Basidiobolus meristosporus

Sitterlé

Rodriguez

Mounier³

et al. 2017

Front. Microbiol.

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Some cases of fungal infection remained undiagnosed, especially when the pathogens are uncommon, require specific conditions for in vitro growth, or when several microbial species are present in the specimen. Ultra-Deep Sequencing (UDS) could be considered as a precise tool in the identification of involved pathogens in order to upgrade patient treatment. In this study, we report the implementation of UDS technology in medical laboratory during the follow-up of an atypical fungal infection case. Thanks to UDS technology, we document the first case of gastro-intestinal basidiobolomycosis (GIB) due to Basidiobolus meristosporus. The diagnosis was suspected after histopathological examination but conventional microbiological methods failed to supply proof. The final diagnosis was made by means of an original approach based on UDS. DNA was extracted from the embedded colon biopsy obtained after hemicolectomy, and a fragment encompassing the internal transcribed spacer (ITS) rDNA region was PCR-amplified. An Amplicon library was then prepared using Genome Sequencer Junior Titanium Kits (Roche/454 Life Sciences) and the library was pyrosequenced on a GS Junior (Roche/454 Life Sciences). Using this method, 2,247 sequences with more than 100 bases were generated and used for UDS analysis. B. meristosporus represented 80% of the sequences, with an average homology of 98.8%. A phylogenetic tree with Basidiobolus reference sequences confirmed the presence of B. meristosporus (bootstrap value of 99%).Conclusion : UDS-based diagnostic approaches are ready to integrate conventional diagnostic testing to improve documentation of infectious disease and the therapeutic management of patients.

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“…However, recent studies based on housekeeping and ribosomal genes have shown that the specialized entomopathogenic entomophthoralean fungi should be recognized as a separate evolutionary unit (Gryganskyi et al, 2012). This provided a basis for erecting the new phylum Entomophthoromycota (Humber, 2012).…”

Section: P Formicae: Relationships With Other Pathogenic Fungimentioning

confidence: 99%

Transcriptome of an entomophthoralean fungus (Pandora formicae) shows molecular machinery adjusted for successful host exploitation and transmission

Małagocka

Grell

Lange

et al. 2015

Journal of Invertebrate Pathology

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Molecular phylogeny of the Entomophthoromycota

Cited by 88 publications

References 55 publications

Mosquito vector‐associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod‐borne diseases

Mosquito vector‐associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod‐borne diseases

Contribution of Ultra Deep Sequencing in the Clinical Diagnosis of a New Fungal Pathogen Species: Basidiobolus meristosporus

Transcriptome of an entomophthoralean fungus (Pandora formicae) shows molecular machinery adjusted for successful host exploitation and transmission

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