Molecular phylogeny and phylogeography of Holarctic species of Pluteus section Pluteus (Agaricales: Pluteaceae), with description of twelve new species

Abstract

The taxonomy and phylogeography of Pluteus section Pluteus in the Holarctic region was investigated using morphological and molecular data. Over 300 specimens spanning the major areas of boreal and temperate forests of the Northern Hemisphere were studied and nrITS and tef1 were obtained for phylogenetic analyses. In order to stabilize the taxonomy of the group all available type collections were studied and, if possible, sequenced. A total of 26 species occurring in Eurasia and North America were recovered in the phylogenetic analyses. Twelve species are described as new (Pluteus rangifer, P. elaphinus, P. hibbettii, P. eos, P. orestes, P. methvenii, P. shikae, P. kovalenkoi, P. leucoborealis, P. sepiicolor, P. oreibatus, P. atrofibrillosus), one is provisionally named (P. parilis) and one variety is raised to species rank (P. americanus). In many cases separation of the species based on morphology alone is challenging. In general, tef1 distinguishes the species better than nrITS. Structured infraspecific genetic variation was detected in the nrITS phylogenies for five species (P. atromarginatus, P. hibbettii, P. orestes, P. primus and P. shikae) and in the tef1 phylogenies for P. cervinus. Phylogeographic patterns are strikingly different among the species in this group and include widespread Holarctic species, exclusively Palearctic, putative disjuncts and endemics in each Holarctic subregion (Eastern/Western Palearctic and Nearctic). Identification keys are provided for each subregion.

Taxonomy and phylogeny of Pluteus glaucotinctus sensu lato (Agaricales, Basidiomycota), a multicontinental species complex

“…P. Kumm. (Saupe 1981;Stijve & Bonnard 1986;Justo et al 2014), P. americanus (P. Banerjee & Sundb.) Justo, E.F. Malysheva & Minnis (Justo et al 2014) and P. saupei Justo & Minnis (Saupe 1981;Justo et al 2011b;Justo et al 2014).…”

“…(Saupe 1981;Stijve & Bonnard 1986;Justo et al 2014), P. americanus (P. Banerjee & Sundb.) Justo, E.F. Malysheva & Minnis (Justo et al 2014) and P. saupei Justo & Minnis (Saupe 1981;Justo et al 2011b;Justo et al 2014). Additionally, P. nigrolineatus Murrill is also frequently reported with blue-green pigments at the stipe base (Singer 1962;Rodríguez & Guzmán-Dávalos 2001) and recently Menolli et al (2010) showed by molecular data that this species is also positioned with other ones of the salicinus/albostipitatus clade.…”

“…Pluteus, most of which are represented by species complexes with apparent intercontinental distributions. Many of these have been investigated (Justo et al 2014) and others require further molecular and morphological studies to better understand species delimitation, diversity, and biogeography in the genus. In the present article we critically examine the widespread species complex around P. glaucotinctus using morphological approaches and molecular phylogenies based on two loci, the nuclear ribosomal internal transcribed spacers region (nrITS) and translation-elongation factor 1-alpha (tef1 a single-copy, protein-coding gene).…”

New information about agaricoid basidiomycetes of the Tigirek State Nature Reserve (Altai territory)

“…14 VIII 2016» (NSK 1003311). Ши-роко распространен в Евразии и восточной части Северной Америки (Justo et al, 2014 …”

The history of mycological studies in Khanty-Mansi autonomous okrug: 2) studies of Macromycetes, Lichens and Myxomycetes, state of mycological collections and fungal records database

“…nov. [Malysheva et al, 2007]. Последующий сбор коллекций этого рода внес вклад в подробную ревизию одной из секций рода [Justo et al, 2014]. Несколько образцов, собранных в районе Ханты-Мансийска, были использованы для описания двух новых видов (Pluteus rangifer sp.…”