Molecular phylogenies of Parabasalia inferred from four protein genes and comparison with rRNA trees

Gerbod, Delphine; Sanders, Emily; Moriya, Shigeharu; Noël, Christophe; Takasu, Hirotoshi; Fast, Naomi M.; Delgado‐Viscogliosi, Pilar; Ohkuma, Moriya; Kudo, Toshiaki; Capron, Moníque; Palmer, Jeffrey D.; Keeling, Patrick J.; Viscogliosi, Éric

doi:10.1016/j.ympev.2003.09.013

Cited by 43 publications

(29 citation statements)

References 37 publications

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“…The GAPDH sequence has been shown to be useful as a phylogenetic marker in Parabasalia despite the presence of multiple sequences in the genome (Gerbod et al 2004;Ohkuma et al 2007a). The GAPDH gene sequences were identified from T. acuta and E. imla in Cryptocercus with RT-PCR using the manually isolated cells.…”

Section: Resultsmentioning

confidence: 99%

“…The family-level groups (T: Trichonymphidae, E: Eucomonymphidae plus Teranymphidae) and the orders Spirotrichonymphida (S) and Cristamonadida (C) are indicated with vertical bars. The accession numbers of the other sequences have been reported in Gerbod et al (2004) and Ohkuma et al (2007a,b). Bayesian posterior probability and PHYML bootstrap value (divided by slash) are indicated in each node.…”

Section: Discussionmentioning

confidence: 99%

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Inheritance and diversification of symbiotic trichonymphid flagellates from a common ancestor of termites and the cockroachCryptocercus

et al. 2008

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Cryptocercus cockroaches and lower termites harbour obligate, diverse and unique symbiotic cellulolytic flagellates in their hindgut that are considered critical in the development of social behaviour in their hosts. However, there has been controversy concerning the origin of these symbiotic flagellates. Here, molecular sequences encoding small subunit rRNA and glyceraldehyde-3-phosphate dehydrogenase were identified in the symbiotic flagellates of the order Trichonymphida (phylum Parabasalia) in the gut of Cryptocercus punctulatus and compared phylogenetically to the corresponding species in termites. In each of the monophyletic lineages that represent family-level groups in Trichonymphida, the symbionts of Cryptocercus were robustly sister to those of termites. Together with the recent evidence for the sister-group relationship of the host insects, this first comprehensive study comparing symbiont molecular phylogeny strongly suggests that a set of symbiotic flagellates representative of extant diversity was already established in an ancestor common to Cryptocercus and termites, was vertically transmitted to their offspring, and subsequently became diversified to distinct levels, depending on both the host and the symbiont lineages.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Inheritance and diversification of symbiotic trichonymphid flagellates from a common ancestor of termites and the cockroachCryptocercus

et al. 2008

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“…the nematode Cooperia (58), the sea urchin Strongylocentrotus (59), the squid Loligo (60), the Antarctic fish Notothenia (61), Xenopus (62), the chicken (63), and the mouse (64)). However, Thr 429 is replaced by valine in cotton (65), by serine in the moss Physcomitrella (66), and by glycine in the platyhelminth Echinococcus (67), the parabasalid Trichonympha (68), and Giardia (69). Interestingly, in the human ␤VI isotype, Thr 429 is replaced by a lysine (National Center for Biotechnology accession number CAC09371).…”

Section: Discussionmentioning

confidence: 99%

Roles of β-Tubulin Residues Ala428 and Thr429 in Microtubule Formation in Vivo

Joe

Banerjee

Ludueña

2009

Journal of Biological Chemistry

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The C termini of ␤-tubulin isotypes are regions of high sequence variability that bind to microtubule-associated proteins and motors and undergo various post-translational modifications such as polyglutamylation and polyglycylation. Crystallographic analyses have been unsuccessful in resolving tubulin C termini. Here, we used a stepwise approach to study the role of this region in microtubule assembly. We generated a series of truncation mutants of human ␤I and ␤III tubulin.

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“…This is in contrast to the findings of Gerbod et al (2001), who found a closer relationship between H. meleagridis and T. foetus based on the rRNA gene. However, the beta-tubulin gene provides only a low phylogenetic resolution (Gerbod et al 2004;Ohkuma et al 2007), and we only used a part of it. The investigation of more suitable genes would be required to determine the phylogenetic position more reliably.…”

Section: Discussionmentioning

confidence: 99%

Partial sequence of the beta-tubulin of Histomonas meleagridis and the activity of benzimidazoles against H. meleagridis in vitro

Hauck

Hafez

2008

Parasitol Res

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The protozoan parasite Histomonas meleagridis is a member of the family Monocercomonadidae in the class Trichomonada. Due to food safety concerns, currently no prophylactic or therapeutic drug against the parasite is licensed in the European Union. Benzimidazoles are antiparasitic drugs, and some of them are licensed for use in food-producing animals. Benzimidazoles act on beta-tubulin, and the beta-tubulin sequence allows predictions about the efficacy of benzimidazoles. In this study, we sequenced and analyzed a part of the beta-tubulin gene of five H. meleagridis strains and of Dientamoeba fragilis. In each Histomonas strain, three to five different sequences were found. No clustering of sequences from the same strain was recognizable. A phylogenetic tree based on the amino acid sequences of trichomonal beta-tubulin genes placed the histomonal sequences on a branch with D. fragilis, separate from Monocercomonas sp. and Tritrichomonas foetus. All histomonal amino acid sequences predicted a susceptibility to benzimidazoles. However, when we tested the efficacy of five benzimidazoles, namely, albendazole, fenbendazole, flubendazole, mebendazole, and nocodazole, on H. meleagridis in vitro, all tested drugs showed no efficacy, even though the concentrations tested were higher than the concentrations found to be effective against Trichomonas vaginalis and T. foetus by other investigators.

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Molecular phylogenies of Parabasalia inferred from four protein genes and comparison with rRNA trees

Cited by 43 publications

References 37 publications

Inheritance and diversification of symbiotic trichonymphid flagellates from a common ancestor of termites and the cockroachCryptocercus

Inheritance and diversification of symbiotic trichonymphid flagellates from a common ancestor of termites and the cockroachCryptocercus

Roles of β-Tubulin Residues Ala428 and Thr429 in Microtubule Formation in Vivo

Partial sequence of the beta-tubulin of Histomonas meleagridis and the activity of benzimidazoles against H. meleagridis in vitro

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