Molecular phylogenetics of Trypanosomatidae: contrasting results from 18S rRNA and protein phylogenies

Hughes, Austin L.; Piontkivska, Helen

doi:10.1186/1475-9292-2-15

Cited by 32 publications

(7 citation statements)

References 21 publications

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“…This is additional evidence for the hypothesis supporting very close evolutionary relationship between Leishmania spp. and Crithidia spp [6] , [7] , [8] , [9] . Also, only 9.3% matched with Crithidia spp., as very few genes had been identified in this organism prior to the release of the reference genome sequence (Tables S1–S3 in S1 File ).…”

Section: Resultsmentioning

confidence: 99%

“…For this reason, their biology at the molecular and cellular levels remains almost unexplored despite their evolutionary relation with the genus Leishmania (reviewed in [6] ). Advantageously, both organisms are closely related at the crown of the phylogenetic tree of trypanosomatids [6] , [7] , [8] , [9] despite their different life cycles.…”

Section: Introductionmentioning

confidence: 99%

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An Insight into the Proteome of Crithidia fasciculata Choanomastigotes as a Comparative Approach to Axenic Growth, Peanut Lectin Agglutination and Differentiation of Leishmania spp. Promastigotes

et al. 2014

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The life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The comparison of these non-pathogenic microorganisms evolutionary close to other species of trypanosomatids that develop digenetic life cycles and cause chronic severe sickness to millions of people worldwide is of outstanding interest. A ground-breaking analysis of differential protein abundance in Crithidia fasciculata is reported herein. The comparison of the outcome with previous gene expression profiling studies developed in the related human pathogens of the genus Leishmania has revealed substantial differences between the motile stages of these closely related organisms in abundance of proteins involved in catabolism, redox homeostasis, intracellular signalling, and gene expression regulation. As L. major and L. infantum agglutinate with peanut lectin and non-agglutinating parasites are more infective, the agglutination properties were evaluated in C. fasciculata. The result is that choanomastigotes are able to agglutinate with peanut lectin and a non-agglutinating subpopulation can be also isolated. As a difference with L. infantum, the non-agglutinating subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Insight into the Proteome of Crithidia fasciculata Choanomastigotes as a Comparative Approach to Axenic Growth, Peanut Lectin Agglutination and Differentiation of Leishmania spp. Promastigotes

et al. 2014

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“…Hughes and Piontkivska, [28] classified trypanosome species according to amino acid sequence of small ribosomal subunit into 42 protein families. They also classified Trypanosoma families according to host and location into American, African and bonoidae.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt

El‒Sayed¹,

El‐Adl²,

Ali³

et al. 2020

Preprint

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Phylogenetic analysis of blood parasite infections including Babesia (B.) bovis, Babesia microti and Trypanosoma (T.) spp. in one-humped camel (Camelus dromedarius) (n= 142) breeds in Halayeb and Shalateen, in Upper Egypt were performed in the current study. Polymerase chain reaction (PCR) assays targeting the Rhoptry Associated Protein-1 (RAP-1), Babesia microti small subunit rRNA (ss-rRNA) and internal transcribed spacer 1 (ITS1) genes were used to detect the prevalence of B. bovis, B. microti and Trypanosoma spp. in camels, respectively. Nested PCR assays were used for the detection of Babesia spp. (B. bovis and B. microti). While, KIN-multi species PCR reaction was employed to detect and identify trypanosome DNA in camels. B. microti was detected in (17/142) with infection rate (11.97 %). Sequencing and phylogenetic analyses revealed that B. microti detected in camel was closely related to the German strain in rats and voles in France. B. bovis was also detected in (4/142) with infection rate (2.81%). The sequence and phylogenetic analyses revealed that the isolated B. bovis was closely related to strains isolated from Argentine, USA and Brazil. Moreover, T. evansi was detected in (8/142) with infection rate (5.63%). Sequence and phylogenetic analyses revealed that isolated T. evansi was closely related to T. theileri that was detected from cattle in Brazil. This study provides the first evidence of B. microti in camel in Egypt and highlights the possible role of one-humped camels in maintaining the enzootic cycle of Babesia transmission in Egypt.

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“…To compare CIFs between TriTrypDB genomes and humans and to have sufficient data to estimate trypanosome CIFs, we defined eight phylogenetic clades for 39 of the 46 trypanosome genomes as shown in Table 1. These clades were based on a composite of phylogenetic results in the literature [44][45][46][47]. CIFs were subsequently estimated for each clade independently, by pooling tRNA genes within clades.…”

Section: Prediction Of Divergent Trna Class-informative Features (Cifmentioning

confidence: 99%

Targeting tRNA-synthetase interactions towards novel therapeutic discovery against eukaryotic pathogens

et al. 2020

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The development of chemotherapies against eukaryotic pathogens is especially challenging because of both the evolutionary conservation of drug targets between host and parasite, and the evolution of strain-dependent drug resistance. There is a strong need for new nontoxic drugs with broad-spectrum activity against trypanosome parasites such as Leishmania and Trypanosoma. A relatively untested approach is to target macromolecular interactions in parasites rather than small molecular interactions, under the hypothesis that the features specifying macromolecular interactions diverge more rapidly through coevolution. We computed tRNA Class-Informative Features in humans and independently in eight distinct clades of trypanosomes, identifying parasite-specific informative features, including base pairs and base mis-pairs, that are broadly conserved over approximately 250 million years of trypanosome evolution. Validating these observations, we demonstrated biochemically that tRNA:aminoacyl-tRNA synthetase (aaRS) interactions are a promising target for antitrypanosomal drug discovery. From a marine natural products extract library, we identified several fractions with inhibitory activity toward Leishmania major alanyl-tRNA synthetase (AlaRS) but no activity against the human homolog. These marine natural products extracts showed cross-reactivity towards Trypanosoma cruzi AlaRS indicating the broad-spectrum potential of our network predictions. We also identified Leishmania major threonyl-tRNA synthetase (ThrRS) inhibitors from the same library. We discuss why chemotherapies targeting multiple aaRSs should be less prone to the evolution of resistance than monotherapeutic or synergistic combination chemotherapies targeting only one aaRS.

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Molecular phylogenetics of Trypanosomatidae: contrasting results from 18S rRNA and protein phylogenies

Cited by 32 publications

References 21 publications

An Insight into the Proteome of Crithidia fasciculata Choanomastigotes as a Comparative Approach to Axenic Growth, Peanut Lectin Agglutination and Differentiation of Leishmania spp. Promastigotes

An Insight into the Proteome of Crithidia fasciculata Choanomastigotes as a Comparative Approach to Axenic Growth, Peanut Lectin Agglutination and Differentiation of Leishmania spp. Promastigotes

Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt

Targeting tRNA-synthetase interactions towards novel therapeutic discovery against eukaryotic pathogens

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