Molecular phylogenetics of ascomycotal adhesins—A novel family of putative cell-surface adhesive proteins in fission yeasts

Linder, Tomas; Gustafsson, Claes M.

doi:10.1016/j.fgb.2007.08.002

Cited by 73 publications

(99 citation statements)

References 76 publications

(122 reference statements)

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“…To compare the sequence of the Flo11 domain, we searched and aligned homologous sequences. Similar to the result reported by Linder and Gustafsson (2008), the homology search did not detect a Flo11 domain in any other fungal lineages except Saccharomycotina. The phylogenetic analysis revealed that the Flo11 domain obtained above is similar to that of Flo11ap and Flo11bp, but some differences existed ( Figure S3).…”

Section: Identification Of the Gene Conferring Floatability To Z Rouxiisupporting

confidence: 87%

“…A recent study showed that the N-terminal domain of Flo11p, which is referred to as the Flo11 domain, self-interacts and explains the cell-cell interaction capacity of FLO11-expressing cells (Goossens and Willaert 2012). The Flo11 domain is conserved in the S. cerevisiae Flo11 protein and in the respective proteins from some related species within Saccharomycotina but was not found in any other fungal lineage (Linder and Gustafsson 2008). We searched for the FLO11 homolog in the Z. rouxii genome database (Sherman et al 2009) to identify the gene conferring floatability to Z. rouxii.…”

Section: Identification Of the Gene Conferring Floatability To Z Rouxiimentioning

confidence: 99%

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Adaptation of the Osmotolerant Yeast Zygosaccharomyces rouxii to an Osmotic Environment Through Copy Number Amplification of FLO11D

2013

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Copy number variations (CNVs) contribute to the adaptation process in two possible ways. First, they may have a direct role, in which a certain number of copies often provide a selective advantage. Second, CNVs can also indirectly contribute to adaptation because a higher copy number increases the so-called "mutational target size." In this study, we show that the copy number amplification of FLO11D in the osmotolerant yeast Zygosaccharomyces rouxii promotes its further adaptation to a florformative environment, such as osmostress static culture conditions. We demonstrate that a gene, which was identified as FLO11D, is responsible for flor formation and that its expression is induced by osmostress under glucose-free conditions, which confer unique characteristics to Z. rouxii, such as osmostress-dependent flor formation. This organism possesses zero to three copies of FLO11D, and it appears likely that the FLO11D copy number increased in a branch of the Z. rouxii tree. The cellular hydrophobicity correlates with the FLO11D copy number, and the strain with a higher copy number of FLO11D exhibits a fitness advantage compared to a reference strain under osmostress static culture conditions. Our data indicate that the FLO gene-related system in Z. rouxii has evolved remarkably to adapt to osmostress environments.A N organism adapts to adverse environments to survive and produce progeny. Adaptive evolution is the process through which a population becomes better suited to its environment via mutation, selection, and random drift (Bürger 2000;Cressman 2003;Ewens 2004;Nowak and Sigmund 2004). Types of mutations include nucleotide changes (Steiner et al. 2007;Barrick and Lenski 2009), transposition events (Wilke and Adams 1992;Aminetzach et al. 2005), and copy number variations (CNVs), including gene amplifications (duplication) and deletions (Brown et al. 1998;Perry et al. 2007;Gresham et al. 2010). CNVs can have a phenotypic impact and can consequently alter the fitness of an allele through the following mechanisms: (i) changing the coding sequence of a gene (Yamanaka et al. 2009;Schlattl et al. 2011), (ii) creating paralogs that can diverge from each other and take on new or specialized functions (neofunctionalization or subfunctionalization, respectively) (Ohno 1970;Innan and Kondrashov 2010), and (iii) altering the expression level of a gene (gene dosage effect) (Stranger et al. 2007;Yamanaka et al. 2009;Iskow et al. 2012). In humans, most CNVs overlapping genes are under purifying (negative) selection (Conrad et al. 2010), but a handful of CNVs are thought to be under positive selection (Cooper et al. 2007;Hurles et al. 2010;Iskow et al. 2012) such as AMY1 (Perry et al. 2007). In yeast, there are several examples of how the (increased) copy number can provide a direct selective advantage (Gresham et al. 2008;Voordeckers et al. 2012).In nature, budding yeast exhibits a number of adaptive responses, such as filamentation, invasive growth, flocculation, and biofilm formation, to overcome a deleterious enviro...

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Section: Identification Of the Gene Conferring Floatability To Z Rouxiisupporting

confidence: 87%

Section: Identification Of the Gene Conferring Floatability To Z Rouxiimentioning

confidence: 99%

Adaptation of the Osmotolerant Yeast Zygosaccharomyces rouxii to an Osmotic Environment Through Copy Number Amplification of FLO11D

2013

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“…Sexual flocculation is the result of specific recognition of cell surface proteins called "adhesion molecules" from one mating type by sugar molecules or amino acid residues on the surface of the opposite mating cell type (5). Several mating type-specific adhesins from fission yeast have been described (6,7). Adhesin genes are not constitutively expressed and are subjected to tight transcriptional regulation (8).…”

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confidence: 99%

Reduction of Ribosome Level Triggers Flocculation of Fission Yeast Cells

Sun

et al. 2013

Eukaryot Cell

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Deletion of ribosomal protein L32 genes resulted in a nonsexual flocculation of fission yeast. Nonsexual flocculation also occurred when two other ribosomal protein genes, rpl21-2 and rpl9-2, were deleted. However, deletion of two nonribosomal protein genes, mpg and fbp, did not cause flocculation. Overall transcript levels of rpl32 in rpl32-1⌬ and rpl32-2⌬ cells were reduced by 35.9% and 46.9%, respectively, and overall ribosome levels in rpl32-1⌬ and rpl32-2⌬ cells dropped 31.1% and 27.8%, respectively, compared to wild-type cells. Interestingly, ribosome protein expression levels and ribosome levels were also reduced greatly in sexually flocculating diploid YHL6381/WT (h ؉ /h ؊ ) cells compared to a mixture of YHL6381 (h ؉ ) and WT (h ؊ ) nonflocculating haploid cells. Transcriptome analysis indicated that the reduction of ribosomal levels in sexual flocculating cells was caused by more-extensive suppression of ribosomal biosynthesis gene expression, while the reduction of ribosomal levels caused by deleting ribosomal protein genes in nonsexual flocculating cells was due to an imbalance between ribosomal proteins. We propose that once the reduction of ribosomal levels is below a certain threshold value, flocculation is triggered.

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“…Amino acid sequences with homology to zonadhesin occur in peptidases and few other proteins of P. pastoris. 6 Adhesion molecules of bacteria and fungi are crucial for cell-cell interaction, adherence to surrounding tissues, and the virulence of pathogens (34). Zonadhesin and several other fungal adhesins contain a ligand binding N-terminal domain of 300 residues and a C-terminal domain with repeats of Thr, Ser, and hydrophobic residues (34,35).…”

Section: Discussionmentioning

confidence: 99%

“…The fold of the two domains with -sandwiches can be described as IgG-like (see below). The sequence of the missing C-terminal domain with 248 residues lists 64 Thr/Ser residues and 68 Gly/Ala/Val residues in characteristic repeat of adhesins (34,35).…”

Section: Overall Structure Of Zonadhesin and Its Interaction With Gg-mentioning

confidence: 99%

Crystal structure of linoleate 13R-manganese lipoxygenase in complex with an adhesion protein

Chen

Wennman

Karkehabadi

et al. 2016

Journal of Lipid Research

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Molecular phylogenetics of ascomycotal adhesins—A novel family of putative cell-surface adhesive proteins in fission yeasts

Cited by 73 publications

References 76 publications

Adaptation of the Osmotolerant Yeast Zygosaccharomyces rouxii to an Osmotic Environment Through Copy Number Amplification of FLO11D

Adaptation of the Osmotolerant Yeast Zygosaccharomyces rouxii to an Osmotic Environment Through Copy Number Amplification of FLO11D

Reduction of Ribosome Level Triggers Flocculation of Fission Yeast Cells

Crystal structure of linoleate 13R-manganese lipoxygenase in complex with an adhesion protein

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