Molecular Phylogenetics and Evolution of the Cytochrome b Gene in the Cyprinid Genus Lythrurus (Actinopterygii: Cypriniformes)

Schmidt, Timothy R.; Bielawski, Joseph P.; Gold, John R.

doi:10.2307/1447697

Cited by 62 publications

(40 citation statements)

References 37 publications

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“…The amount of divergence observed between those two populations is on the order of species-level differentiations noted in other studies of cyprinids (e.g. Schmidt et al 1998;McPhail and Taylor 1999;Zardoya and Doadrio 1999;Pfrender et al 2004). On the other hand, the values within-Han and within-Geum were not significant and relatively low, suggesting weak genetic structuring within drainages (cytochrome b, and control region; unpublished data).…”

Section: Resultssupporting

confidence: 56%

Characterization and pilot application of 14 microsatellite loci from the Korean shiner (Coreoleuciscus splendidus)

Kim

Ryu

et al. 2012

Animal Cells and Systems

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The Korean shiner (Coreoleuciscus splendidus, Gobioninae), a small freshwater fish native to Korea Peninsula, widely inhabits in most of the major drainages in South Korea. Here we describe the development of 14 novel and polymorphic microsatellites for this species and its effective utilization in estimation of genetic diversity using 72 individuals from three sampling sites in different drainages. Loci were isolated from a microsatellite enrichment procedure using probe-labeled magnetic beads. A total of 242 alleles were detected across all loci with an average of 17.3 alleles per locus ranging 4Á32. The loci varied levels of polymorphism as evident from its expected heterozygosity ranging from 0.111 to 0.957. The average pairwise F ST between two populations examined shows significant differentiation (F ST 00.215, p B 0.05). The 14 microsatellite loci developed here will also be useful to explain for the genetic structures among geographically isolated populations and gene flow dynamics within drainages in this species as well as the closely related species.

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Section: Resultssupporting

confidence: 56%

Characterization and pilot application of 14 microsatellite loci from the Korean shiner (Coreoleuciscus splendidus)

Kim

Ryu

et al. 2012

Animal Cells and Systems

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“…We sequenced mitochondrial NADH dehydrogenase subunit 2 (ND2) and Cytochrome b (Cyt b) genes and the intron 1 of the nuclear Hox c6a gene. PCR primers for ND2 were from Broughton and Gold (2000) and for Cyt b were from Schmidt et al (1998). We designed species-specific primers in several cases where apparent nucleotide substitutions in the priming site inhibited primer binding (available from R.E.B.…”

Section: Methodsmentioning

confidence: 99%

Current and historical hybridization with differential introgression among three species of cyprinid fishes (genus Cyprinella)

et al. 2011

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Hybridization is common among freshwater fishes, particular among the Cyprinidae. We used two mitochondrial genes and one nuclear gene to characterize hybridization among two species pairs of Cyprinella in southwestern North America. Genalogical patterns revealed that C. lutrensis and C. venusta are currently hybridizing in several localities producing apparent F(1), F(2) and backcross generations, yet there was no evidence for introgression outside of local hybrid zones. Alternatively, mitochondrial haplotypes from C. lutrensis appear to have introgressed into a C. lepida population in the Nueces River completely replacing the native C. lepida haplotype. There was no evidence of introgression of nuclear DNA and there does not appear to be ongoing hybridization. The population of C. lepida from the nearby Frio River exhibits no evidence of hybridization with C. lutrensis. Thus, contact between C. lutrensis and C. venusta results in the formation of localized hybrid swarms, while contact between C. lutrensis and C. lepida has resulted in complete mitochondrial introgression in the Nueces River or no apparent hybridization in the Frio River. The three different outcomes of contact between these species illustrate the variable nature of interspecific reproductive interactions and provide an excellent system in which to better understand the factors influencing hybridization among freshwater fishes.

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“…In some instances, we also used degenerate internal primers (forward, 5Ј-CTYAARATTGGNCTYGCYCCCCT-3Ј; backward, 5Ј-RATNGAKGTGAGKGCRGGGGC-3Ј) to obtain sequences originating around ND2 base positions 330 (forward sequence) and 700 (reverse sequence). Cytochrome b was amplified with primers LA and HA (Schmidt et al, 1998) in 50 l reactions with the following thermal profile (30 cycles): 94 C for 1 min, 48 C for 1 min, and 72 C for 2 min. The same primers were used to sequence the amplified gene with an ABI Prism 377 automated sequencer.…”

Section: Methodsmentioning

confidence: 99%

Historical Biogeography of the New-World Pupfish Genus Cyprinodon (Teleostei: Cyprinodontidae)

Echelle¹,

Carson²,

Echelle³

et al. 2005

Copeia

109

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Analysis of mtDNA sequence variation (2,548 bp from ND2, cytb, and part of the control region) indicates that the genus Cyprinodon began diverging in the Late Miocene from a common ancestor with Megupsilon, a monotypic genus on the Mesa del Norte of Mexico. The geographic pattern of mtDNA variation, with estimates of divergence time, suggests that by the end of the Miocene Cyprinodon occurred from the Atlantic Coast and West Indies to near the western margin of North America via ancestral Rio Grande and Colorado River systems. Phylogeographic structure within the major mtDNA complexes supports a variety of hypotheses from geology and previous phylogenetic analyses for Late Neogene connections among basins in southwestern North America now separated by formidable barriers to dispersal. Comparison of the mtDNA tree with previous phylogenetic inferences from allozymes indicates that reticulate evolution involving divergent lineages probably was important in the history of Cyprinodon.El análisis de variació n de secuencias de ADNmt (ND2, cytb, y parte de la región reguladora; 2,548 pb) indica que el género Cyprinodon empezó a divergir en el mioceno tardío de un antepasado comú n con Megupsilon, un género monotípico de la Mesa del Norte de México. El patró n geográfico de la variació n de ADNmt, con estimadas de los tiempos divergencias, sugiere que al llegar al fin del mioceno Cyprinodon ocurrió desde la costa Atlántica y las Antillas hasta casi el margen oeste de Norteamérica por los sistemas pluviales antiguos del río Bravo y del río Colorado. La estructura filogeográfica entre los grupos principales de ADNmt apoya a una variedad de hipó tesis de la geología y de los análisis filogenéticos anteriores para conexiones del neogeno tardío entre cuencas del suroeste de Norteamérica que ahora están separadas por barreras imponentes contra la dispersió n. La comparació n del árbol de ADNmt con deducciones filogenéticos anteriores de alozimas indica que la evolució n reticulada que incluye líneas divergentes probablemente fue importante en la historia del Cyprinodon.

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Molecular Phylogenetics and Evolution of the Cytochrome b Gene in the Cyprinid Genus Lythrurus (Actinopterygii: Cypriniformes)

Cited by 62 publications

References 37 publications

Characterization and pilot application of 14 microsatellite loci from the Korean shiner (Coreoleuciscus splendidus)

Characterization and pilot application of 14 microsatellite loci from the Korean shiner (Coreoleuciscus splendidus)

Current and historical hybridization with differential introgression among three species of cyprinid fishes (genus Cyprinella)

Historical Biogeography of the New-World Pupfish Genus Cyprinodon (Teleostei: Cyprinodontidae)

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