Molecular Phylogenetics and Biological Potential of Fungal Endophytes From Plants of the Sundarbans Mangrove

Rahaman, Sohanur; Siraj, Md Afjalus; Sultana, Syeda Sharmeen; Seidel, Véronique; Islam, Abu Zafor Md. Touhidul

doi:10.3389/fmicb.2020.570855

Cited by 19 publications

(14 citation statements)

References 72 publications

(78 reference statements)

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“…Moreover, fungal endophytes also are recognized as good sources of antioxidants as an alternative to plant extracts and synthetic antioxidants. There are numerous reports demonstrating antioxidant properties of fungi related to secondary metabolites like polyphenols and flavonoids that are plant-derived compounds with a variety of significant pharmacological activities (Kumar and Pandey 2013;Shahidi and Ambigaipalan 2015;Dhayanithy et al 2019;Rahaman et al 2020). Here, the extracts of bioactive strains subjected to four different in vitro antioxidant experiments, DPPH, hydroxyl radical, superoxide radical, and reducing power, revealed good antioxidant activities.…”

Section: Discussionmentioning

confidence: 84%

Distribution, cytotoxicity, and antioxidant activity of fungal endophytes isolated from Tsuga chinensis (Franch.) Pritz. in Ha Giang province, Vietnam

Pham

et al. 2022

Ann Microbiol

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Purpose An endangered Tsuga chinensis (Franch.) Pritz. is widely used as a natural medicinal herb in many countries, but little has been reported on its culturable endophytic fungi capable of producing secondary metabolites applied in modern medicine and pharmacy. The present study aimed to evaluate the distribution of fungal endophytes and their cytotoxic and antioxidant properties. Methods This study used the surface sterilization method to isolate endophytic fungi which were then identified using morphological characteristics and ITS sequence analysis. The antimicrobial and cytotoxic potentials of fungal ethyl acetate extracts were evaluated by the minimum inhibitory concentration (MIC) and sulforhodamine B (SRB) assays, respectively. Paclitaxel-producing fungi were primarily screened using PCR-based molecular markers. Additionally, biochemical assays were used to reveal the antioxidant potencies of selected strains. Results A total of sixteen endophytic fungi that belonged to 7 known and 1 unknown genera were isolated from T. chinensis. The greatest number of endophytes was found in leaves (50%), followed by stems (31.3%) and roots (18.7%). Out of 16 fungal strains, 33.3% of fungal extracts showed significant antimicrobial activities against at least 4 pathogens with inhibition zones ranging from 11.0 ± 0.4 to 25.8 ± 0.6 mm. The most prominent cytotoxicity against A549 and MCF7 cell lines (IC50 value < 92.4 μg/mL) was observed in Penicillium sp. SDF4, Penicillium sp. SDF5, Aspergillus sp. SDF8, and Aspergillus sp. SDF17. Out of three key genes (dbat, bapt, ts) involved in paclitaxel biosynthesis, strains SDF4, SDF8, and SDF17 gave one or two positive hits, holding the potential for producing the billion-dollar anticancer drug paclitaxel. Furthermore, four bioactive strains also displayed remarkable and wide-range antioxidant activity against DPPH, hydroxyl radical, and superoxide anion, which was in relation to the high content of flavonoids and polyphenols detected. Conclusion The present study exploited for the first time fungal endophytes from T. chinensis as a promising source for the discovery of new bioactive compounds or leads for the new drug candidates.

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Section: Discussionmentioning

confidence: 84%

Distribution, cytotoxicity, and antioxidant activity of fungal endophytes isolated from Tsuga chinensis (Franch.) Pritz. in Ha Giang province, Vietnam

Pham

et al. 2022

Ann Microbiol

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“…Salinity, water temperature, dissolved oxygen, and pH in seawater do not strongly affect growth of fungal communities, even of mycelia resting over long periods (Mouton et al, 2012;Manohar & Raghukumar, 2013;Rédou et al, 2015;Wijesekera et al, 2017;Grossart et al, 2019;Rojas-Jimenez et al, 2020). Fungi have been discovered in practically every marine ecosystem studied (Gladfelter et al, 2019;Grossart et al, 2019), including deep subsurface sediments (Rédou et al, 2014;Gladfelter et al, 2019;Vargas-Gastélum & Riquelme, 2020), coastal plankton (Taylor & Cunliffe, 2016), wood (Rämä et al, 2016;Lee et al, 2019;Rahaman et al, 2020), and marine animals (Wainwright et al, 2017;Amend et al, 2019) at depths ranging from the deep sea to surface waters (Amend et al, 2019). In addition, ascomycetes species are generally considered to be more tolerant of extreme environments (Rämä et al, 2016;Vargas-Gastélum & Riquelme, 2020).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Relationships, Phylogeographic Patterns, and a Hypothesis for Air- and Sea-driven Global Spread of Chromoblastomycosis by Etiological Fonsecaea Agents

Wang

Hao²,

Cai

et al. 2022

Preprint

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Fonsecaea spp. is the most common pathogen underlying chromoblastomycosis. However, many aspects of its evolutionary history are not fully understood, and a timescale for its diversification is lacking . Here, we propose, explain, and validate the evolutionary relationships, transmission routes, and modes of transmission among pathogenic species of the genus Fonsecaea . we investigated the sequences of a total of 510 intra-ribosomal transcribed regions and 485 reported cases of eight strains of the genus Fonsecaea , that have spread epidemically worldwide, persisted, and diversified over 38.27 million years. This period coincides with the diversification of many filamentous fungi, and this evolution explains the diversity and origin of pathogenic species of Fonsecaea spp . , as well as the possible transmission routes. Fonsecaea pedrosoi originated in the Atlantic Ocean near Brazil, F. monophora originated in the Pacific Ocean near southern China, and F. nubica originated in the Indian Ocean near Madagascar. F. pugnacius originated and is endemic in the Atlantic Ocean near Brazil. We proposed the hypothesis that non-pathogenic environmental strains in the environment ( Fonsecaea brasiliensis , F. multimorphosa , F. minima , and F. erecta ) evolved into pathogenic strains ( F. pedrosoi , F. pugnacius , F. nubica , and F. monophora ) and spread globally with global tropical cyclone and ocean currents pathways. F. multimorphosa is potentially infectious to humans. The present study’s results will greatly facilitate the prevention, treatment, and cure of Fonsecaea infections and give insight and reference to researchers in the field of fungal research, especially other pathogenic fungi and environmental fungi.

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“…In the current study, the number of isolates of P. chrysogenum were comparatively low. Earlier studies have reported this species as an endophyte in different mangrove plants (Devi, 2012, Rahaman, 2020. Penicillium citrinum is a lamentous fungus, commonly distributed world-wide and isolated from different sources such as soil, plants and indoor environments (Houbraken, 2010) and is a source of citrinin, a potent mycotoxin.…”

Section: Discussionmentioning

confidence: 99%

Diversity and bioactive profile of endophytic mycoflora in mangroves

Revathy

Mohan

Kesavan

et al. 2022

Preprint

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The present study deals with the mangrove associated endophytic fungi and their bioactivity profile. The fungal endophytes were isolated from the leaves of three mangrove species (Aegiceras corniculatum, Lumnitzera racemosa and Ceriops tagal) and identified by morphological and molecular methods (Internal Transcribed Spacer and β-tubulin (Ben A) sequencing). The isolates mainly belonged to Ascomycota (95%) and the Basidiomycota comprised only 5%. They come under 5 classes, 11 orders, 16 genera and 25 species. Under Ascomycota, four classes, viz., Sordariomycetes (44.5%), Eurotiomycetes (32.46%), Dothideomycetes (17.28%) and Saccharomycetes (1.83%) comprising 10 orders were recorded. Under Basidiomycota a single class viz., Agaricomycetes (3.93%) comprising 1 order (Agaricales) was observed. Colletotrichum (30.2%) was the dominant genus followed by Aspergillus (17.8%), Penicillium (14.1%), Phyllosticta (9.1%) etc. Penicillium citrinum was the dominant fungus (22%) in A. corniculatum; Colletotrichum siamense (31%) in L. racemosa and Aspergillus sydowii (35%) in C. tagal (Fig. 3). Among the isolates, the Colonization frequency (CF%) was maximum for Colletotrichum siamense (18%) followed by Phyllosticta capitalensis (11.6%). Penicillium citrinum was found in all the three plant species. Isolates from Ceriops tagal were found to exhibit higher antibacterial as well as hydrolytic enzyme production potential. Aspergillus montevidensis EF 30, Cladosporium oxysporum EF 316, Colletotrichum siamense EF 272, Penicillium chrysogenum EF 362, Colletotrichum fruticola EF 22, Ascotricha chartarum EF 374, Aspergillus sydowii EF 239, Talaromyces purpurogenus EF 313, Meyerozyma caribbica EF 347 and Schizophyllum commune EF 309 were segregated as potential strains for hydrolytic enzyme production. A. aculeatus EF 10, T. purpurogenus EF 313, C. oxysporum EF 316, C. fruticola EF 74, P. capitalensis EF 187, A. sydowi EF 226 and P. chrysogenum EF 363 showed notable antimicrobial property. T purprogenus EF 313 endowed with pigment (red) production has potential for application in dyeing industry. During the current study, 25 different species of endophytic fungal species could be recovered from three host mangrove plants and some of them were having high bioactive potential for possible commercial applications with respect to bioremediation and as antimicrobials in medicine and aquaculture.

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Molecular Phylogenetics and Biological Potential of Fungal Endophytes From Plants of the Sundarbans Mangrove

Cited by 19 publications

References 72 publications

Distribution, cytotoxicity, and antioxidant activity of fungal endophytes isolated from Tsuga chinensis (Franch.) Pritz. in Ha Giang province, Vietnam

Distribution, cytotoxicity, and antioxidant activity of fungal endophytes isolated from Tsuga chinensis (Franch.) Pritz. in Ha Giang province, Vietnam

Evolutionary Relationships, Phylogeographic Patterns, and a Hypothesis for Air- and Sea-driven Global Spread of Chromoblastomycosis by Etiological Fonsecaea Agents

Diversity and bioactive profile of endophytic mycoflora in mangroves

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