Molecular phylogenetic analysis of theAmiota apodemataandAmiota sinuataspecies groups (Diptera: Drosophilidae), with descriptions of four new species

Abstract

The phylogenetic relationships among the East Asian species of the apodemata and sinuata species groups of the genus Amiota were investigated based on DNA sequences of the mitochondrial NADH dehydrogenase subunit 2 (ND2) gene. A total of 23 samples of 12 species were employed as in‐group taxa, and one sample for each of four other Amiota species were used as out‐groups. The results suggested with strong confidence the monophyly of both the apodemata and the sinuata groups, whereas the monophyly of the ‘apodemata group + sinuata group’ cluster was less supported. Based on its geographical distribution, the origin of the sinuata group is supposed to be southern China. Four new species were described from Guangxi and Yunnan, China: Amiota reikae Xu & Chen sp. nov., Amiota guiensis Xu & Chen sp. nov., Amiota hesongensis Xu & Chen sp. nov., and Amiota polytreta Xu & Chen sp. nov. © 2013 The Linnean Society of London

Phylogeny of the Genus Drosophila

“…They and their students have contributed much to our understanding of relationships among the early diverging lineages in the Steganinae ( e.g. , Chen et al 2004 ; Zhao et al 2009 , 2013 ; Li et al 2014 ).…”

Revision of the Nearctic Species of the Genus Amiota Loew (Diptera: Drosophilidae)

“…Phylogenetic construction using sequence data can clarify many relationships, not just within Amiota, but among Steganinae genera broadly (O'Grady and DeSalle, 2018) , 1997). Points represent specimens of Amiota used in this stud the genus have been almost exclusively carried out on Chinese species with morphology (Chen and Toda, 2001) or only one to two gene regions (He et al, 2009;Otranto et al, 2008;Shao et al, 2014;Wang et al, 2020;Zhao et al, 2013). Molecular phylogenetics will help to interpret patterns of species distribution and endemism.…”

“…There is certainly less interest in studying flies with a relatively distant relationship to Drosophila. Molecular studies within the genus have focused on particular species groups within the Palaearctic fauna and were usually based on one gene region (Otranto et al, 2008;He et al, 2009;Zhao et al, 2013;Shao et al, 2014;Wang et al, 2020). Otranto et al (2008) was notable as the first molecular study with wide sampling within the subfamily, albeit with just COI regions.…”

Integrative taxonomy of the genus Pseudostegana (Diptera, Drosophilidae) from China, with descriptions of eleven new species

“…The COI and ND2 genes are useful for species identification or discrimination of Drosophilidae species (He et al, 2009; Zhao, Gao & Chen, 2009; Lu et al, 2011; Lu, Li & Chen, 2011; Zhao et al, 2013; Shao et al, 2014; Zhang, Li & Chen, 2016). Our results show that COI barcoding successfully distinguished all the studied Pseudostegana species and helped to discover the potential new species.…”