Molecular phylogenetic analysis of Fasciola flukes from eastern India

Ichikawa‐Seki, Madoka; Mohanta, Uday Kumar; Singh, T Shantikumar; Shoriki, Takuya; Sugiyama, Hiromu; Itagaki, Tadashi

doi:10.1016/j.parint.2015.04.004

Cited by 34 publications

(47 citation statements)

References 22 publications

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“…taxa in South West of Asia, in India and Bangladesh, near the studied region of Iran, but The Fg type of these flukes was detected in aspermic Fasciola sp. using ITS-RFLP, and also the phylogenetic study with ND1 gene showed that they were placed in F. gigantica complex (8,9). These flukes were probably considered as abnormal F. gigantica with oligozoospermia, which might have occurred because of the aging of flukes.…”

Section: Discussionmentioning

confidence: 93%

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Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Rouhani

Raeghi

Harandi

et al. 2017

Arch Clin Infect Dis

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Background: Fascioliasis is a neglected zoonotic disease with worldwide distribution. Phylogenetic analysis of Fasciola species is critical because of their different epidemiology. Objectives: The current study aimed at identifying Fasciola spp. based on spermatogenesis and phylogenetic analysis of mitochondrial (ND1) gene. Methods: One hundred and fifty adults with fascioliasis were selected from the Eastern provinces of Iran, based on spermatogenetic ability, measurement criteria, and ITS (nuclear ribosomal DNA internal transcribed spacers) 1 gene restriction fragment length polymorphism (RFLP) pattern. Then, their genetic diversity indices and phylogenetic relationships were analyzed using mitochondrial DNA marker of Fasciola population spread in the East of Iran. Results: Aspermic F. gigantica was observed in the eastern part of Iran. Partial sequences of mtDNA showed new haplotypes in both species. Pairwise fixation index between different F. gigantica populations calculated from the nucleotide data set of ND1 gene were statistically significant and showed the genetic differences in pairwise population. Conclusions:The results of the study showed that F. hepatica, lives in this region of Iran, had different genetic structures compared with the other Fasciola populations in the world. Fasciola gigantica present in the East of Iran had different genetic structures, compared with the other Fasciola population, based on genetic index.

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Section: Discussionmentioning

confidence: 93%

“…Morphological criteria such as body size and shape are among the traditional and important methods to distinguish between the 2 species, but these methods are not commonly trusted because of the variable range in different species (6 (8,9).…”

Section: Introductionmentioning

confidence: 99%

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Rouhani

Raeghi

Harandi

et al. 2017

Arch Clin Infect Dis

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show abstract

“…The Fasciola flukes were fixed in 70% ethanol and transported to the laboratory for further study. The seminal vesicle was removed from each fluke under a stereomicroscope, stained with hematoxylincarmine solution, and observed under an optical microscope for the presence of sperm [4,7].…”

Section: Collection Of Fasciola Flukes and Analysis Of Their Spermatomentioning

confidence: 99%

“…These two species have mature spermatozoa in their seminal vesicles, which undergo fertilization in the bile ducts of the host [4]. In addition to the two species, aspermic Fasciola flukes, which have few or no spermatozoa in their seminal vesicles, have been reported in Asian countries [4,7]. Recently, novel nuclear markers, the phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold) genes, have been used for the precise discrimination of F. hepatica, F. gigantica, and aspermic Fasciola flukes [5], which can be clearly distinguished with a multiplex polymerase chain reaction (PCR) and/or a PCR-restriction fragment length polymorphism method.…”

Section: Introductionmentioning

confidence: 99%

“…Fasciola gigantica is thought to be the predominant species in Southeast Asia [10][11][12]. A previous study based on the nad1 gene showed that the artificial movement of domestic ruminants, including zebu cattle and water buffaloes, has been involved in the spread of F. gigantica in Southeast Asian countries [7]. However, molecular information on the Fasciola flukes in Indonesia is limited till date.…”

Section: Introductionmentioning

confidence: 99%

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