Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips

Ahuja, Sangeeta; Mandal, B. B.; Dixit, Sonali; Srivastava, Prem S.

doi:10.1016/s0168-9452(02)00246-7

Cited by 50 publications

(21 citation statements)

References 10 publications

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“…and Gal. ; Ahuja et al 2002), black cosmos (Cosmos astrosanguineus (Hook.) Voss; Wilkinson et al 2003) and silver birch (Betula pendula Roth; Ryynanen and Aronen 2005).…”

Section: Morphological Analysismentioning

confidence: 99%

“…For example, growth rate of rice (Oryza sativa L.) and potato (Solanum tuberosum L.) plants recovered from cryopreserved tissues was found to be lower than that observed in plants from non-cryopreserved tissues (Moukadiri et al 1999;Harding and Staines 2001). However, in many other cases, no such changes in growth rates were observed (Ahuja et al 2002;Wilkinson et al 2003;Ryynanen and Aronen 2005). Karyotype studies showed that cryopreservation rarely inXicts gross changes in ploidy level (Hao et al 2002;Helliot et al 2002;Ryynanen and Aronen 2005;Urbanova et al 2002Urbanova et al , 2006.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos does not induce morphological, cytological or molecular changes in recovered seedlings

Sisunandar

Rival²,

Turquay³

et al. 2010

Planta

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The present study aimed at exploring the fidelity of coconut (Cocos nucifera L.) plants recovered from cryopreservation. Zygotic embryos from various different cultivars were cryopreserved following four successive steps, namely: rapid dehydration, rapid freezing, rapid thawing and in vitro recovery followed by acclimatization. At the end of the acclimatization period, the seedlings were compared to counterparts of the same age, which were produced from non-cryopreserved embryos. Both series were submitted to morphological, cytological and molecular comparisons. No significant differences in terms of growth rates could be measured. In addition, no morphological variation could be detected through the measurement of shoot elongation rates, production of opened leaves, and the number and total length of primary roots. Karyotype analysis revealed the same chromosome number (2n = 32) in all studied cultivars independently of cryopreservation. No significant differences could be observed between control and cryopreserved material concerning the type of chromosomes, the length of the long and short arms, the arm length ratio and the centromeric index. However, idiogram analysis did show a greater number of black banding on chromosomes isolated from cryopreserved material. Genetic and epigenetic fidelity was assessed through microsatellite (SSR) analysis and global DNA methylation rates; no significant differences would be observed between genomic DNAs isolated from seedlings originating from cryopreserved embryos and respective controls. In conclusion, our results suggest that the method of cryopreservation under study did not induce gross morphological, genetic or epigenetic changes, thus suggesting that it is an appropriate method to efficiently preserve coconut germplasm.

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“…and Gal. ; Ahuja et al 2002), black cosmos (Cosmos astrosanguineus (Hook.) Voss; Wilkinson et al 2003) and silver birch (Betula pendula Roth; Ryynanen and Aronen 2005).…”

Section: Morphological Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos does not induce morphological, cytological or molecular changes in recovered seedlings

Sisunandar

Rival²,

Turquay³

et al. 2010

Planta

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show abstract

“…Many reports have shown that cell and organ cultures regenerated after cryopreservation retain characteristics identical to those of the original cell lines with respect to DNA polymorphisms (Ahuja et al 2002;Hao et al 2002;Liu et al 2004), cytogenetic properties, (Urbanová et al 2002), transgene expression (Ryynänen et al 2002), and biochemical properties (Yoshimatsu et al 1996;Jung et al 2001). However, our knowledge of the influence of cryopreservation on the genetic stability of plant cells is still limited.…”

Section: Discussionmentioning

confidence: 99%

Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures

Kobayashi

Niino

Kobayashi

2005

Plant Biotechnology

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Tobacco BY-2 cells were successfully cryopreserved by a simple slow prefreezing (equilibrium freezing) method using an encapsulation technique. After the cells were immobilized in alginate gel beads, the beads were treated with cryoprotectant solution (2 M glycerol, 0.4 M sucrose) for 45 min. The beads were then transferred to a laboratory freezer at Ϫ30°C, stored for 2 h, and then immersed in liquid nitrogen. To initiate the regrowth of cells, the beads were warmed in a water bath. Following dilution of cryoprotectant solution, the beads were suspended and cultured in normal medium. With this method, suspension cell cultures were regrown within 7 days. There were no differences in the morphology or growth profiles between cryopreserved cell cultures and the original cell cultures.

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“…However, differences in band intensities among some but not all bands might indicate structural rearrangements in DNA caused by different types of DNA damage (Danylchenko and Sorochinsky, 2005) that might not be readily detected using the given system. The RAPD technology has previously been used successfully to detect occurrence of genetic alterations (Finkle et al, 1985;Harding, 1997;Aronen et al, 1999;Ahuja et al, 2002;Urbanova´ et al, 2005;Castillo et al, 2010), but this approach possesses limits with reproducibility, and it is currently being replaced by techniques such as Amplified Fragment Length Polymorphisms (AFLPs) and/or Simple Sequence Repeats (SSRs, microsatellites). These techniques are now being used to consider more carefully the issue of genetic fidelity after cryo-procedures, especially in the breeding of long-living conifers (Salaj et al, 2010), where genetic changes might be substantially expressed only later on, in mature trees.…”

Section: Variation In Cryopreserved Derived Plantmentioning

confidence: 99%

Fundamental Cryobiology and Basic Physical, Thermodynamical and Chemical Aspects of Plant Tissue Cryopreservation

Zeliang¹,

Pattanayak²

2012

Plant Breeding

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Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips

Cited by 50 publications

References 10 publications

Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos does not induce morphological, cytological or molecular changes in recovered seedlings

Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos does not induce morphological, cytological or molecular changes in recovered seedlings

Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures

Fundamental Cryobiology and Basic Physical, Thermodynamical and Chemical Aspects of Plant Tissue Cryopreservation

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