Molecular pathology of breast cancer

Abstract

The multistep model of carcinogenesis in the breast suggests a transition from normal epithelium to invasive carcinoma via non-atypical and atypical hyperplasia and in situ carcinoma. The introduction of mammographic screening has led to the increased detection of preinvasive disease, and this has highlighted deficiencies in the biology and classification of such lesions. The excitement surrounding the development of DNA microarray analysis and proteomics has raised expectations about the role of these techniques in understanding the biology and translating these data to clinical practice. Only a few years ago, scientists studied disease initiation and progression in a linear fashion, identifying and examining one cancer-related molecule at a time. The recent development of technologies that allow a large number of genes and gene products to be analysed simultaneously has brought renewed interest to breast cancer research, with the hope of identifying a unique 'fingerprint' for each tumour and hence individualised treatment. To date, histopathological assessment has been at the heart of clinical management-does the new technology herald the end? Hormone replacement therapy (HRT): indications and side effects

Targeting the effect of sofosbuvir on selective oncogenes expression level of hepatocellular carcinoma Ras/Raf/MEK/ERK pathway in Huh7 cell line

“…The most common mutation in this gene is V600E type of mutation which is frequently been identified in most types of cancers. However, this mutation has not proved to be linked with hepatocellular carcinoma ( Lakhani and Fox, 2017 ). The expression of B-RAF decreased slightly but the Ct value of the treated sample was still sufficient to upregulate the gene.…”