Molecular Pathogenesis of the Coronin Family: CORO2A Facilitates Migration and Invasion Abilities in Oral Squamous Cell Carcinoma

Kase-Kato, Ikuko; Asai, Shunichi; Minemura, Chikashi; Tsuneizumi, Kenta; Oshima, Sachi; Koma, Ayaka; Kasamatsu, Atsushi; Hanazawa, Toyoyuki; Uzawa, Katsuhiro; Seki, Naohiko

doi:10.3390/ijms222312684

Cited by 3 publications

(3 citation statements)

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“…The miRNA precursors, negative control miRNA, and siRNAs were obtained from Applied Biosystems (Waltham, MA, USA). The procedures used for the transient transfection of miRNAs, siRNAs, and plasmid vectors were described in our previous studies [ 20 , 21 , 57 , 58 ]. miRNAs at 10 nM and siRNAs at 5 nM were transfected into HNSCC cell lines using RNAiMAX reagent (Invitrogen, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The XTT assay (Sigma–Aldrich, St. Louis, MO, USA) characterized cell proliferation, a chamber assay using the Corning BioCoat TM cell culture chamber (Corning, Corning, NY, USA) assessed migration, and Matrigel chamber assays using the Corning BioCoat Matrigel assessed invasion. They were performed with HNSCC cells as described previously [ 20 , 21 , 57 , 58 ]. The reagents used in this study are listed in Supplemental Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…RNA extraction from cell lines and qRT-PCR were performed as described in our previous studies [ 20 , 21 , 57 , 58 ]. The TaqMan assays used in this study are summarized in Supplemental Table S1 .…”

Section: Methodsmentioning

confidence: 99%

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Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell Carcinoma

Minemura

Asai

Koma

et al. 2022

IJMS

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Recently, our studies revealed that some passenger strands of microRNAs (miRNAs) were closely involved in cancer pathogenesis. Analysis of miRNA expression signatures showed that the expression of miR-30e-3p (the passenger strand of pre-miR-30e) was significantly downregulated in cancer tissues. In this study, we focused on miR-30e-3p (the passenger strand of pre-miR-30e). We addressed target genes controlled by miR-30e-3p that were closely associated with the molecular pathogenesis of head and neck squamous cell carcinoma (HNSCC). Ectopic expression assays demonstrated that the expression of miR-30e-3p attenuated cancer cell malignant phenotypes (e.g., cell proliferation, migration, and invasive abilities). Our analysis of miR-30e-3p targets revealed that 11 genes (ADA, CPNE8, C14orf126, ERGIC2, HMGA2, PLS3, PSMD10, RALB, SERPINE1, SFXN1, and TMEM87B) were expressed at high levels in HNSCC patients. Moreover, they significantly predicted the short survival of HNSCC patients based on 5-year overall survival rates (p < 0.05) in The Cancer Genome Atlas (TCGA). Among these targets, SERPINE1 was found to be an independent prognostic factor for patient survival (multivariate Cox regression; hazard ratio = 1.6078, p < 0.05). Aberrant expression of SERPINE1 was observed in HNSCC clinical samples by immunohistochemical analysis. Functional assays by targeting SERPINE1 expression revealed that the malignant phenotypes (e.g., proliferation, migration, and invasion abilities) of HNSCC cells were suppressed by the silencing of SERPINE1 expression. Our miRNA-based approach will accelerate our understanding of the molecular pathogenesis of HNSCC.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell Carcinoma

Minemura

Asai

Koma

et al. 2022

IJMS

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CORO2A is a pan-cancer prognostic biomarker and correlates with immune infiltration

Xie

Wang

Zhu

et al. 2023

Preprint

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Background.Coronin 2A (CORO2A) is a member of the coronin family and reportedly functions as an oncogene in certain malignancies, although its correlation with prognosis and immune infiltration in different cancers remains unclear. Methods.Data were collected from the University of California Santa Cruz (UCSC), Human Protein Atlas (HPA), Tumor Immune Estimation Resource (TIMER), Tumor-Immune System Interactions (TISIDB) and Gene Set Enrichment Analysis (GSEA) databases. The differential expression of CORO2A, survival, clinical parameters, tumor mutational burden (TMB), microsatellite instability (MSI), mismatch repair (MMR) genes, DNA methyltransferases (DNMTs), tumor microenvironment (TME), immune-related genes (IRGs), immune infiltration, pathways and functions were analyzed using the R language software. Results.CORO2A was overexpressed in various malignancies, and correlated with clinical parameters, overall survival, disease-specific survival and progression-free survival in certain cancers. Furthermore, CORO2A was significantly correlated to the TMB, MSI, MMR genes, DNMTs, immune and stromal scores, IRGs and immune infiltration. GSEA further showed that CORO2A was associated with various immune-related pathways and functions in different cancer types. Conclusion.CORO2A is a promising prognostic and immunological marker for human cancers.

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Impact of miR-1/miR-133 Clustered miRNAs: PFN2 Facilitates Malignant Phenotypes in Head and Neck Squamous Cell Carcinoma

et al. 2022

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Based on our original RNA sequence-based microRNA (miRNA) signatures of head and neck squamous cell carcinoma (HNSCC), it was revealed that the expression levels of miR-1-3p, miR-206, miR-133a-3p, and miR-133b were significantly suppressed in cancer specimens. Seed sequences of miR-1-3p/miR-206 and miR-133a-3p/miR-133b are identical. Interestingly, miR-1-3p/miR-133a-3p and miR-206/miR-133b are clustered in the human genome. We hypothesized that the genes coordinately controlled by these miRNAs are closely involved in the malignant transformation of HNSCC. Our in silico analysis identified a total of 28 genes that had putative miR-1-3p/miR-133a-3p and miR-206/miR-133b binding sites. Moreover, their expression levels were upregulated in HNSCC tissues. Multivariate Cox regression analyses showed that expression of PFN2 and PSEN1 were independent prognostic factors for patients with HNSCC (p < 0.05). Notably, four miRNAs (i.e., miR-1-3p, miR-206, miR-133a-3p, and miR-133b) directly bound the 3′untranslated region of PFN2 and controlled expression of the gene in HNSCC cells. Overexpression of PFN2 was confirmed in clinical specimens, and its aberrant expression facilitated cancer cell migration and invasion abilities. Our miRNA-based strategy continues to uncover novel genes closely involved in the oncogenesis of HNSCC.

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Molecular Pathogenesis of the Coronin Family: CORO2A Facilitates Migration and Invasion Abilities in Oral Squamous Cell Carcinoma

Cited by 3 publications

References 57 publications

Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell Carcinoma

Identification of Tumor-Suppressive miR-30e-3p Targets: Involvement of SERPINE1 in the Molecular Pathogenesis of Head and Neck Squamous Cell Carcinoma

CORO2A is a pan-cancer prognostic biomarker and correlates with immune infiltration

Impact of miR-1/miR-133 Clustered miRNAs: PFN2 Facilitates Malignant Phenotypes in Head and Neck Squamous Cell Carcinoma

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