Molecular passportization of clones of karelian birch using PCRwith semi-specific primers

Abstract

Using 4 clones of Karelian birch (Betula pendula Roth var caretica Merckl.) from the collection of the karelian birch of the laboratory of genetics of Research Institute of Forest Genetics and Breeding, Voronezh, one normal tree of Betula pendula Roth, and one tree of B. pubescens Ehrh., collected from the nature, we have analyzed the possibility of application of PCR with semi-specific primers for molecular typing. We found primers with high percentage of polymorphic markers. These primers could be recommended for molecular typing of birch.