Molecular organization and mechanical properties of the hyaluronan matrix surrounding the mammalian oocyte

Salustri, Antonietta; Campagnolo, Luisa; Klinger, Francesca Gioia; Camaioni, Antonella

doi:10.1016/j.matbio.2018.02.002

Cited by 31 publications

(21 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ECM organized by cumulus cells around the mammalian oocyte during oocyte maturation is essential for oocyte release from the follicle, its transfer to the oviduct, and for sperm migration and fertilization [3,25,26]. In the present study, we demonstrate for the first time that the cumulus ECM contains two types of DPEAAE-positive G1 fragments derived from versican 1 (VG1): one is specific to cumulus cells while the other is in common with granulosa cells.…”

Section: Discussionmentioning

confidence: 61%

Versican G1 Fragment Establishes a Strongly Stabilized Interaction with Hyaluronan-Rich Expanding Matrix during Oocyte Maturation

Nagyová

Salustri

Němcová

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

In the mammalian ovary, the hyaluronan (HA)-rich cumulus extracellular matrix (ECM) organized during the gonadotropin-induced process of oocyte maturation is essential for ovulation of the oocyte-cumulus complex (OCC) and fertilization. Versican is an HA-binding proteoglycan that regulates cell function and ECM assembly. Versican cleavage and function remain to be determined in ovarian follicle. We investigated versican expression in porcine ovarian follicles by real-time (RT)-PCR and western blotting. The aims of the present work were to determine whether 1) versican was produced and cleaved by porcine OCCs during gonadotropin stimulation; 2) these processes were autonomous or required the participation of mural granulosa cells (MGCs); and 3) versican cleavage was involved in the formation or degradation of expanded cumulus ECM. We demonstrate two cleavage products of G1 domain of versican (V1) accumulated in the HA-rich cumulus ECM. One of them, a G1-DPEAAE N-terminal fragment (VG1) of ~70 kDa, was generated from V1 during organization of HA in in vivo and in vitro expanded porcine OCCs. Second, the V1-cleaved DPEAAE-positive form of ~65 kDa was the only species detected in MGCs. No versican cleavage products were detected in OCCs cultured without follicular fluid. In summary, porcine OCCs are autonomous in producing and cleaving V1; the cleaved fragment of ~70 kDa VG1 is specific for formation of the expanded cumulus HA-rich ECM.

show abstract

Section: Discussionmentioning

confidence: 61%

Versican G1 Fragment Establishes a Strongly Stabilized Interaction with Hyaluronan-Rich Expanding Matrix during Oocyte Maturation

Nagyová

Salustri

Němcová

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…[4][5][6] In the cumulus cells that surround oocytes, 7 hyaluronan forms a specialized jelly-like structure that is crosslinked by accessory proteins and is critical for fertilization. 8,9 In soft connective tissues, hyaluronan is not grafted to cells but organized in entangled or crosslinked hydrogels with the help of accessory proteins. In cartilage and in the vitreous humor, hyaluronan is for instance found together with collagen fibrils and aggrecans.…”

Section: Introductionmentioning

confidence: 99%

Particle diffusion in extracellular hydrogels

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Before ovulation, CCs regulate the level of cyclic adenosine monophosphate in oocytes to promote oocyte meiosis, changes in cumulus morphology and cumulus expansion to affect oocyte maturation. After ovulation, CCs affect spermoocyte binding and embryo development [31][32][33]. erefore, cumulus expansion is a prerequisite for oocyte Evidence-Based Complementary and Alternative Medicine maturation and fertilization, and can also be used as a morphological indicator to predict oocyte quality [34,35].…”

Section: Discussionmentioning

confidence: 99%

Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK3/1 to Promote the Expression of Cumulus Expansion‐Related Factors in Mice

Liang

Xue

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. Bushen Tiaojing Decoctions (BSTJ-II-D and BSTJ-III-D) are used to assist pregnancy in clinical practice. In this study, we explored the ability of sequential administration of BSTJ-II-D and BSTJ-III-D to promote cumulus cell (CC) expansion and its underlying mechanisms in controlled ovarian hyperstimulation (COH) mice. Methods. Kunming mice were randomly divided into three groups. The normal group was injected intraperitoneally with saline, and distilled water was administered orally by gavage. As the COH model, mice were injected with GnRHa, eCG, and hCG. Subsequently, the BSTJD group received BSTJ-II-D and BSTJ-III-D orally by gavage, while the control group received distilled water. We evaluated CC expansion and oocyte first polar body (PB1) extrusion under a stereomicroscope. Serum levels of follicle-stimulating hormone (FSH) were detected by radioimmunoassay. The expression of the CC expansion-related factors PTX3 and PTGS2 was detected by immunofluorescence, western blot, and quantitative real-time-polymerase chain reaction analyses (qRT-PCR). Expression of p-MAPK14, p-MAPK3/1, MAPK14, and MAPK3/1 was detected by western blot analysis. Results. Sequential administration of BSTJ-II-D and BSTJ-III-D promoted cumulus expansion and oocyte PB1 extrusion and upregulated PTX3 and PTGS2 expression at the mRNA and protein levels. Furthermore, the levels of p-MAPK14/MAPK14, p-MAPK3/1/MAPK3/1 proteins, and serum FSH in the BSTJD group were higher than those in the normal and control groups. Conclusions. Sequential administration of BSTJ-II-D and BSTJ-III-D promotes cumulus expansion and oocyte maturation in COH mice by increasing FSH expression and activating the MAPK14 and MAPK3/1 signalling pathways, thereby increasing expression of PTX3 and PTGS2.

show abstract

Molecular organization and mechanical properties of the hyaluronan matrix surrounding the mammalian oocyte

Cited by 31 publications

References 112 publications

Versican G1 Fragment Establishes a Strongly Stabilized Interaction with Hyaluronan-Rich Expanding Matrix during Oocyte Maturation

Versican G1 Fragment Establishes a Strongly Stabilized Interaction with Hyaluronan-Rich Expanding Matrix during Oocyte Maturation

Particle diffusion in extracellular hydrogels

Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK3/1 to Promote the Expression of Cumulus Expansion‐Related Factors in Mice

Contact Info

Product

Resources

About