Molecular network including eIF1AX, RPS7, and 14-3-3γ regulates protein translation and cell proliferation in bovine mammary epithelial cells

Yu, Chang Yeon; Qu, Bo; Khudhair, Nagam; Gu, Xinyu; Zang, Yanli; Wang, Chunmei; Zhang, Na; Qing-zhang, LI; Gao, Xuejun

doi:10.1016/j.abb.2014.09.014

Cited by 26 publications

(27 citation statements)

References 61 publications

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“…Our previous findings showed that 14-3-3γ could positively affect mTOR pathway (Yu et al 2014), which is essential for milk protein synthesis in dairy cow (Bionaz and Loor 2011;Zhang et al 2014). Further study on the immunoprecipitation of 14-3-3γ with 44 proteins in bovine mammary epithelial cells (BMECs) revealed that 14-3-3γ could interact with and positively affect the expression of eIF1AX (eukaryotic translation initiation factor 1A, X-linked) and RPS7 (ribosomal protein S7) to enhance milk protein synthesis (Yu et al 2014). These results suggest that 14-3-3γ is an important signaling molecule for milk protein synthesis through interacting with and regulating eIF1AX and RPS7 in the translational level.…”

Section: Introductionmentioning

confidence: 95%

“…The cell purity and milk protein synthesis ability of the primary BMECs were evaluated by the immunofluorescence detection of the expression of cytokeratin 18 and β-casein using methods as reported (Zavizion et al 1996;Yu et al 2014), and the purified BMECs in 0-10 passages were used for cell culture experiments.…”

Section: Tissue Samples and Cell Culturementioning

confidence: 99%

“…It has been published that eIF5 can promote the accuracy of start codon recognition (Saini et al 2014). eIF5 was one of the 44 proteins immunoprecipitated with 14-3-3γ (Yu et al 2014). We hypothesized that 14-3-3γ may interact with and regulate eIF5 to regulate milk protein synthesis in BMECs.…”

Section: Introductionmentioning

confidence: 96%

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14-3-3γaffects eIF5 to regulateβ-casein synthesis in bovine mammary epithelial cells

Luo

et al. 2016

Can. J. Anim. Sci.

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The 14-3-3γ protein participates in many biological processes; however, its regulatory mechanism in milk protein synthesis is not well studied. We hypothesized that 14-3-3γ might affect eIF5 (an initiation factor) to regulate β-casein synthesis in dairy cows. In this study, a possible interaction between 14-3-3γ and eIF5 was investigated using bovine mammary epithelial cells (BMECs). The expression levels of 14-3-3γ and eIF5 in the mammary gland tissues from cows producing higher quality milk were higher than those from cows producing low-quality milk. Moreover, the expression of 14-3-3γ, eIF5, and β-casein were increased at both mRNA and protein levels in BMECs cultured in vitro with methionine (Met) supplementation. Coimmunoprecipitation, colocalization, and FRET analysis further showed the evidences that 14-3-3γ physically bound to eIF5 in BMECs. Gene function studies revealed that 14-3-3γ positively regulated eIF5 through alteration of eIF2α/p-eIF2α ratio. Collectively, our data suggest that 14-3-3γ regulates β-casein translation in BMECs through interaction with eIF5.

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Section: Introductionmentioning

confidence: 95%

Section: Tissue Samples and Cell Culturementioning

confidence: 99%

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14-3-3γaffects eIF5 to regulateβ-casein synthesis in bovine mammary epithelial cells

Luo

et al. 2016

Can. J. Anim. Sci.

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“…Western blot analysis was performed as previously described (C. Yu et al, 2014). Briefly, 20 μg of protein equivalents of whole-cell extracts were extracted by WB Super RIPA Lysis Buffer (C2501;…”

Section: Western Blot Analysismentioning

confidence: 99%

NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway

Yuan

Zhang

et al. 2019

Journal Cellular Physiology

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Nuclear ubiquitous casein and cyclin‐dependent kinase substrate 1 (NUCKS1) is a highly phosphorylated nuclear protein ubiquitously expressed in vertebrates. NUCKS1 has been reported to be a key chromatin modifier and transcriptional regulator of a number of signaling pathways, but the physiological role and detailed mechanism are still limited. In this study, we assessed the role of NUCKS1 on milk synthesis in and proliferation of mammary epithelial cells from a dairy cow. NUCKS1 was located in the nucleus of mammary epithelial cells, and the expression of NUCKS1 was stimulated by amino acids (Met and Leu) and hormones (estrogen and prolactin). Gene function study approaches detected that NUCKS1 positively regulated milk protein, milk fat, and lactose synthesis, and also increased the cell number, cell viability, and cell cycle progression. NUCKS1 mediated the stimulation of amino acids and hormones on the messenger RNA expression of the mechanistic target of rapamycin (mTOR), SREBP‐1c, and Cyclin D1. The expression of NUCKS1 is dramatically higher in mouse mammary tissue of lactating period, compared with that in puberty and dry period. Taken together, these results reveal that NUCKS1 is a new mediator of milk synthesis in and proliferation of mammary epithelial cells via regulating the mTOR signaling pathway.

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“…Recent studies in bovines have highlighted a role of mTOR in the regulation of milk synthesis (Zhang et al, 2014), including milk protein synthesis (Wang et al, 2014b; Yu et al, 2014) and triacylglycerol or FAs synthesis (Li et al, 2014). Compared with untreated control cells, rapamycin reduced triglyceride secretion of BMECs (Zhang et al, 2014).…”

Section: Disscussionmentioning

confidence: 99%

Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells

Guo

Wang

Feng

et al. 2016

Asian Australas. J. Anim. Sci

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Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, including de novo lipid biosynthesis. Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene and the product of which was thought to be associated with elongation of carbon (C) chain in fatty acids. In the present study, we examined the effects of rapamycin, a specific inhibitor of mTORC1, on ELOVL1 expression and docosahexaenoic acid (DHA, C22:6 n-3) synthesis in bovine mammary epithelial cells (BMECs). We found that rapamycin decreased the relative abundance of ELOVL1 mRNA, ELOVL1 expression and the level of DHA in a time-dependent manner. These data indicate that ELOVL1 expression and DHA synthesis are regulated by mTORC1 in BMECs.

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Molecular network including eIF1AX, RPS7, and 14-3-3γ regulates protein translation and cell proliferation in bovine mammary epithelial cells

Cited by 26 publications

References 61 publications

14-3-3γaffects eIF5 to regulateβ-casein synthesis in bovine mammary epithelial cells

14-3-3γaffects eIF5 to regulateβ-casein synthesis in bovine mammary epithelial cells

NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway

Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells

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