Molecular Monitoring of the Developmental Bacterial Community in the Gastrointestinal Tract of Japanese Infants

Songjinda, Prapa; Nakayama, Jiro; Kuroki, Yumiko; Tanaka, Shigemitsu; Fukuda, Sanae; Kiyohara, Chikako; Yamamoto, Tetsuro; Izuchi, Kunio; Shirakawa, Taro; Sonomoto, Kenji

doi:10.1271/bbb.69.638

Cited by 25 publications

(26 citation statements)

References 7 publications

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“…Especially, it is known that E. faecium are more resistant to β‐lactams than E. faecalis due to overproduction of penicillin‐binding protein or production of β‐lactamase (Leclercq, 1997). Also in this study, DGGE displayed unusual colonization of E. faecium in the AT subjects while enterococci colonizing in our AF subjects mostly belong E. faecalis (Songjinda et al , 2005).…”

Section: Discussionsupporting

confidence: 53%

Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota

Tanaka

Kobayashi

Songjinda

et al. 2009

FEMS Immunology &Amp; Medical Microbiology

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341

233

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The influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota was monitored in 26 infants including five antibiotic-treated (AT) subjects orally administered a broad-spectrum antibiotic for the first 4 days of life and three caesarean-delivered (CD) subjects whose mothers were intravenously injected by the similar type of antibiotics in the same period. The faecal bacterial composition was analysed daily for the first 5 days and monthly for the first 2 months. Terminal restriction fragment length polymorphisms in the AT subjects showed less diversity with the attenuation of the colonization of some bacterial groups, especially in Bifidobacterium and unusual colonization of Enterococcus in the first week than the control antibiotic-free infants (AF, n = 18). Quantitative real-time PCR showed overgrowth of enterococci (day 3, P = 0.01; day 5, P = 0.003; month 1, P = 0.01) and arrested growth of Bifidobacterium (day 3, P = 0.03) in the AT group. Furthermore, after 1 month, the Enterobacteriaceae population was markedly higher in the AT group than in the AF group (month 1, P = 0.02; month 2, P = 0.02). CD infants sustained similar, although relatively weaker, alteration in the developing microbiota. These results indicate that antibiotic exposure at the beginning of life greatly influences the development of neonatal intestinal microbiota.

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Section: Discussionsupporting

confidence: 53%

Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota

Tanaka

Kobayashi

Songjinda

et al. 2009

FEMS Immunology &Amp; Medical Microbiology

Self Cite

341

233

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“…In a culture-based study from rural Guatemala, half of the neonates had E. coli during the first 24 h and all (n ϭ 30) harbored this bacterium at their second day of life (5). Other investigations have found Enterobacteriaceae and E. coli in various amounts (2,3). Recently, E. coli in pregnant women was shown to positively correlate with neonate birth weight (38), which in turn, increases the risk of metabolic disturbances later in life (17,18).…”

Section: Discussionmentioning

confidence: 99%

The Pioneer Gut Microbiota in Human Neonates Vaginally Born at Term—A Pilot Study

et al. 2011

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“…However, other studies based on DNA, apart from ours, have also reported a high prevalence of Bifidobacterium (17, 51, 52). Lack of breastfeeding, antibiotics, preterm birth, and cesarean delivery are the factors, which have been associated with a lack of or reduction in Bifidobacterium species in infants (22, 24, 45, 53). The relative lack of Bifidobacterium in some of the recent studies may therefore instead reflect differential exposure to gut microbiota‐modifying factors.…”

Section: Discussionmentioning

confidence: 99%

Development of gut microbiota in infants not exposed to medical interventions

Eggesbø

Moen

Peddada

et al. 2010

Apmis

143

112

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Summary Knowledge of the composition of a normal healthy gut microbiota during infancy is important for understanding the role of gut microbiota in disease. A limitation of previous studies is that they are based on infants who have been subject to factors which can have a profound disruptive effect on the natural colonization process. We describe the colonization process, during the first four months after birth, in 85 infants who have experienced no major medical or dietary interventions. They were all vaginally delivered, healthy, term infants, who were not exposed to antibiotics, exclusively breastfed during their first month of life and at least partially breastfed up to four months. Selected microbial groups were identified by targeting small subunit microbial ribosomal RNA genes. In contrast to more recent studies, but in agreement with older studies, almost all our infants harbored γ-proteobacteria and Bifidobacterium..Yet undefined non-cultivable species belonging to Bacteroides, as well as microbes identified as Lachnospiraceae 2, were common. Strong associations were observed between some specific constituents of microbiota at day 4 and the concentration of specific microbial groups at day 120, indicating that early gut microbiota may influence later microbiota. Novel information of the undisturbed composition of early gut microbiota in babies is presented.

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Molecular Monitoring of the Developmental Bacterial Community in the Gastrointestinal Tract of Japanese Infants

Cited by 25 publications

References 7 publications

Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota

Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota

The Pioneer Gut Microbiota in Human Neonates Vaginally Born at Term—A Pilot Study

Development of gut microbiota in infants not exposed to medical interventions

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