Molecular monitoring of bulking sludge in industrial wastewater treatment plants

Waarde, J. van der; Krooneman, Janneke; Geurkink, Bert; Werf, A. van der; Eikelboom, Dick H.; Beimfohr, Claudia; Snaidr, Jiri; Levantesi, Caterina; Tandoi, Valter

doi:10.2166/wst.2002.0533

Cited by 27 publications

(24 citation statements)

References 13 publications

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“…In 13 % of the samples, the FI was above 2.5, indicating their potential involvement in bulking in these WWTPs. These results confirmed a previous FISH survey on a large number of industrial WWTP (van der Waarde et al, 2002).…”

Section: Identity and Abundancesupporting

confidence: 91%

“…A species-specific probe (HHY) targeting H. hydrossis was designed based on the type strain DSM1100 (Wagner et al, 1994). Only one comprehensive FISH survey has been carried out using this probe (van der Waarde et al, 2002) and H. hydrossis was very frequently observed (present in 58 % of the samples), but its abundance in these samples was not estimated. Furthermore, four additional filamentous organisms with a H. hydrossis-like morphology (but HHY-negative) were commonly observed , but the phylogenetic affiliation of these filaments was never resolved.…”

Section: Introductionmentioning

confidence: 99%

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Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Kragelund

Levantesi

Borger³

et al. 2008

Microbiology

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Filamentous members of the Bacteroidetes are commonly observed in activated sludge samples originating from both municipal and industrial wastewater treatment plants (WWTP), where they occasionally can cause bulking. Several oligonucleotide 16S rRNA-targeted probes were designed to target filaments with a needle-like appearance similar to Haliscomenobacter hydrossis. The design of these probes was based on an isolate and a sequence obtained from a micromanipulated filament. The abundance of filamentous Bacteroidetes was determined in 126 industrial samples applying already published and the newly developed probes. Small populations were found in 62 % of the WWTP investigated. However, only relatively few WWTP (13 %) contained large populations of filamentous Bacteroidetes potentially responsible for bulking incidences. The identity of the most abundant filamentous Bacteroidetes with H. hydrossis morphology could be detected by probes CFB719, SAP-309 and the newly designed probe HHY-654. A comprehensive study on the ecophysiology of probe-defined Bacteroidetes populations was conducted on Danish and Czech samples. The studies revealed that they were specialized bacteria involved in degradation of sugars, e.g. glucose and N-acetylglucosamine, and may participate in the conversion of lipopolysaccharides and peptidoglycan liberated by decaying cells. Many surface-associated exo-enzymes were excreted, e.g. chitinase, glucuronidase, esterase and phosphatase, supporting conversion of polysaccharides and possibly other released cell components. The role of filamentous bacteria with a H. hydrossis-like morphology in the activated sludge ecosystem is discussed.

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Section: Identity and Abundancesupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Kragelund

Levantesi

Borger³

et al. 2008

Microbiology

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“…This might be the case, for example, of Sphaerotilus which were present in the inoculum of RC and R1. These microorganisms are known to appear occasionally in plants with nutrient deficiency [11,31]. This theory would explain the presence of filamentous organism in the surface of the aerobic granules due to oxygen limiting conditions which has been found frequently in this kind of systems [5] when high organic loads are treated.…”

Section: Effects Of Operational Conditionsmentioning

confidence: 98%

“…Another organism like Thiothrix was identified in systems treating different municipal and industrial wastewaters with and without nutrient removal [31]. Wastewaters rich in reduced sulphur compounds, nutrients deficiency or in systems operated with low feeding to microorganisms (F/M) ratios are environments where Thiothrix is commonly found [7,37].…”

Section: Effects Of Operational Conditionsmentioning

confidence: 99%

Filamentous bacteria existence in aerobic granular reactors

Figueroa

Río

Campos

et al. 2014

Bioprocess Biosyst Eng

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Filamentous bacteria are associated to biomass settling problems in wastewater treatment plants. In systems based on aerobic granular biomass they have been proposed to contribute to the initial biomass aggregation process. However their development on mature aerobic granular systems has not been sufficiently studied. In the present research work filamentous bacteria were studied for the first time after long term operation (up to 300 days) of aerobic granular systems. Chloroflexi and Sphaerotilus natans have been observed in a reactor fed with synthetic wastewater. These filamentous bacteria could only come from the inoculated sludge. Thiothrix and Chloroflexi bacteria were observed in aerobic granular biomass treating wastewater from a fish canning industry. Meganema perideroedes was detected in a reactor treating wastewater from a plant processing marine products. As a conclusion the source of filamentous bacteria in these mature aerobic granular systems fed with industrial effluents was the incoming wastewater.

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“…Filamentous Alphaproteobacteria have been shown to be very important in industrial WWTPs where they are often associated with bulking incidents or deteriorating settling properties of the sludge (Levantesi et al, 2004;van der Waarde et al, 2002). The filamentous Alphaproteobacteria have been observed and described in several studies in the past 4 years (Dionisi et al, 2002;Levantesi et al, 2004;Thomsen et al, 2006;van der Waarde et al, 2002) and, based on 16S rRNA gene sequences from six uncultured bacteria and one isolated species, they have now been described as constituting seven distantly related phylogenetic clusters: 'Candidatus Combothrix italica', 'Candidatus Catenimonas italica', 'Candidatus Sphaeronema italicum', 'Candidatus Alysiosphaera europaea', 'Candidatus Monilibacter batavus', 'Candidatus Alysiomicrobium bavaricum' (Levantesi et al, 2004) and Meganema perideroedes (Thomsen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Ecophysiology of different filamentous Alphaproteobacteria in industrial wastewater treatment plants

et al. 2006

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The ecophysiology of five filamentous species affiliated to the Alphaproteobacteria was investigated in industrial activated sludge systems. The five species, ‘Candidatus Alysiosphaera europaea’, ‘Candidatus Monilibacter batavus’, ‘Candidatus Alysiomicrobium bavaricum’, ‘Candidatus Sphaeronema italicum’ and Meganema perideroedes, are very abundant in industrial wastewater treatment plants and are often involved in bulking incidents. The morphology of these filamentous bacterial species resembled Eikelboom's Nostocoida limicola, or Type 021N, and could only be correctly identified by using fluorescence in situ hybridization (FISH), applying species-specific gene probes. Two physiological groupings of the five species were found using microautoradiography combined with FISH. Group 1 (‘Ca. Monilibacter batavus' and ‘Ca. Sphaeronema italicum’) utilized many short-chained fatty acids (acetate, pyruvate and propionate), whereas Group 2 (‘Ca. Alysiosphaera europaea’, ‘Ca. Alysiomicrobium bavaricum’ and Meganema perideroedes) could also exploit several sugars, amino acids and ethanol. All species had polyhydroxyalkanoate granules present and several of the species had a very large storage capacity. No activity was found under strict anaerobic conditions, while uptake of substrate was observed in the presence of nitrate or nitrite as potential electron acceptor. However, for all species a reduced number of substrates could be consumed under these conditions compared to aerobic conditions. Only a little exo-enzymic activity was found and nearly all species had a hydrophobic cell surface. Based on knowledge of the ecophysiological potential, control strategies are suggested.

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Molecular monitoring of bulking sludge in industrial wastewater treatment plants

Cited by 27 publications

References 13 publications

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Filamentous bacteria existence in aerobic granular reactors

Ecophysiology of different filamentous Alphaproteobacteria in industrial wastewater treatment plants

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