Molecular module of <scp>CmMYB15‐like‐Cm4CL2</scp> regulating lignin biosynthesis of chrysanthemum (<i>Chrysanthemum morifolium</i>) in response to aphid (<i>Macrosiphoniella sanborni</i>) feeding

Li, Fēi; Zhang, Yi; Tian, Chang; Wang, Xinhui; Zhou, Lijie; Jiang, Jiafu; Wang, LiKai; Chen, Fadi; Chen, Sumei

doi:10.1111/nph.18643

Cited by 18 publications

(16 citation statements)

References 63 publications

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“…Afterward, the vectors of CaLCuV, PCVB, and CaLCuV‐ CmAFL1 were transformed into A. tumefaciens strain GV3101, respectively. The preparation of the infection suspension and the procedures for instantaneous conversion of chrysanthemums were performed as described before (Huang et al., 2022; Li et al., 2023).…”

Section: Methodsmentioning

confidence: 99%

“…Afterward, the vectors of CaLCuV, PCVB, and CaLCuV-CmAFL1 were transformed into A. tumefaciens strain GV3101, respectively. The preparation of the infection Ó 2023 Society for Experimental Biology and John Wiley & Sons Ltd., The Plant Journal, (2023), 116, 1652-1666 CmTEM1-CmAFL1 module inhibits flowering 1663 suspension and the procedures for instantaneous conversion of chrysanthemums were performed as described before (Huang et al, 2022;Li et al, 2023).…”

Section: Virus-induced Gene Silencing Experiments In Chrysanthemummentioning

confidence: 99%

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The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

Cheng,

Wang,

Zhang

et al. 2023

The Plant Journal

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SUMMARYTEMPRANILLO1 (TEM1) is a transcription factor belonging to related to ABI3 and VP1 family, which is also known as ethylene response DNA‐binding factor 1 and functions as a repressor of flowering in Arabidopsis. Here, a putative homolog of AtTEM1 was isolated and characterized from chrysanthemum, designated as CmTEM1. Exogenous application of ethephon leads to an upregulation in the expression of CmTEM1. Knockdown of CmTEM1 promotes floral initiation, while overexpression of CmTEM1 retards floral transition. Further phenotypic observations suggested that CmTEM1 involves in the ethylene‐mediated inhibition of flowering. Transcriptomic analysis established that expression of the flowering integrator CmAFL1, a member of the APETALA1/FRUITFULL subfamily, was downregulated significantly in CmTEM1‐overexpressing transgenic plants compared with wild‐type plants but was verified to be upregulated in amiR‐CmTEM1 lines by quantitative RT‐PCR. In addition, CmTEM1 is capable of binding to the promoter of the CmAFL1 gene to inhibit its transcription. Moreover, the genetic evidence supported the notion that CmTEM1 partially inhibits floral transition by targeting CmAFL1. In conclusion, these findings demonstrate that CmTEM1 acts as a regulator of ethylene‐mediated delayed flowering in chrysanthemum, partly through its interaction with CmAFL1.

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Section: Methodsmentioning

confidence: 99%

Section: Virus-induced Gene Silencing Experiments In Chrysanthemummentioning

confidence: 99%

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

Cheng,

Wang,

Zhang

et al. 2023

The Plant Journal

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“…Different classes of avonoids and lignin are synthesized via the phenylpropanoid pathway [49]. Lignin is a structural component of the cell wall and the expression of its biosynthetic pathway genes enhance plant defense [50][51][52]. The expression of genes encoding key enzymes of the phenylpropanoid pathway was signi cantly upregulated following A. alternata infection in chrysanthemum (Fig.…”

Section: Ja Enhanced the Cell Wall Integrity Maintenance Systemmentioning

confidence: 99%

Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum

Zhang

Miao

Liu

et al. 2023

Preprint

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Background Black spot disease caused by the necrotrophic fungus Alternaria spp. is one of the most devastating diseases affecting Chrysanthemum morifolium. There is currently no effective way to prevent chrysanthemum black spot. Results We revealed that pre-treatment of chrysanthemum leaves with the plant hormone jasmonate (JA) significantly reduces their susceptibility to Alternaria alternata. To understand how JA treatment induces resistance, we monitored the dynamics of metabolites and the transcriptome in leaves after JA treatment following A. alternata infection. JA signaling affected the resistance of plants to pathogens through cell wall modification, Ca2+ regulation, reactive oxygen species (ROS) regulation, mitogen-activated protein kinase cascade and hormonal signaling processes, and the accumulation of anti-fungal and anti-oxidant metabolites. Furthermore, the expression of genes associated with these functions was verified by reverse transcription quantitative PCR and transgenic assays. Conclusion Our findings indicate that JA pre-treatment could be a potential orchestrator of a broad-spectrum defense response that may help establish an ecologically friendly pest control strategy and offer a promising way of priming plants to induce defense responses against A. alternata.

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“…In addition to providing structural support, lignin is involved in plant defense against biotic and abiotic stresses (Kumar, Campbell, et al, 2016;Moura et al, 2010;Wang, Guo, et al, 2022). Studies have shown that the enhanced lignin biosynthesis in plants contributes to their resistance against citrus canker and aphid infestation (An et al, 2019;Li, Zhang, et al, 2023;Zhou, Yue, et al, 2021). Improved lignin content also enhances the drought and salt tolerance of plants (Chen, Guo, et al, 2020;Li, Yang, et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

CsPAT1, a GRAS transcription factor, promotes lignin accumulation by antagonistic interacting with CsWRKY13 in tea plants

Li,

Zhou,

et al. 2024

The Plant Journal

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SUMMARYLignin is an important component of plant cell walls and plays crucial roles in the essential agronomic traits of tea quality and tenderness. However, the molecular mechanisms underlying the regulation of lignin biosynthesis in tea plants remain unclear. CsWRKY13 acts as a negative regulator of lignin biosynthesis in tea plants. In this study, we identified a GRAS transcription factor, phytochrome A signal transduction 1 (CsPAT1), that interacts with CsWRKY13. Silencing CsPAT1 expression in tea plants and heterologous overexpression in Arabidopsis demonstrated that CsPAT1 positively regulates lignin accumulation. Further investigation revealed that CsWRKY13 directly binds to the promoters of CsPAL and CsC4H and suppresses transcription of CsPAL and CsC4H. CsPAT1 indirectly affects the promoter activities of CsPAL and CsC4H by interacting with CsWRKY13, thereby facilitating lignin biosynthesis in tea plants. Compared with the expression of CsWRKY13 alone, the co‐expression of CsPAT1 and CsWRKY13 in Oryza sativa significantly increased lignin biosynthesis. Conversely, compared with the expression of CsPAT1 alone, the co‐expression of CsPAT1 and CsWRKY13 in O. sativa significantly reduced lignin accumulation. These results demonstrated the antagonistic regulation of the lignin biosynthesis pathway by CsPAT1 and CsWRKY13. These findings improve our understanding of lignin biosynthesis mechanisms in tea plants and provide insights into the role of the GRAS transcription factor family in lignin accumulation.

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Molecular module of CmMYB15‐like‐Cm4CL2 regulating lignin biosynthesis of chrysanthemum (Chrysanthemum morifolium) in response to aphid (Macrosiphoniella sanborni) feeding

Cited by 18 publications

References 63 publications

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum

CsPAT1, a GRAS transcription factor, promotes lignin accumulation by antagonistic interacting with CsWRKY13 in tea plants

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