Molecular modification of Penaeus monodon sperm in female thelycum and its consequent responses

Abstract

Using Penaeus monodon as the model, we demonstrated the molecular changes and the mechanism of thelycal-dependent sperm modification resulting in an enhanced acrosome reaction (AR) response. Attention was paid to the modification of the sperm plasma membrane which was mediated through an adsorption or removal of sperm peripheral and integral membrane proteins as indicated by the different profiles of these proteins in spermatophore (S) and thelycal (T) sperm. In vitro adsorption of Alexa-488 conjugated T proteins onto the entire S-sperm surface confirmed protein transfer in a time-dependent manner. Specific anchoring of 83 and 140 kDa proteins to sperm peripheral proteins as well as 53/55 and 60 kDa proteins to sperm lipids was demonstrated. Apart from membrane modification, a substantial increase in protein tyrosine phosphorylation was shown to be closely associated with T-dependent sperm modification event. The physiological significance of this sperm modification in enhancing sperm AR response, which required at least 3 days of T residence in order for the sperm to gain a complete AR response, was also elucidated.

Label-free protein quantification in freshly ejaculated versus post-mating spermatophores of the noble crayfish Astacus astacus

“…In Astacidae and Parastacidae, spermatophores are deposited on the ventral surface of the female, where they remain for several days before initiation of ovulation and subsequent fertilization [1,2,3]. This is accompanied by spermatophore morphological changes [4,5,6], suggesting that final maturation of decapod spermatozoa takes place in the seminal receptacle of the female [7,8,9,10].…”

Post-mating morphological changes in the spermatozoon and spermatophore wall of the crayfish Astacus leptodactylus: Insight into a non-motile spermatozoon

“…It can be hypothesized that the post-mating contact of water with the crayfish spermatophore wall induces reticulation of the grainy matrix. Therefore, contents of the granules can be released and reach the sperm mass via porous texture of the reticulated grainy matrix, and the substances released may be acting synergistically with factors from the female reproductive tracts (Vanichviriyakit et al, 2004) or independently for final maturation and capacitation of spermatozoa.…”

“…The spermatophores remain for several days with the female before initiation of ovulation and subsequent fertilization (Vogt, 2002). This is accompanied by morphological changes (Dudenhausen and Talbot, 1983;López-Greco and Lo Nostro, 2008), suggesting that final maturation and capacitation of a decapod spermatozoon takes place in the seminal receptacle of the female (Alfaro et al, 2003(Alfaro et al, , 2007Aungsuchawan et al, 2011;Vanichviriyakit et al, 2004).…”

Different aspects of reproduction strategies in crayfish: A review

“…The freshly ejaculated spermatophore is soft in decapods, but after mating it becomes hardened to protect the sperm mass (Malek and Bawab, 1971;Uma and Subramoniam, 1979). Post-mating spermatophore storage in crayfish and other decapods is accompanied by morphological and molecular changes that are necessary for the spermatozoon to develop fertilizing ability (Alfaro et al, 2003;Vanichviriyakit et al, 2004;Alfaro et al, 2007;López Greco and Lo Nostro, 2008;Aungsuchawan et al, 2011;Braga et al, 2014;Niksirat et al, 2014bNiksirat et al, , 2015b.…”