Molecular miR-19a in Acute Myocardial Infarction: Novel Potential Indicators of Prognosis and Early Diagnosis

Abstract

Objective: Due to the increasing annual incidence rate of disability and mortality in patients with acute myocardial infarction (AMI), the need for an appropriate diagnostic tool has become a crucial urgent issue. An increase in biomarkers and protein levels in response to AMI can be used as a predictive biomarker with different sensitivities and specificities. This study aimed at investigating the role of miR-19a as a biomarker with acceptable sensitivity and specificity for early diagnosis of AMI. Methods: We studied 175 patients with AMI admitted within 12 h of symptom onset and 90 healthy subjects as control group. Patients were divided into two groups, including group I (normal vessels and no significant artery stenosis) and primary percutaneous coronary intervention (PCI) group II (patients with more than 50% stenosis in vessels and severe atherosclerosis) diagnosed by angiography. The expression level of miR-19a was evaluated by the real-time polymerase chain reaction and other serum chemistries were also analyzed. Results: The results demonstrated that circulating miR-19a levels were significantly increased in patient groups compared to the control group (2.88 ± 1.06 vs. 5.93 ± 1.28, P<0.0001). We also found that miR-19a levels were higher in group II (134.62-fold) than group I (15.42-fold). The upper levels of miR-19a were significantly correlated with the increased serum levels of CK-MB (ρ=0.29, P<0.0001), CTn I (ρ=0.4, P<0.0001) and creatinine (ρ=0.27, P<0.0001). In addition, Receiver Operating Characteristic (ROC) analysis revealed that circulating miR-19a had considerable diagnostic accuracy for the patients with normal vessel with an AUC of 0.930 (95% CI: 0.697-0.765) and for PCI patients with an AUC of 0.966 (95% CI: 0.748-0.784). Conclusion: Circulating miR-19a possibly has prognostic value to be used as a promising molecular target for early diagnosis and prognosis of AMI.

Dexmedetomidine‐up‐regulated microRNA‐381 exerts anti‐inflammatory effects in rats with cerebral ischaemic injury via the transcriptional factor IRF4

“…The total RNA was extracted using the RNeasy Mini Kit (Qiagen). The complementary DNA (cDNA) of miRNA was obtained by the reverse transcription of miRNA using the miRNA First Strand cDNA Synthesis (Tailing Reaction) kit (B532453‐0020; Sangon Biotech) and that of mRNA by the reverse transcription of mRNA using the reverse transcription kit (RR047A; Takara Bio), respectively 9 . RT‐qPCR was performed on a Bio‐Rad instrument (10 021 337; Bio‐Rad).…”

“…Besides, other studies have illustrated the protective effect of Dex on the ischaemic or reperfusion injury of various organs including the heart and kidney 8 . On the other hand, recent studies have indicated the prognostic value of circulating miR‐19a and suggested it as a promising molecular target for early diagnosis and prognosis of acute myocardial infarction 9 . Though microRNAs (miRNAs), that is miR‐129‐5p 10 and miR‐29b, 11 have indicated being involved in the neuroprotective effect of Dex.…”

Stem Cell Therapy Potency in Personalizing Severe COVID-19 Treatment

“…Some biomolecules can be accumulated into exosomes that are regularly released from the blood. The role of exosomes as a vesicle to secrete and store biological molecules is crucial [ 66 ]. Hence, MSC-secreted exosomes have been increasingly recognized as a multicomplex paracrine factor that carries different biomolecules, for instance, a series of uncoded miRNAs like miR-34a, miR-200b/c, miR-146a, and Let-7 which are classified via their interaction with proinflammatory mediators.…”

Electrospun Polycaprolactone Nanofibers: Current Research and Applications in Biomedical Application

“…Disability in patients is associated with the severity of the cardiac defect and reflected the degree of one or more cardiac artery stenosis or cardiac region ischemia. Each year, many reconstructive surgical procedures and invasive bypass surgery are performed to repair heart-tissue defects, traumatic cardiovascular injury, and congenital defect 55 . The standard care based on electrospun PCL for heart tissue reconstruction is responsible for restoration in injured myocardium cells, leading to increased blood supply in injured myocardium or local angiogenesis.…”