Molecular Methods for Haemophilus influenzae

Ma, Herbert; Crook, D; Moxon, E. Richard

doi:10.1385/0-89603-498-4:243

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…Haemophilus influenzae is non-motile Gram negative of the family Pasteurellaceae that is pleomorphic in shape, with morphologies ranging from coccobacillus to filamentous bacillus (Herbert, Crook and Moxon 1998). Haemophilus influenzae is a fastidious facultative anaerobe that requires both Factor X (hemin) and Factor V (nicotinamide adenine dinucleotide-NAD) for growth.…”

Section: Characteristics and Identification Of Haemophilus Influenzaementioning

confidence: 99%

Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease

Ahearn

Gallo

Murphy

2017

Pathogens and Disease

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Non-typeable Haemophilus influenzae (NTHi) is the most common bacterial cause of infection of the lower airways in adults with chronic obstructive pulmonary disease (COPD). Infection of the COPD airways causes acute exacerbations, resulting in substantial morbidity and mortality. NTHi has evolved multiple mechanisms to establish infection in the hostile environment of the COPD airways, allowing the pathogen to persist in the airways for months to years. Persistent infection of the COPD airways contributes to chronic airway inflammation that increases symptoms and accelerates the progressive loss of pulmonary function, which is a hallmark of the disease. Persistence mechanisms of NTHi include the expression of multiple redundant adhesins that mediate binding to host cellular and extracellular matrix components. NTHi evades host immune recognition and clearance by invading host epithelial cells, forming biofilms, altering gene expression and displaying surface antigenic variation. NTHi also binds host serum factors that confer serum resistance. Here we discuss the burden of COPD and the role of NTHi infections in the course of the disease. We provide an overview of NTHi mechanisms of persistence that allow the pathogen to establish a niche in the hostile COPD airways.

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Section: Characteristics and Identification Of Haemophilus Influenzaementioning

confidence: 99%

Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease

Ahearn

Gallo

Murphy

2017

Pathogens and Disease

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“…Bacterial strains used in this study are listed in Table 2. NTHi and Escherichia coli strains were cultured using standard techniques described previously (4,61,62). NTHi was grown on chocolate agar plates (Remel) at 35°C and 5% CO 2 or in brain heart infusion broth supplemented with 10 g/ml of ␤-nicotinamide adenine dinucleotide (␤-NAD) (Sigma) and 10 g/ml of hemin (Sigma) at 37°C shaking at 225 rpm with or without antibiotics (sBHI).…”

Section: Methodsmentioning

confidence: 99%

Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion

et al. 2019

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Nontypeable Haemophilus influenzae (NTHi) is the primary cause of bacterially induced acute exacerbations of chronic obstructive pulmonary disease (COPD). NTHi adheres to and invades host respiratory epithelial cells as a means to persist in the lower airways of adults with COPD. Therefore, we mined the genomes of NTHi strains isolated from the airways of adults with COPD to identify novel proteins to investigate their role in adherence and invasion of human respiratory epithelial cells. An isogenic knockout mutant of the open reading frame NTHI1441 showed a 76.6% ± 5.5% reduction in invasion of human bronchial and alveolar epithelial cells at 1, 3, and 6 h postinfection. Decreased invasion of the NTHI1441 mutant was independent of either intracellular survival or adherence to cells. NTHI1441 is conserved among NTHi genomes. Results of whole-bacterial-cell enzyme-linked immunosorbent assay (ELISA) and flow cytometry experiments identified that NTHI1441 has epitopes expressed on the bacterial cell surface. Adults with COPD develop increased serum IgG against NTHI1441 after experiencing an exacerbation with NTHi. This study reveals NTHI1441 as a novel NTHi virulence factor expressed during infection of the COPD lower airways that contributes to invasion of host respiratory epithelial cells. The role in host cell invasion, conservation among strains, and expression of surface-exposed epitopes suggest that NTHI1441 is a potential target for preventative and therapeutic interventions for disease caused by NTHi.

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“…H. influenzae can be found, as a part of the normal microflora, in the upper respiratory tractespecially oropharynx and nasopharynx, of about 90% of adults. Therefore, H. influenzae may act as opportunistic pathogens, particularly in patients suffering from viral infections or compromised immune systems[ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Vaccine Candidates against Nontypeable Haemophilus influenzae: a Review

Behrouzi

Vaziri

Jamnani

et al. 2017

IBJ

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Nonencapsulated, nontypeable Hemophilus influenzae (NTHi) remains an important cause of acute otitis and respiratory diseases in children and adults. NTHi bacteria are one of the major causes of respiratory tract infections, including acute otitis media, cystic fibrosis, and community-acquired pneumonia among children, especially in developing countries. The bacteria can also cause chronic diseases such as chronic bronchitis and chronic obstructive pulmonary disease in the lower respiratory tract of adults. Such bacteria express several outer membrane proteins, some of which have been studied as candidates for vaccine development. Due to the lack of effective vaccines as well as the spread and prevalence of NTHi worldwide, there is an urgent need to design and develop effective vaccine candidates against these strains.

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Molecular Methods for Haemophilus influenzae

Cited by 4 publications

References 3 publications

Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease

Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease

Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion

Vaccine Candidates against Nontypeable Haemophilus influenzae: a Review

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